Medicinal plant extracts with efflux inhibitory activity against Gram-negative bacteria

Garvey, Mark I.; Rahman, M. Mukhlesur; Gibbons, Simon; Piddock, Laura J. V.

doi:10.1016/j.ijantimicag.2010.10.027

Cited by 115 publications

(93 citation statements)

References 40 publications

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“…The E. camaldulensis extract showed the best synergism with ceftriaxone (22mm) and (20mm), against S. aureus AM23 and P. aeruginosa AM41, respectively. It is speculated that inhibition of drug efflux, increasing permeability, inhibition of β-lactamase and alternative mechanisms of action could be responsible for the synergistic interactions between plant extracts and antibiotics (Garvey et al, 2011). Moreover, Kaur et al, (2016) reported that natural substances can produce synergistic effects, these combinations could enhance the efficacy of the other antimicrobial agents and acted as alternative to treating infections caused by multidrug-resistant microorganisms having no effective therapy (Adwan et al,2008).…”

Section: Resultsmentioning

confidence: 99%

Synergistic Effect of Combined Antibiotic and Methanol Extract of Eucalyptus camaldulensis leaf Against Staphylococcus aureus and Pseudomonas aeruginosa

Reda

El-Zawahry

Omar

2017

Int J Appl Sci Biotechnol

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A total of seventy-five medical specimens were collected from different patients admitted to Zagazig university hospital, Egypt. The selected bacterial isolates were distributed as 45 Gram negative bacterial isolates and 30 Gram positive bacterial isolates. The most effective antibiotic was gentamycin followed by amikacin and nitrofurantoin. The multi-drug resistant (MDR) bacterial isolates were selected and identified to four groups; Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and Staphylococcus aureus. The selected isolates were screened for their capability of biofilm, hemolysins, lecithinase and protease production. S. aureus AM23 and P. aeruginosa AM41 were selected as the highest biofilm and degrading enzymes producers. Furthermore, twenty-four methanolic and aqueous crude extracts derived from different medicinal plants in Egypt were screened for their antibacterial activity against both selected MDR strains. Methanolic extract of Eucalyptus camaldulensis leaf showed the greatest effect on the tested bacteria. Also, there was a synergistic effect of combined E. camaldulensis leaf extract and antibiotics (Gentamycin and Ceftriaxone) against tested organisms. Transmission electron microscopy showed changes in cell shapes, size, contents and cell envelop of antimicrobials treated bacterial cells compared to the control. Our findings proved that the leaf extracts of E. camaldulensis had great potential as antimicrobial agents in the treatment of infectious organisms.

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Section: Resultsmentioning

confidence: 99%

Synergistic Effect of Combined Antibiotic and Methanol Extract of Eucalyptus camaldulensis leaf Against Staphylococcus aureus and Pseudomonas aeruginosa

Reda

El-Zawahry

Omar

2017

Int J Appl Sci Biotechnol

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“…Also, some reports show that falcarindiol a fraction of the chloroform extracts of L. officinale L., and some other medicinal plants strongly inhibit the growth of different species of bacteria (10,19). In addition to its antibacterial property, this polyacetylene exhibits various biological activities such as antifungal and cytotoxic properties (19 Overall, it can be suggested that the association of L. officinale extracts and fluoroquinolones could be helpful to fight against infections based on the tested strains.…”

Section: Discussionmentioning

confidence: 99%

“…A report by Garvey et al (10) indicates that extracts of some herbal medicinal plants contain inhibitors of efflux in Gram-negative bacteria, the most active compound falcarindiol, showed synergistic activity with ciprofloxacin extracted from Levisticum officinale L.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and Antibiotic-Potentiation Activities of Levisticum officinale L. Extracts on Pathogenic Bacteria

et al. 2016

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Background: Multidrug resistant (MDR) Gram-negative bacteria have resistance to many antimicrobial compounds by multiple mechanisms including reduced outer membrane permeability, and active efflux mechanisms by efflux pumps. Objectives: The current study was planned to search the antibacterial activities of the ethanol and chloroform extracts of Levisticum officinale L., and their synergistic effects with ciprofloxacin against some Gram-negative pathogenic bacteria; also to analyze the extracts if they contain inhibitors of efflux pumps of the examined bacteria. Materials and Methods: After grinding, the resulting powder of Levisticum officinale L. was extracted with 85% ethanol and chloroform by maceration method. The studied microorganisms included the reference strains of Pseudomonas aeruginosa, Acinetobacter baumannii, Escherichia coli and Salmonella enteritidis. Broth micro-dilution methods were used to determine the minimum inhibitory concentrations (MICs) of the extracts alone or in association with ciprofloxacin and phenylalanine-arginine-β-naphtylamide (PAβN) as an efflux pump inhibitor (EPI). The presumptive efflux activity was detected by the ethidium bromide (EB) well diffusion method. Results: MIC determination indicated that the Levisticum officinale L. extracts inhibited the growth of all the studied bacteria within

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“…On the other hand, it cannot be unconsidered of other protection mechanisms are involved such as efflux pump, enzymatic modifications and modifications of target sites that can be resistant to microorganism metabolites (Garvey et al, 2011). The specific mechanisms involved in the antimicrobial action of terpenes remain poorly characterized (Helander et al, 1998;Silveira et al, 2006).…”

Section: Antimicrobial Activity (Model Agar Diffusion)mentioning

confidence: 99%

Myracrondruon urundeuva Allemo: Chemical composition, antioxidant activity, antimicrobial activity and inotropic effect

Santos¹,

Lima²,

Andrade³

et al. 2017

Afr. J. Biotechnol.

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Caatinga biome features species with high therapeutic potential such as Aroeira do Sertão (Myracrodruon urundeuva Allemão) which has long history in folk medicine. This study aimed to chemically characterize the secondary metabolites of hydroethanolic extract and hexane, chloroform, acetate, hydroethanolic fractions from Myracrodruon urundeuva (Allemão) possibly responsible antioxidant and antimicrobial activity, besides to evaluate the inotropic effect of crude extract in the left atrium of Cavia porcellus. Phytochemical composition was evaluated by spectrophotometry and highperformance liquid chromatography (HPLC). Antioxidant activity was assessed by 2,2-diphenyl-1-picryl hidrazyl (DPPH•) scavenging and reactive substances to thiobarbituric acid methods. Inotropic effect was evaluated using the isolated organ model. Data were expressed as mean ± SEM and differences determined by ANOVA followed by Tukey test. Phytochemical screening and the HPLC confirmed the presence of flavonoids, terpenes, tannins. Acetate and Hydroethanolic extract showed inhibition of DPPH values of 81.56 and 93.28% respectively. On the other hand, TBARS reduced in extract were 64.13% in a concentration of 150 µg/mL. Hydroethanolic extract and acetate fraction with concentrations of 50 and 100 mg/mL showed inhibition zones between 11.00 and 20.50 mm suggesting antimicrobial activity against Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumoniae bacteria and Candida albicans. The isolated atrium model demonstrated that the extract acts non-competitively antagonizing muscle calcium currents, decreasing the force of contraction by about 60%. Flavonoids, terpenes and tannins are responsible for M. urundeuva (Allemão) biological effects.

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Medicinal plant extracts with efflux inhibitory activity against Gram-negative bacteria

Cited by 115 publications

References 40 publications

Synergistic Effect of Combined Antibiotic and Methanol Extract of Eucalyptus camaldulensis leaf Against Staphylococcus aureus and Pseudomonas aeruginosa

Synergistic Effect of Combined Antibiotic and Methanol Extract of Eucalyptus camaldulensis leaf Against Staphylococcus aureus and Pseudomonas aeruginosa

Antibacterial and Antibiotic-Potentiation Activities of Levisticum officinale L. Extracts on Pathogenic Bacteria

Myracrondruon urundeuva Allemo: Chemical composition, antioxidant activity, antimicrobial activity and inotropic effect

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