Medicinal Plants as Potential Sources of Lead Compounds with Anti-Platelet and Anti-Coagulant Activ

Tk, Chua; Koh, HL

doi:10.2174/138955706777435751

Cited by 22 publications

(10 citation statements)

References 46 publications

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“…Much research has shown the importance of antithrombotic effect of polyphenol-rich plant extracts (Chua and Koh, 2006). In our previous in vitro studies, we found that incubation with polyphenol-rich extracts from chokeberry and grape seeds resulted in the changes of coagulation properties of human plasma (Bijak et al ., 2011).…”

Section: Discussionmentioning

confidence: 99%

Thrombin inhibitory activity of some polyphenolic compounds

et al. 2013

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Thrombin, also known as an active plasma coagulation factor II, belongs to the family of serine proteases and plays a crucial role in blood coagulation process. The process of thrombin generation is the central event of the hemostatic process and regulates blood coagulant activity. For this reason, thrombin inhibition is key to successful novel antithrombotic pharmacotherapy. The aim of our present study was to examine the effects of the well-known polyphenolic compounds on the activity of thrombin, by characterization of its interaction with selected polyphenols using different biochemical methods and biosensor BIAcore analyses. Only six compounds, cyanidin, quercetin, silybin, cyanin, (+)-catechin and (−)-epicatechin, of all examined in this study polyphenols caused the inhibition of thrombin amidolytic activity. But only three of the six compounds (cyanidin, quercetin and silybin) changed thrombin proteolytic activity. BIAcore analyses demonstrated that cyanidin and quercetin caused a strong response in the interaction with immobilized thrombin, while cyanin and (−)-epicatechin induced a low response. Lineweaver–Burk curves show that used polyphenol aglycones act as competitive thrombin inhibitors. Our results suggest that polyphenolic compounds might be potential structural bases and source to find and project nature-based, safe, orally bioavailable direct thrombin inhibitors.

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Section: Discussionmentioning

confidence: 99%

Thrombin inhibitory activity of some polyphenolic compounds

et al. 2013

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“…Although there are already osteoporosis medications and therapies, natural products still hold potential for treating osteoporosis, due to its higher acceptance by consumers. Fruit extracts is a major form of natural products, and a valuable and barely explored reservoir for plenty of disorders (Shen et al., ), including cancers (Chua & Koh, ; Greenwell & Rahman, ; Lampronti et al., ). Pepino ( Solanum muricatum , SM) is an evergreen plant native to South America (Huyskens‐Keil, Prono‐Widayat, Lüdders, & Schreiner, ), and is of close evolutionary relationship with tomato and eggplant.…”

Section: Introductionmentioning

confidence: 99%

“…Fruit extracts is a major N. Wang and L. Wang contributed equally to this work. form of natural products, and a valuable and barely explored reservoir for plenty of disorders (Shen et al, 2012), including cancers (Chua & Koh, 2006;Greenwell & Rahman, 2015;Lampronti et al, 2006). Pepino (Solanum muricatum, SM) is an evergreen plant native to South America (Huyskens-Keil, Prono-Widayat, Lüdders, & Schreiner, 2006), and is of close evolutionary relationship with tomato and eggplant.…”

Section: Introductionmentioning

confidence: 99%

Solanum muricatum Ameliorates the Symptoms of Osteogenesis Imperfecta In Vivo

Wang

et al. 2019

Journal of Food Science

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Pepino (Solanum muricatum), which is an evergreen plant native to South America, is well‐known for its effects in antioxidation, antidiabetic activity, anti‐inflammation, and antitumor activity. A previous study in our lab indicated that Solanum muricatum (SM) extract promoted osteogenic differentiation by upregulating Wnt and BMP signaling pathway in rat bone marrow stromal cells. The osteogenesis imperfecta (OI) mouse model was used in order to further discover the osteogenic properties of SM extract in the present research. We utilized microCT analysis to collect bone mass and microarchitectural parameters at vertebrae and at femur metaphysis in OI mice. Raman spectrometry was applied to identify change of bone mineral and matrix composition during SM treatment. Finally, collagen synthesis marker PINP and collagen degradation marker CTX were detected using enzyme immunoassay. SM extract could improve the bone mass and microarchitectural parameters both at vertebrae and at femur metaphysis. It also significantly increased the collagen content by promoting its biosynthesis and inhibiting its degradation. By using heterozygous Col1a1Jrt/+ mice as a model of OI, 6 weeks treatment of SM extract could significantly ameliorate the symptoms in OI mice. Thus, SM holds potential for developing new drugs of bone formation and bone remodeling.

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“…Therefore, researchers are at the challenging task to identify a novel anticoagulant agents with more therapeutic effects and without side effects. Hence, at present, lavish focus is being carried out on plant material to acts as a potent source for this kind of research 15,16 . Since there is a compelling scientific evidences that consumption of dietary anticoagulants with anti-coagulant properties reduces the risks of thrombotic disorders 17,18 .…”

Section: Introductionmentioning

confidence: 99%

A Preliminary Novel Findings on Invitro Anti-Coagulant Activity of Acalypha Indica Linn Leaf Extracts

Ashwini¹,

S²

2017

IJPCR

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The current study focuses on anticoagulant activity of leaf extract of Acalypha indica (A.indica) and to identify the active constituents present and responsible for the anti-coagulation activity. On sequential extraction of plant materials with petroleum ether, chloroform, ethyl acetate, n-butanol, ethanol and aqueous, crude extracts were obtained and screened for anti-coagulant activity. Anticoagulant activity of six different leaf extracts of A.indica was tested using prothrombin time (PT). In vitro anticoagulation assays were performed with different concentrations of the leaf extract on citrated plasma obtained from healthy volunteer donors. The different concentrations of crude extract tested in the present study were 0.062, 0.125, 0.25 and 0.5 gm/ml. The anti-coagulant activity of six extracts exhibited a concentration dependent activity. Among the six tested extracts, petroleum ether exhibited a highest activity by increased prothrombin time of 60min and 5 sec at 0.5gm/ml compared to positive and negative control. This is followed by aqueous, n-butanol, chloroform and ethyl acetate extract. It was also noted that ethanol extract showed no prolonged prothrombin time and it was within the normal level as compared to the control. Phytochemical screening of different extracts revealed the presence of steroids, terpenoids, tannin, phenols, flavonoids and alkaloids as secondary metabolites. From the results, for the first time it was highlighted that the A.indica leaf extracts affects the intrinsic pathway of coagulation cascade and thus prolongs the clotting time, hence this plant can be used in the management of blood clotting diseases.

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Medicinal Plants as Potential Sources of Lead Compounds with Anti-Platelet and Anti-Coagulant Activ

Cited by 22 publications

References 46 publications

Thrombin inhibitory activity of some polyphenolic compounds

Thrombin inhibitory activity of some polyphenolic compounds

Solanum muricatum Ameliorates the Symptoms of Osteogenesis Imperfecta In Vivo

A Preliminary Novel Findings on Invitro Anti-Coagulant Activity of Acalypha Indica Linn Leaf Extracts

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