Screening of carbon and nitrogen sources using mixture analysis designs for carotenoid production by Blakeslea trispora

Shariati, Sepideh; Zare, Davood; Mirdamadi, Saeed

doi:10.1007/s10068-018-0484-0

Cited by 17 publications

(8 citation statements)

References 27 publications

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“… 45 , 46 At S 3 level, N 3 treatment had the highest content of Car. This may be due to that nitrogen application increases the contents of rate limiting enzymes (PSY, PDS, and ZDS) 47 , 48 in the process of Car synthesis, thus increasing the content of Car. Therefore, the application of moderate rate of N could effectively scavenge excess ROS through enhancing the activities of POD, SOD, and CAT in rapeseed seedlings at different levels of soil salinity.…”

Section: Discussionmentioning

confidence: 99%

Nitrogen application alleviates salt stress by enhancing osmotic balance, ROS scavenging, and photosynthesis of rapeseed seedlings (Brassica napus)

Tian

Wang

et al. 2022

Plant Signaling & Behavior

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Nitrogen application could alleviate salt stress on crops, but the specific physiological mechanism is still unclear. Therefore, in this study, a pot experiment was conducted to explore the effects of different application rates of nitrogen (0, 0.15, 0.30, and 0.45 g·kg −1 ) on the growth parameters, osmotic adjustment, reactive oxygen species scavenging, and photosynthesis of rapeseed seedlings planted in the soils with different concentrations of sodium chloride (1.5, 3.5, 5.5, and 7.5 g·kg −1 ). The results showed that nitrogen could alleviate the inhibition of salt on rapeseed growth, and improve the antioxidant enzyme activities and the contents of non-enzymatic substances, K + , soluble protein (SP), soluble sugar (SS), and proline. Besides, there was a significant correlation between the indexes of active oxygen scavenging system, osmoregulation system, and photosynthesis. Therefore, applying appropriate amount of nitrogen can promote the growth and development of rapeseed seedlings under salt stress, accelerate the scavenging of reactive oxygen species, maintain osmotic balance, and promote photosynthesis. This study will improve our understanding on the mechanism by which nitrogen application alleviates salt stress to crops.

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Section: Discussionmentioning

confidence: 99%

Nitrogen application alleviates salt stress by enhancing osmotic balance, ROS scavenging, and photosynthesis of rapeseed seedlings (Brassica napus)

Tian

Wang

et al. 2022

Plant Signaling & Behavior

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“…51 Since the presence of a high amount of carbon in the environment suppresses respiration and mitochondrial activities, the amount of ATP decreases during development and it is thought that working above a certain carbon concentration is not necessary for the production of caroteoid, since this event indirectly suppresses the production of enzymes necessary for the production of secondary metabolites. [52][53][54] In the current study, it seems that there is an optimum production of carotenoid within a certain range of carbon amount. It may be evaluated due to carbon catabolite repression.…”

Section: Total Carotenoid μG=lmentioning

confidence: 51%

Optimization of carotenoid production by Umbelopsis ramanniana

Kizilay

Küçükçetin

Demir

2023

Biotechnology Progress

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Umbelopsis ramanniana was investigated to increase carotenoid production. Nine different carbon sources and six different nitrogen sources were evaluated for the maximum carotenoid production. The most effective nitrogen and carbon sources were KNO3 and lactose, respectively. Then, the optimization of medium components for enhancement of carotenoid production by Umbelopsis ramanniana was achieved using Plackett–Burman design. Box–Behnken response surface methodology was applied to further optimize carotenoid and biomass production. Carbon to nitrogen ratio, lactose concentration, and shaking speed were studied as variables in Box–Behnken design. The optimum conditions for carotenoid and biomass production were determined as 32.42 g/L of lactose concentration, 20:1 of carbon to nitrogen ratio, and shaking speed of 130 rpm. The maximum carotenoid and biomass production under optimized conditions were 1141 μg/L (β‐carotene‐Eq) and 13.14 g/L, respectively. When compared to the control fermentation, carotenoid, and biomass production were increased by about 2 and 1.3 folds, respectively.

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“…Another most critical parameter that influences the development of microbial lipids and carotenoid is the mass balance of carbon and nitrogen sources, in particular cultivation medium improvement [ 33 ]. The carbon source and C/N ratio are associated with metabolite and biomass production and affect the secondary metabolite yield [ 34 ]. Therefore, various strategies are often employed to find the optimal C/N ratio for enhanced bioactive compounds.…”

Section: Resultsmentioning

confidence: 99%

Redirecting Metabolic Flux towards the Mevalonate Pathway for Enhanced β‐Carotene Production in M. circinelloides CBS 277.49

Naz

Nazir

Nosheen

et al. 2020

BioMed Research International

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Carotenoids produced by microbial sources are of industrial and medicinal importance due to their antioxidant and anticancer properties. In the current study, optimization of β-carotene production in M. circinelloides strain 277.49 was achieved using response surface methodology (RSM). Cerulenin and ketoconazole were used to inhibit fatty acids and the sterol biosynthesis pathway, respectively, in order to enhance β-carotene production by diverting metabolic pool towards the mevalonate pathway. All three variables used in screening experiments were found to be significant for the production of β-carotene. The synergistic effect of the C/N ratio, cerulenin, and ketoconazole was further evaluated and optimized for superior β-carotene production using central composite design of RSM. Our results found that the synergistic combination of C/N ratios, cerulenin, and ketoconazole at different concentrations affected the β-carotene productions significantly. The optimal production medium (std. order 11) composed of C/N 25, 10 μg/mL cerulenin, and 150 mg/L ketoconazole, producing maximum β-carotene of 4.26 mg/L (0.43 mg/g) which was 157% greater in comparison to unoptimized medium (1.68 mg/L, 0.17 mg/g). So, it was concluded that metabolic flux had been successfully redirected towards the mevalonate pathway for enhanced β-carotene production in CBS 277.49.

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Screening of carbon and nitrogen sources using mixture analysis designs for carotenoid production by Blakeslea trispora

Cited by 17 publications

References 27 publications

Nitrogen application alleviates salt stress by enhancing osmotic balance, ROS scavenging, and photosynthesis of rapeseed seedlings (Brassica napus)

Nitrogen application alleviates salt stress by enhancing osmotic balance, ROS scavenging, and photosynthesis of rapeseed seedlings (Brassica napus)

Optimization of carotenoid production by Umbelopsis ramanniana

Redirecting Metabolic Flux towards the Mevalonate Pathway for Enhanced β‐Carotene Production in M. circinelloides CBS 277.49

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