Melanin-Decolorizing Activity of Antioxidant Enzymes, Glutathione Peroxidase, Thiol Peroxidase, and Catalase

Jeon, Gyeongchan; Kim, Chinhan; Cho, Uk Min; Hwang, Ee Taek; Hwang, Han Sung; Min, Jiho

doi:10.1007/s12033-020-00292-6

Cited by 21 publications

(14 citation statements)

References 17 publications

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“…GPx also aids in catalyzing H 2 O 2 and organic hydroperoxides [45,46]. CAT and GPx enzymes help cells get rid of free radicals [47]. Consistent with the results of these studies, our findings revealed the effect of exposure to H 2 at different time intervals on boosting the activity of antioxidant enzymes such as CAT and GPx after stress induction by H 2 O 2 and LPS.…”

Section: Discussionsupporting

confidence: 90%

Antioxidant Properties of Hydrogen Gas Attenuates Oxidative Stress in Airway Epithelial Cells

et al. 2021

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Oxidative stress plays a crucial role in the development of airway diseases. Recently, hydrogen (H2) gas has been explored for its antioxidant properties. This study investigated the role of H2 gas in oxidative stress-induced alveolar and bronchial airway injury, where A549 and NCI-H292 cells were stimulated with hydrogen peroxide (H2O2) and lipopolysaccharide (LPS) in vitro. Results show that time-dependent administration of 2% H2 gas recovered the cells from oxidative stress. Various indicators including reactive oxygen species (ROS), nitric oxide (NO), antioxidant enzymes (catalase, glutathione peroxidase), intracellular calcium, and mitogen-activated protein kinase (MAPK) signaling pathway were examined to analyze the redox profile. The viability of A549 and NCI-H292 cells and the activity of antioxidant enzymes were reduced following induction by H2O2 and LPS but were later recovered using H2 gas. Additionally, the levels of oxidative stress markers, including ROS and NO, were elevated upon induction but were attenuated after treatment with H2 gas. Furthermore, H2 gas suppressed oxidative stress-induced MAPK activation and maintained calcium homeostasis. This study suggests that H2 gas can rescue airway epithelial cells from H2O2 and LPS-induced oxidative stress and may be a potential intervention for airway diseases.

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Section: Discussionsupporting

confidence: 90%

Antioxidant Properties of Hydrogen Gas Attenuates Oxidative Stress in Airway Epithelial Cells

et al. 2021

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“…Superoxide dismutase (SOD), the first line of defense system against ROS, can catalyze the conversion from O 2 • − into H 2 O 2 , which can subsequently be eliminated by peroxidases such as catalase (CAT) and glutathione peroxidase (GSH-Px) while CAT plays a predominant role in decomposing H 2 O 2 into harmless molecules [ 3 ]. The increase in antioxidant enzyme activity can reduce the oxidative stress of living cells [ 48 ]. In the present study, the activities of antioxidant enzymes (SOD, CAT, and GSH-Px) were measured to evaluating the protective effects of GDPs against H 2 O 2 -induced oxidative damage.…”

Section: Resultsmentioning

confidence: 99%

Enzymatic Degradation of Gracilariopsis lemaneiformis Polysaccharide and the Antioxidant Activity of Its Degradation Products

Tian

Zhang

et al. 2021

Marine Drugs

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Gracilariopsis lemaneiformis polysaccharides (GLP) were degraded using pectinase, glucoamylase, cellulase, xylanase, and β-dextranase into low-molecular-weight polysaccharides, namely, GPP, GGP, GCP, GXP, and GDP, respectively, and their antioxidant capacities were investigated. The degraded GLPs showed higher antioxidant activities than natural GLP, and GDP exhibited the highest antioxidant activity. After the optimization of degradation conditions through single-factor and orthogonal optimization experiments, four polysaccharide fractions (GDP1, GDP2, GDP3, and GDP4) with high antioxidant abilities (hydroxyl radical scavenging activity, DPPH radical scavenging activity, reduction capacity, and total antioxidant capacity) were obtained. Their cytoprotective activities against H2O2-induced oxidative damage in human fetal lung fibroblast 1 (HFL1) cells were examined. Results suggested that GDP pretreatment can significantly improve cell viability, reduce reactive oxygen species and malonaldehyde levels, improve antioxidant enzyme activity and mitochondria membrane potential, and alleviate oxidative damage in HFL1 cells. Thus, the enzyme degradation of GLP with β-dextranase can significantly improve its antioxidant activity, and GDP might be a suitable source of natural antioxidants.

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“…Several hydrolytic enzymes are present in LOE, which is derived from egg white. Recently, we have reported that activated form of peroxidase, which is an antioxidant enzyme present in lysosomes, results in the bleaching of melanin [13,16]. Moreover, lysophosphatidic acid of eggs is known to inhibit melanin synthesis by inhibiting the protein expression of MITF and tyrosinase in α-MSH-induced Mel-Ab cell line [28][29][30].…”

Section: Discussionmentioning

confidence: 99%

The inhibitory effect of egg white lysosome extract (LOE) on melanogenesis through ERK and MITF regulation

Park

Hwang

2022

JABC

Self Cite

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Lysosome organelle extract (LOE) was derived from egg whites. The lysosome is an intracellular organelle that contains several hydrolysis enzymes. Previous studies have reported that LOE performs important functions, such as melanin decolorization and anti-melanin production in B16F10 melanoma cells. However, its principal molecular and cellular mechanisms have not been elucidated till date. In non-cytotoxic conditions, LOE significantly inhibited α-MSH induced melanin synthesis of murine B16F10 cells. The anti-melanogenic activity of LOE was mediated by suppressing the mRNA expression of tyrosinase enzyme, tyrosinase related protein-1/2 (TRP-1/2), and microphthalmiaassociated transcription factor (MITF) genes. By performing western blot analysis, we found that LOE significantly attenuated melanogenesis. In this case, LOE helped in increasing extracellular receptor kinase (ERK) phosphorylation in α-MSH induced B16F10 cells. Furthermore, MITF is found to be a key regulatory transcription factor in melanin synthesis; it was down-regulated by LOE through ERK phosphorylation. In this experiment, PD98059 (MEK inhibitor) was used to check whether LOE directly regulated the activity of ERK. Although LOE exerted inhibitory effect on melanin synthesis, we could not observe this effect in PD98059-treated α-MSH induced B16F10. These results strongly indicate that LOE is related to ERK activation and MITF degradation in anti-skin pigmentation. Hence, LOE should be utilized as a whitening agent of skin in the near future.

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Melanin-Decolorizing Activity of Antioxidant Enzymes, Glutathione Peroxidase, Thiol Peroxidase, and Catalase

Cited by 21 publications

References 17 publications

Antioxidant Properties of Hydrogen Gas Attenuates Oxidative Stress in Airway Epithelial Cells

Antioxidant Properties of Hydrogen Gas Attenuates Oxidative Stress in Airway Epithelial Cells

Enzymatic Degradation of Gracilariopsis lemaneiformis Polysaccharide and the Antioxidant Activity of Its Degradation Products

The inhibitory effect of egg white lysosome extract (LOE) on melanogenesis through ERK and MITF regulation

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