Meiotic regulation of the Ndc80 complex composition and function

Abstract

This review describes the current models for how the subunit abundance of the Ndc80 complex, a key kinetochore component, is regulated in budding yeast and metazoan meiosis. The past decades of kinetochore research have established the Ndc80 complex to be a key microtubule interactor and a central hub for regulating chromosome segregation. Recent studies further demonstrate that Ndc80 is the limiting kinetochore subunit that dictates the timing of kinetochore activation in budding yeast meiosis. Here, we discuss the molecular circuits that regulate Ndc80 protein synthesis and degradation in budding yeast meiosis and compare the findings with those from metazoans. We envision the regulatory principles discovered in budding yeast to be conserved in metazoans, thereby providing guidance into future investigations on kinetochore regulation in human health and disease.

Integrated bioinformatics analysis and experimental validation reveals hub genes of rheumatoid arthritis

“…Loss of BRD2 and BRD4 leads to downregulation of the expression of genes that are critically involved in the pathogenesis and progression of RA (28). Upon activation or high expression of four hub genes identified in the present study (DLGAP5, KIF20A, MELK and NDC80), growth, proliferation and migration of cells, including pro-inflammatory cells and macrophages, is promoted (29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39). Under the action of these genes, pro-inflammatory cells and macrophages then migrate to the lesion site and the inflammatory cells begin to release a number of inflammatory factors (such as IL-12 and TNF-α), which causes systemic inflammation and macrophage infiltration (29,40,41).…”

“…NDC80/Hec1, a subunit of the kinetochore complex (also called the NDC80 complex), constitutes and stabilizes microtubule-kinetochore attachment during the segregation of mitotic chromosomes, and plays an important role in mitosis and cell proliferation (38). NDC80 upregulation is also an important biomarker for cancer; high levels of NDC80 have been reported to indicate a poor prognosis in various types of cancer, such as gastric cancer, ovarian cancer and blood cancer (39). Although DLGAP5, KIF20A, MELK and NDC80 have been studied separately in different tumors, to the best of our knowledge, there is still a lack of evidence for the correlation between DLGAP5, KIF20A, MELK and NDC80 and RA development.…”

Role of CENPF and NDC80 in the rehabilitation nursing of hepatocellular carcinoma and cirrhosis: An observational study

“…This ensures that each daughter cell contains a complete set of chromosomes and is vital for chromosome separation and spindle formation. [ 24 , 25 ] The expression and function of the NDC80 complex are regulated by the cell cycle, particularly during mitosis, to ensure its alignment with different stages of the cell cycle. [ 26 ] Aberrations in mitosis and chromosome instability are associated with cancer development.…”

Patterns of gene expression in ovaries of sexual vs. asexual lineages of a freshwater snail

“…One of these transcripts was annotated as kinetochore protein spc24, an essential component of the NDC80 complex, which is necessary for chromosome segregation (McCleland et al, 2003). In yeast, the regulation of NDC80 subunits is integral for meiosis I to occur (reviewed in (Chen and Ünal, 2021). Of potential relevance to the P. antipodarum system is that a lineage of baker's yeast with a unique non-synonymous mutation in NDC80 was also found to have a particularly high incidence of polyploidy (83% vs. 34% of all 144 strained analyzed) (Zhu et al, 2016).…”