Medium optimization using mathematical statistics for production of β-Carotene by Blakeslea trispora and fermenting process regulation

Yan, Zuowen; Wang, Changgao; Lin, Jianguo

doi:10.1007/s10068-013-0265-8

Cited by 17 publications

(11 citation statements)

References 23 publications

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“…The salt mix also had a positive linear effect on β-carotene concentration ( p < 0.05) in the present study. These results were not in agreement with the findings of Yan et al (2013) who reported non-significant linear effects of glucose, wheat bran extract and MgSO 4 percentages in the media as the variables in the RSM optimization study. They reported significant ( p < 0.05) quadratic effects of glucose and wheat bran extract.…”

Section: Resultscontrasting

confidence: 99%

“…They used RSM to determine the optimum values of linoleic acid, kerosene and antioxidant supplementation in molasses solution, and by using the optimum concentrations of the media supplements, they achieved a considerably high β-carotene concentration (790 mg/l). Yan et al (2013) also reported substantially high β-carotene concentration (643 mg/l) using the optimized media including glucose, wheat bran extract, MgSO 4 , soybean oil, thiamine, soy bean meal and KH 2 PO 4 . It is clearly seen from the results of the various studies that optimization using RSM works well in the determination of the optimal conditions for maximum β-carotene concentration.…”

Section: Resultsmentioning

confidence: 82%

“…Many authors have reported that the limitations of the classical optimization approach can be overcome by using statistics-based methods such as response surface methodology (RSM). RSM is a combined statistical method used for searching the optimum conditions and designing experiments, developing models and assessing the effects of factors (Choudhari and Singhal, 2008; Kalil et al , 2000; Yan et al , 2013), and it has been extensively used in the optimization of media for the production of microbial metabolites by various researchers (Choudhari and Singhal, 2008; Goksungur et al , 2002; Kalil et al , 2000; Nanou and Roukas, 2011; Yan et al , 2013).…”

Section: Introductionmentioning

confidence: 99%

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Optimizing β-carotene production byBlakeslea trisporausing bug damaged wheat

Velioglu

Sivri

2018

PRT

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Purpose β-carotene, found naturally in many foods, has been widely used as a colourant in various products. The aim of this study is to optimize the production of β-carotene by Blakeslea trispora using bug-damaged wheat as the main nitrogen and carbon source. Design/methodology/approach Response surface methodology (RSM) has been used to determine the optimum concentrations of media components for the maximum β-carotene production. β-carotene concentration was determined quantitively using a spectrophotometric method. Findings A full quadratic model fit the data best for β-carotene production with a high R2 value (0.999). Using optimized media increased the production of β-carotene by B. trispora DSM-2387 and B. trispora DSM-2388 to a higher level (250.407 mg/l) than the control medium (72.195 mg/l). Originality/value This study showed that bug-damaged wheat, an agro-industrial waste having low economical value, may be used as a valuable substrate for microbial production of β-carotene, a natural food colourant possessing high economical value.

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Section: Resultscontrasting

confidence: 99%

Section: Resultsmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

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Optimizing β-carotene production byBlakeslea trisporausing bug damaged wheat

Velioglu

Sivri

2018

PRT

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“…Most of the previous research on the fermentation process has been concerned with practical aspects of improving the overall carotenoid yield from producing cells by studying the effect of some variables as temperature, pH, nutrient concentration, mineral type, agitation speed, aeration, and light irradiation intensity . From an engineering point of view, the rate of the reaction in industrial processes is a principal factor affecting the cost effectiveness.…”

Section: Introductionmentioning

confidence: 99%

Kinetics of continuous production of β‐carotene in an airlift bioreactor

Taavoni

Habibi

Varmira

et al. 2017

Asia-Pacific J Chem Eng

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The focus in the present work was on the continuous β-carotene production from free fatty acids supplied by the soap stock as a sole carbon source for Rhodotorula rubra cells in an airlift bioreactor. The effects of dilution rate on the cell growth and β-carotene production were studied by both freely suspended-and immobilized-cell systems in the chemostat operations. The results showed that the immobilization of the cell on natural sponge of loofah significantly increased the optimum and maximum dilution rates from 0.0375 to 0.05 hr −1 and 0.055 to 0.150 hr −1 , respectively. The immobilization process has practical role in balancing of cell proliferation in the matrix and wash out the grown cells containing β-carotene from the bioreactor where β-carotene productivity was enhanced from 5.4 to 23.4 mg L −1 hr −1 using the immobilized cell technology.

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“…They added remarkable amount of Vitamin A acetate (1000 mg/L) to the culture medium which is an expensive component. Yan et al (2013) investigated the production of b-carotene in a complex medium and produced 669 mg/L b-carotene in optimum condition in shake flask. With considering glucose as main carbon source in their investigation, the productivity of b-carotene based on carbon source was 9.3 mg/g glucose which is approximately two times less than that of obtained in this study.…”

Section: Results For Screening Of Nitrogen Source Using Mixture Analymentioning

confidence: 99%

Screening of carbon and nitrogen sources using mixture analysis designs for carotenoid production by Blakeslea trispora

Shariati

Zare

Mirdamadi

2018

Food Sci Biotechnol

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The production of many secondary metabolites such as carotenoids is influenced by the type of carbon and nitrogen sources and C:N ratio applied in culture medium. The present study discusses the role of C:N ratio and screening of carbon and nitrogen sources using mixture analysis design in carotenoids production by Blakeslea trispora. The C:N ratios of 20, 40, and 60 with six nitrogen sources were evaluated. Results indicated that limitation of nitrogen source (C:N of 60) could improve carotenoids production. Six nitrogen and carbon sources were then screened using mixture analysis design. The most effective nitrogen and carbon sources were soybean powder and glucose, respectively. The productivity of carotenoids (983.8 ± 31.5 mg/L) based on consumed nitrogen and carbon source was 189.10 mg/g soybean powder and 19.66 mg/g glucose. Mixture analysis design indicated single carbon and nitrogen source were more effective than a mixture of sources for carotenoids production.

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Medium optimization using mathematical statistics for production of β-Carotene by Blakeslea trispora and fermenting process regulation

Cited by 17 publications

References 23 publications

Optimizing β-carotene production byBlakeslea trisporausing bug damaged wheat

Optimizing β-carotene production byBlakeslea trisporausing bug damaged wheat

Kinetics of continuous production of β‐carotene in an airlift bioreactor

Screening of carbon and nitrogen sources using mixture analysis designs for carotenoid production by Blakeslea trispora

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