Medullasin enhances human natural killer cell activity.

Aoki, Yosuke; Sumiya, M; Oshimi, Kazuo

doi:10.1172/jci110561

Cited by 25 publications

(5 citation statements)

References 30 publications

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“…Conversely, the possibility that proteins released from neutrophil-derived granules might instead positively regulate NK cell functions was put forward by Aoki et al [28], who showed that neutrophil elastase can enhance NK cell activities by PBL toward K562 or Molt-4 cells. Subsequently, lactoferrin was shown to enhance the NK-mediated cytotoxicity and IL-2-induced LAK activity by PBLs [33].…”

Section: Figure 2 Schematic Depiction Of the Interactions Between Mumentioning

confidence: 99%

The defensive alliance between neutrophils and NK cells as a novel arm of innate immunity

Costantini

Cassatella

2010

Journal of Leukocyte Biology

121

102

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The immune system is equipped with a plethora of mechanisms that protect the host from the harmful effects of environmental insults. However, the traditional "hierarchical" view of the immune response, in which innate, "nonspecific" cells are first recruited to the site of damage, before the highly "specific", adaptive immune response develops, has been questioned recently. First, the innate response is much more specific than recognized previously: indeed, each cell of the innate system is not only endowed with an ever-expanding array of germ-line-encoded receptors, which differentiate between distinct insults, but also is modulated continuously by other leukocytes that concomitantly interact with and respond to that particular insult. The other reason is that the cells of the innate system are instrumental for the adaptive system to accomplish its function, as they can also modulate the activity of lymphocytes reciprocally during the entire course of the immune response. This complex pattern of interactions is illustrated by recent advances on the functions of PMNs, clearly showing that unexpectedly, these cells also contribute to the regulation of the host immune response by crosstalk with innate and adaptive leukocytes, including NK cells. Herein, given the peculiar role of neutrophils and NK cells in inflammation, clearance of pathogens/viral-infected cells, and cancer immunosurveillance, we summarize the current knowledge about the mechanisms whereby neutrophils and NK cells interact and regulate the activities of one another, as well as discuss their potential implications involved in the pathogenesis of chronic, inflammatory pathologies, infections, and tumors.

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Section: Figure 2 Schematic Depiction Of the Interactions Between Mumentioning

confidence: 99%

The defensive alliance between neutrophils and NK cells as a novel arm of innate immunity

Costantini

Cassatella

2010

Journal of Leukocyte Biology

121

102

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“… 20) Medullasin also plays important roles in the regulation of both immonity and nonspecific self defense mechanism (biophylaxis). It enhances human natural killer cell activity by causing the maturation of immature natural killer cells, 21) , 22) induces activated killer cells from CD16 positive cells which injure every malignant cell, 23) , 24) and increases DNA synthesis of human lymphocytes. 25) Medullasin also potentiates cytostatic activity and superoxide production of monocytes.…”

Section: Discussionmentioning

confidence: 99%

Azurocidin induces elastinolytic activity of medullasin

Aoki

2006

Proceedings of the Japan Academy. Ser. B: Physical and Biologic

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Addition of azurocidin, a protein in granulocytes similar to serine proteases but has no protease activity because of replacement of the active serine residue by glycine, to the incubation mixture containing medullasin induced elastinolytic activity of medullasin. Both medullasin and human leukocyte elastase were already shown to have negligible elastinolytic activity (Aoki, Y. et al. J. Biochem. 114, 122, 1993). Elastinolytic activity of medullasin was induced dose-dependently by the addition of azurocidin. Medullasin activity determined by using apo-ornithine transaminase or casein as substrates or that by N-methoxy-succinyl-(Ala)2-Pro-Val-p-nitroanilide as substrate remained unchanged when azurocidin was added to the tube containing medullasin. Therefore, azurocidin is considered to cause an appearance of elastinolytic activity of medullasin without affecting the protease activity of it.

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“…Medullasin has been shown to play an important role in biophylaxis. It induces in¯ammation by injuring endothelial cells in vessels (4), increases human natural killer cell activity (11), and activates both monocyte and lymphocyte functions (5). Therefore, it is possible that medullasin plays certain roles in causing clinical symptoms in multiple sclerosis.…”

Section: Discussionmentioning

confidence: 99%