Meiotic chromosome pairing in Actinidia chinensis var. deliciosa

Mertten, D.; Tsang, G.K.; Manako, K.I.; McNeilage, M.A.; Datson, P. M.

doi:10.1007/s10709-012-9693-2

Cited by 20 publications

(8 citation statements)

References 26 publications

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“…Non-preferential chromosome pairing occurs during meiosis when chromosomes pair with more than one potential homologue partner. Both natural and induced polyploids in kiwifruit can form multivalent chromosome formations (Mertten et al 2012 ; Wu et al 2014 ). This finding suggests an adjustment of NRM to polyploidy, and double reduction (ω) should be considered in the breeding strategy of Actinidia spp.…”

Section: Introductionmentioning

confidence: 99%

Implementation of different relationship estimate methodologies in breeding value prediction in kiwiberry (Actinidia arguta)

Mertten,

Baldwin,

Cheng

et al. 2023

Mol Breeding

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In dioecious crops such as Actinidia arguta (kiwiberries), some of the main challenges when breeding for fruit characteristics are the selection of potential male parents and the long juvenile period. Currently, breeding values of male parents are estimated through progeny tests, which makes the breeding of new kiwiberry cultivars time-consuming and costly. The application of best linear unbiased prediction (BLUP) would allow direct estimation of sex-related traits and speed up kiwiberry breeding. In this study, we used a linear mixed model approach to estimate narrow sense heritability for one vine-related trait and five fruit-related traits for two incomplete factorial crossing designs. We obtained BLUPs for all genotypes, taking into consideration whether the relationship was pedigree-based or marker-based. Owing to the high cost of genome sequencing, it is important to understand the effects of different sources of relationship matrices on estimating breeding values across a breeding population. Because of the increasing implementation of genomic selection in crop breeding, we compared the effects of incorporating different sources of information in building relationship matrices and ploidy levels on the accuracy of BLUPs’ heritability and predictive ability. As kiwiberries are autotetraploids, multivalent chromosome formation and occasionally double reduction can occur during meiosis, and this can affect the accuracy of prediction. This study innovates the breeding programme of autotetraploid kiwiberries. We demonstrate that the accuracy of BLUPs of male siblings, without phenotypic observations, strongly improved when a tetraploid marker-based relationship matrix was used rather than parental BLUPs and female siblings with phenotypic observations.

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Section: Introductionmentioning

confidence: 99%

Implementation of different relationship estimate methodologies in breeding value prediction in kiwiberry (Actinidia arguta)

Mertten,

Baldwin,

Cheng

et al. 2023

Mol Breeding

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“…Crowhurst and Gardner (1991) suggested that A. deliciosa may be an allopolyploid species with diploid A. chinense as one parent; A. deliciosa has a family of repeat sequences that is absent in diploid A. chinensis , but the other parental species is (are) unknown. However, the absence of preferential chromosome pairing suggests that A. deliciosa is an autopolyploid species in terms of chromosome behaviour (Mertten et al., 2012). Analyses of isozyme polymorphisms also support an autopolyploid origin (Testolin & Ferguso, 1997).…”

Section: Introductionmentioning

confidence: 99%

Chromosome‐level genome of putative autohexaploid Actinidia deliciosa provides insights into polyploidisation and evolution

Liu,

Zhou,

Cheng

et al. 2023

The Plant Journal

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SUMMARYActinidia (‘Mihoutao’ in Chinese) includes species with complex ploidy, among which diploid Actinidia chinensis and hexaploid Actinidia deliciosa are economically and nutritionally important fruit crops. Actinidia deliciosa has been proposed to be an autohexaploid (2n = 174) with diploid A. chinensis (2n = 58) as the putative parent. A CCS‐based assembly anchored to a high‐resolution linkage map provided a chromosome‐resolved genome for hexaploid A. deliciosa yielded a 3.91‐Gb assembly of 174 pseudochromosomes comprising 29 homologous groups with 6 members each, which contain 39 854 genes with an average of 4.57 alleles per gene. Here we provide evidence that much of the hexaploid genome matches diploid A. chinensis; 95.5% of homologous gene pairs exhibited >90% similarity. However, intragenome and intergenome comparisons of synteny indicate chromosomal changes. Our data, therefore, indicate that if A. deliciosa is an autoploid, chromosomal rearrangement occurred following autohexaploidy. A highly diversified pattern of gene expression and a history of rapid population expansion after polyploidisation likely facilitated the adaptation and niche differentiation of A. deliciosa in nature. The allele‐defined hexaploid genome of A. deliciosa provides new genomic resources to accelerate crop improvement and to understand polyploid genome evolution.

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“…So, by elucidating the genetic variations in triploid loquats after their formation, more details could be provided for elucidating the above phenomenon (heterosis) in triploid loquats as well as triploid loquat breeding. However, it is quite hard for us to carry out relative research on the genetic variations in triploid loquats due to the fact that the origin and evolution of loquats are still unclear, which makes it impossible for us to conduct a study using natural triploid loquats, whereas cross-breeding provides us with an effective solution to this problem since tetraploid loquats have limited fertility, based on reported studies, and the egg cells of tetraploid loquats produced via megaspore-mother-cell meiosis contain a certain number of viable diploid egg cells, which could be fertilized by the diploid father [ 39 ]. Based on this, Wang (2008) successfully obtained several triploid loquats via cross-breeding (4× × 2×) and analyzed their genetic variations after their formation using the AFLP marker [ 40 ].…”

Section: Introductionmentioning

confidence: 99%

Photosynthetic Efficiency and Glyco-Metabolism Changes in Artificial Triploid Loquats Contribute to Heterosis Manifestation

Wang

MeiYan

et al. 2022

IJMS

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Previous studies indicated that extensive genetic variations could be generated due to polyploidy, which is considered to be closely associated with the manifestation of polyploid heterosis. Our previous studies confirmed that triploid loquats demonstrated significant heterosis, other than the ploidy effect, but the underlying mechanisms are largely unknown. This study aimed to overcome the narrow genetic distance of loquats, increase the genetic variation level of triploid loquats, and systematically illuminate the heterosis mechanisms of triploid loquats derived from two cross combinations. Here, inter-simple sequence repeats (ISSRs) and simple sequence repeats (SSRs) were adopted for evaluating the genetic diversity, and transcriptome sequencing (RNA-Seq) was performed to investigate gene expression as well as pathway changes in the triploids. We found that extensive genetic variations were produced during the formation of triploid loquats. The polymorphism ratios of ISSRs and SSRs were 43.75% and 19.32%, respectively, and almost all their markers had a PIC value higher than 0.5, suggesting that both ISSRs and SSRs could work well in loquat assisted breeding. Furthermore, our results revealed that by broadening the genetic distance between the parents, genetic variations in triploids could be promoted. Additionally, RNA-Seq results suggested that numerous genes differentially expressed between the triploids and parents were screened out. Moreover, KEGG analyses revealed that “photosynthetic efficiency” and “glyco-metabolism” were significantly changed in triploid loquats compared with the parents, which was consistent with the results of physiological indicator analyses, leaf micro-structure observations, and qRT-PCR validation. Collectively, our results suggested that extensive genetic variations occurred in the triploids and that the changes in the “photosynthetic efficiency” as well as “glyco-metabolism” of triploids might have further resulted in heterosis manifestation in the triploid loquats.

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Meiotic chromosome pairing in Actinidia chinensis var. deliciosa

Cited by 20 publications

References 26 publications

Implementation of different relationship estimate methodologies in breeding value prediction in kiwiberry (Actinidia arguta)

Implementation of different relationship estimate methodologies in breeding value prediction in kiwiberry (Actinidia arguta)

Chromosome‐level genome of putative autohexaploid Actinidia deliciosa provides insights into polyploidisation and evolution

Photosynthetic Efficiency and Glyco-Metabolism Changes in Artificial Triploid Loquats Contribute to Heterosis Manifestation

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