Medium optimization for the high yield production of single (+)-terrein by Aspergillus terreus strain PF26 derived from marine sponge Phakellia fusca

Yin, Ying; Gao, Qiang; Zhang, Fengli; Li, Zhiyong

doi:10.1016/j.procbio.2012.02.005

Cited by 21 publications

(14 citation statements)

References 29 publications

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“…We extracted and purified terrein from the fermentation broth of the marine sponge-derived fungus, Aspergillus terreus strain PF26, with high production efficiency and high quality as previously described (31,32). The structure of terrein is illustrated in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Terrein was separated from the fermentation broth of Aspergillus terreus strain PF-26 (31,32), and subjected to identification and quantification by high-performance liquid chromatography (HPLC) as previously described (31). The human ovarian epithelial cancer cell line, SKOV3, was purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

“…In our previous studies, we successfully isolated the (+)-terrein from the fermentation broth of the marine sponge-derived fungus, Aspergillus terreus strain PF26, with high production efficiency and high quality (31,32). In the present study, we aimed to investigate the anticancer effects of terrein isolated in this manner on a human epithelial ovarian cancer model.…”

Section: Introductionmentioning

confidence: 98%

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The marine-derived fungal metabolite, terrein, inhibits cell proliferation and induces cell cycle arrest in human ovarian cancer cells

Chen

Wang

Shen

et al. 2014

International Journal of Molecular Medicine

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Abstract. The difficulties faced in the effective treatment of ovarian cancer are multifactorial, but are mainly associated with relapse and drug resistance. Cancer stem-like cells have been reported to be an important contributor to these hindering factors. In this study, we aimed to investigate the anticancer activities of a bioactive fungal metabolite, namely terrein, against the human epithelial ovarian cancer cell line, SKOV3, primary human ovarian cancer cells and ovarian cancer stem-like cells. Terrein was separated and purified from the fermentation metabolites of the marine sponge-derived fungus, Aspergillus terreus strain PF26. Its anticancer activities against ovarian cancer cells were investigated by cell proliferation assay, cell migration assay, cell apoptosis and cell cycle assays. The ovarian cancer stem-like cells were enriched and cultured in a serum-free in vitro suspension system. Terrein inhibited the proliferation of the ovarian cancer cells by inducing G 2 /M phase cell cycle arrest. The underlying mechanisms involved the suppression of the expression of LIN28, an important marker gene of stemness in ovarian cancer stem cells. Of note, our study also demonstrated the ability of terrein to inhibit the proliferation of ovarian cancer stem-like cells, in which the expression of LIN28 was also downregulated. Our findings reveal that terrein (produced by fermention) may prove to be a promising drug candidate for the treatment of ovarian cancer by inhibiting the proliferation of cancer stem-like cells.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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The marine-derived fungal metabolite, terrein, inhibits cell proliferation and induces cell cycle arrest in human ovarian cancer cells

Chen

Wang

Shen

et al. 2014

International Journal of Molecular Medicine

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“…The 18S rRNA partial sequence placed the isolates IG 3 and DD1 within Aspergillus genera with 99% sequence homology with Aspergillus awamori (HQ393870) (Srinivasan et al , 2012). Similarly, isolate IG 4 showed maximum sequence similarity with Aspergillus terreus (JN831364) (Yin et al , 2012). Partial 18S rRNA sequences of all the isolates were submitted to NCBI Genbank under the following accession numbers: IG 1, JQ012799; IG 2, JQ012800; IG 3, JQ012801; IG 4, JQ012802, IG 5, JQ012803; DD1, JN624277; DD2, JN624278 and DD3, JN624279.…”

Section: Resultsmentioning

confidence: 99%

Isolation, morphological and molecular characterization of phytate-hydrolysing fungi by 18S rDNA sequence analysis

Gontia‐Mishra¹,

Deshmukh²,

Tripathi³

et al. 2013

Braz. J. Microbiol.

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Phytate is the primary storage form of phosphate in plants. Monogastric animals like poultry, pigs and fishes have very low or no phytase activities in their digestive tracts therefore, are incapable to efficiently utilize phytate phosphorus from the feed. Phytase from microbial sources are supplemented to feedstuff of these to increase the uptake of phytate phosphorus. In the present work efforts were made to isolate and characterize proficient phytase producing fungi from soil. Phytase producing fungi were isolated using phytate specific medium. Fungal isolates were selected according to their higher phytase activities. These isolates were further characterized and identified by morphological and microscopic analysis and confirmed by amplification of 18S rRNA gene, using specific primers. This gene was subsequently sequenced and phylogenetic affiliations were assigned. Fungal isolates were identified as various species of Aspergillus. Phytases from these fungi could be utilized as a feed additive in poultry and swine industries.

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“…But it could not be utilized by marine-derived Brevibacterium aureum MSA13 for producing lipopeptide biosurfactant (Kiran et al 2010). Maltose, taken as another important carbon source for producing OA by mutant P. oxalicum SCSIO 24-2, could be widely applied for producing different kinds of bioactive compounds (such as aspergiolide A, aspergillipeptides A-C, asteltoxin B, and (+)-terrain) by many marine-derived fungi (Cai et al 2012;Bao et al 2013;Yin et al 2012). In addition, according to the chemical structure of OA (Fig.…”

Section: Discussionmentioning

confidence: 99%

Enhanced production of a novel cytotoxic chromone oxalicumone A by marine-derived mutant Penicillium oxalicum SCSIO 24–2

Zhang

Bao

Zhong

et al. 2013

Appl Microbiol Biotechnol

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Many marine natural products hold great potential for the development of new and much needed drugs. However, the production of active metabolites by marine-derived microorganisms is usually very low, and large-scale culture has to be involved to meet the need of chemical structural modification and deep pharmacy study. In order to enhance the production of a novel cytotoxic sulfur-containing chromone oxalicumone A (OA), germinating spores of a marine-derived wild strain Penicillium oxalicum SCSGAF 0023 were mutated by microwave and ultraviolet light irradiation, which led to the obtainment of a mutant P. oxalicum SCSIO 24-2 that could produce fivefold increase in OA production (3.42±0.21 mg/l) as compared to the wild strain. This is the first report that germinating spores are applied in marine-derived Penicillium sp. mutating to enhance the production of OA. Further, Plackett-Burman design and central composite design were adopted to optimize the basic medium components for increasing OA production by the mutant SCSIO 24-2 in shake flasks. The results indicated that three medium components including mannitol, maltose, and L -cysteine had significant effects on OA production, and their concentrations were optimized as 36, 27.9, and 0.99 g/l, respectively. In the optimized medium, the OA production (18.31±0.27 mg/l) by mutant SCSIO 24-2 was 4.4-fold higher than that in the basic medium. These results of this work promise to improve the present production of OA and may be adopted to enhance other objective products' production by marine-derived fungi.

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Medium optimization for the high yield production of single (+)-terrein by Aspergillus terreus strain PF26 derived from marine sponge Phakellia fusca

Cited by 21 publications

References 29 publications

The marine-derived fungal metabolite, terrein, inhibits cell proliferation and induces cell cycle arrest in human ovarian cancer cells

The marine-derived fungal metabolite, terrein, inhibits cell proliferation and induces cell cycle arrest in human ovarian cancer cells

Isolation, morphological and molecular characterization of phytate-hydrolysing fungi by 18S rDNA sequence analysis

Enhanced production of a novel cytotoxic chromone oxalicumone A by marine-derived mutant Penicillium oxalicum SCSIO 24–2

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