Medium optimization for production of L-Glutaminase (EC 3.5.1.2) by Streptomyces griseus under submerged fermentation

Abstract

L-Glutaminase is widely distributed in microorganisms including bacteria, yeast and fungi. The enzyme mainly catalyzes the hydrolysis of γ-amido bond of l-glutamine. In this report medium optimization was conducted through one -factor -at -a -time approach for the submerged production of L-Glutaminase by Streptomyces griseus using different additional carbon, nitrogen, amino acids, mineral salts and was treated with different concentration sodium chloride. A significant influence of medium components (g/l) Galactose 10.0,Yeast extract 10.0,L-Glutamine 10.0,Magnesium sulphate 0.5, KH 2 PO 4 0.5 , K 2 HPO 4 0.5, NaCl 40 on L-Glutaminase production was noted. The applied methodology was validated using this optimized media, the enzyme activity 45 IU/ml in 48h of incubation was obtained.

Process Optimization of L-Glutaminase Production; a Tumour Inhibitor from Marine Endophytic Isolate Aspergillus sp. ALAA-2000

“…Overall, all carbon sources did not exhibited much significant increase in L-glutaminase production due to Dox modify medium free carbon relatively yielded a high amount of L-glutaminase (119.64 U/ml). On the contrary, the yield of L-glutaminase from S. griseus was increased to 26.3 U/ml by utilized the galactose as the carbon source [47]. Table 4 showed effect lysine, isoleucine, glycine, thiamine, arginine, treptophan, proline, glutamic acid, cysteine, andmethionine on L-glutaminase production.…”

“…L-glutamine at concentration of 2.5% supported the highest induction of L-glutaminase up 78.79 U/ml. The high yield of L-glutaminase (32.7 U/ml) produced by Streptomyces griseus when used L-glutamine as substrate due to L-glutamine is the specific inducers for L-glutaminase [47].…”

“…The optimum pH was determined by measuring enzymatic activity at 37°C using different buffers (0.1 M) with various pH values as: citrate-phosphate buffer (pH 3, 4, 5 and 6), phosphate buffer (pH 6-7), Tris-HCl buffer (pH 8-9) and glycine-NaOH buffer (pH 10-12). Optimum temperature was assayed by measuring activity with varying temperatures (27,37,47,57,67,77 and 87°C) at the optimum pH values. …”

Anticancer Glutaminase Production and Optimization Using Halotolerant Aspergillus Flavus Czcu-9, F1h

“…Howevere, L-glutaminase was reported to be synthesized via bacteria [3], fungi [4], yeast [5], actinomycetes [6].…”

Optimization of L-glutaminase synthesis by Aspergillus oryzae NRRL 32657 in submerged culture

“…Therefore, no change in pH level was detected. Suresh Kumar et al (2013) reported that the production of L-glutaminase from Serratia marcescens was maximized at 0.75% NaCl. On the other hand, Krishnakumar et al (2011) and Abdallah et al (2013) found that the maximal L-glutaminase activities produced by marine alkalophilic Streptomyces sp.-SBU1 and Streptomyces avermitilis were observed in a medium supplemented with 2 and 3% NaCl (w/v), respectively.…”