Meiotic analysis of interspecific hybrids between Capsicum frutescens and Capsicum chinense

Moreira, Nádia Fernandes; Pereira, Telma Nair Santana; Martins, Kellen Coutinho

doi:10.1590/1984-70332017v17n2n23

Cited by 6 publications

(6 citation statements)

References 23 publications

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“…While 2n = 2x = 24 species have symmetrical karyotypes, low DNA amount and simple heterochromatic banding patterns, 2n = 2x = 26 species have asymmetric karyotypes and higher DNA and heterochromatin contents. Different inter-and intra-specific cytotypes are common and indicate very active processes of chromosome diversification in that group (Moscone et al 2007, Teodoro-Pardo et al 2007, Scaldaferro et al 2013, Moreira et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Heterochromatin distribution and histone modification patterns of H4K5 acetylation and H3S10 phosphorylation in Capsicum L.

Martins

Perón

Lopes

et al. 2018

Crop Breed. Appl. Biotechnol.

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Section: Introductionmentioning

confidence: 99%

Heterochromatin distribution and histone modification patterns of H4K5 acetylation and H3S10 phosphorylation in Capsicum L.

Martins

Perón

Lopes

et al. 2018

Crop Breed. Appl. Biotechnol.

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“…In our study we found meiotic indices of 90% which represent cytological stability. The main irregular post-meiotic products such as triads may be caused by the occurrence of irregularity spindle bers (Souza and Pereira 2011) as well as errors in cytoplasmic division during meiosis I and II (Moreira et al 2017). In addition, micronuclei can also be formed by cytomixis (transfer of material from one cell to another) (Diegues et al 2015), but this event was not visualized in this study.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, micronuclei can also be formed by cytomixis (transfer of material from one cell to another) (Diegues et al 2015), but this event was not visualized in this study. Post-meiotic products with irregularities can originate from gametes with an extra chromosomal set, non-degraded gametes, or generate aneuploid gametes (Souza et al 2003), or unbalanced PG (Moreira et al 2017) or sterile (Souza et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Meiosis and male fertility in F1 interspecific hybrids (Passiflora vitifolia vs. Passiflora hatschbachii)

Souza

Silva

et al. 2022

Euphytica

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Interspeci c hybrids can be studied using methodologies in which the male gamete with high reproduction potential, viability, and fertility is prioritized. Passi ora species, with lush, showy, and exotic colors, have great potential for the ornamental plant market. In addition, arti cial Passi ora hybrids were developed without many di culties because of weak reproductive barriers. Thus, meiotic and post-meiotic behaviors were analyzed with 2% acetic carmine staining. Con rmation of interspeci c hybridization was performed using SSR markers and GISH technique was used to detect genomic differentiation. The pollen viability of the parental and hybrids genotypes was tested using Alexander solution, uorescein diacetate and in vitro germination tests were performed using culture medium. The meiotic behavior was regular and displayed haploid number n = 9 with nine bivalent (II) chromosomal, pairing in 90% of the cells in diakinesis. There was a signi cant difference (p < 0.05) in terminal and interstitial chiasma frequencies. Meiotic irregularities observed were as follows: early and/or delayed chromosomes, disorientation of spindle bers, transverse spindles, tripolar spindles, and asynchrony; and consequently irregular post-meiotic products were observed: monads, dyads, triads, and polyads. GISH was used in the interspeci c hybrids and pairing between homeologous chromosomes, and bivalent and tetravalent formation were observed. From this study, we could conclude that hybrid genotypes are fertile and pollen grains are viable and can be used in breeding programs. We also hypothesize that interspeci c hybrid genotypes of Passi ora can be obtained with regular meiosis, which could be viable and fertile.

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“…Ploidy level was determined from the gametic chromosome number and pollen mother cells (PMC) analysis. During the summer of 2010, young inflorescences of two individuals randomly chosen from each accession were collected and fixed in Carnoy 3:1 (ethanol: acetic acid) for 24 hours, at room temperature, and stored in ethanol 70% under refrigeration (Pereira et al 2014, Moreira et al 2017). Slides were prepared by isolating the anthers, squashing, and staining with 1% propionic carmine (Pagliarini et al 2002, Simioni andVale 2009).…”

Section: Methodsmentioning

confidence: 99%

New wild diploids in Paspalum notatum Flügge (Poaceae): potential accessions for use in breeding.

Fachinetto

Dall'Agnol

Schifino-Wittmann

et al. 2018

Crop Breed. Appl. Biotechnol.

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Paspalum notatum is a polymorphic species with two accepted varieties, according to the ploidy level. The ploidy level is closely related to the reproductive mode of this species. Usually, diploid cytotypes have sexual reproduction, and tetraploid cytotypes have an apomictic reproduction. Apomixis limits genetic recombination, hindering the development of new cultivars. The identification of sexual plants represents many possibilities in breeding programs. This study aimed to analyze the chromosome number in P. notatum accessions under agronomic evaluation. Ploidy level was determined in 25 accessions. Two ploidy levels were detected, resulting in 21 tetraploid and four diploid accessions. The four diploid accessions originate from Argentina and Uruguay, in a region close to the origin of Pensacola, which is a diploid sexual cultivar of P. notatum. These new diploid accessions may be useful in the development of breeding programs of Paspalum species.

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Meiotic analysis of interspecific hybrids between Capsicum frutescens and Capsicum chinense

Abstract: Abstract:The aim of this study was to observe the genetic relationship between C. frutescens (UENF 1636)

Cited by 6 publications

References 23 publications

Heterochromatin distribution and histone modification patterns of H4K5 acetylation and H3S10 phosphorylation in Capsicum L.

Heterochromatin distribution and histone modification patterns of H4K5 acetylation and H3S10 phosphorylation in Capsicum L.

Meiosis and male fertility in F1 interspecific hybrids (Passiflora vitifolia vs. Passiflora hatschbachii)

New wild diploids in Paspalum notatum Flügge (Poaceae): potential accessions for use in breeding.

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