Melanogenesis in normal and phenylthiourea‐treated eggs and larvae of <i>Sarotherodon aureus</i>

Lanzing, W. J. R.

doi:10.1111/j.1095-8649.1984.tb04798.x

Cited by 6 publications

(3 citation statements)

References 14 publications

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“…In particular, zebrafish researchers use phenylthiourea to suppress pigmentation in developing embryos due to its ability to interfere with tyrosinase activity (25,26,34). Similarly, thiourea reduces pigmentation in coral trout grouper, tilapia, European eel, and Japanese flounder (33,35,36,27). Similar effects were also reported in zebrafish (31) treated with methimazole.…”

Section: Discussionmentioning

confidence: 97%

Type 2 Iodothyronine Deiodinase Is Essential for Thyroid Hormone-Dependent Embryonic Development and Pigmentation in Zebrafish

et al. 2008

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Despite the known importance of thyroid hormones (THs) in vertebrate growth and development, the role of tissue-specific TH activation in early embryogenesis remains unclear. We therefore examined the function of type 2 iodothyronine deiodinase (D2), one of the two tissue-specific enzymes catalyzing the conversion of T4 to T3, in developing zebrafish embryos (Danio rerio). Microinjection of early embryos with antisense oligonucleotides targeting either the D2 translation start site or the splice junction between the first exon and intron induced delays in development and pigmentation, as determined through the measurement of otic vesicle length, head-trunk angle, and pigmentation index at 31 h after fertilization. The antisense-induced delays in developmental progression and pigmentation were reversible through treatment with T3, suggesting that these phenotypic effects may be due to the depletion of intracellular T3 levels. Additional evidence for this hypothesis was provided by quantitative RT-PCR analysis of TH receptor-beta expression in D2 knockdown embryos, revealing a significant down-regulation of this T3-induced transcript that could be reversed by T3 treatment. Tyrosinase expression was also down-regulated in D2 knockdown embryos to a greater degree than could be predicted by the observed delay in developmental progression, suggesting that reduced D2 activity and resultant low intracellular T3 availability may directly influence pigmentation in zebrafish. These data indicate that TH activation by D2 is essential for embryonic development and pigmentation in zebrafish.

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Section: Discussionmentioning

confidence: 97%

Type 2 Iodothyronine Deiodinase Is Essential for Thyroid Hormone-Dependent Embryonic Development and Pigmentation in Zebrafish

et al. 2008

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“…Such inhibitory effects of antithyroid drugs on larval growth and development have been reported in several fishes and cited as evidence for a role of thyroid hormones in early development of fish (Dales and Hoar 1954;Honma and Murakawa 1955;Lanzing 1984;Munro 1984). Phenylthiourea and thiocarbamides are known goitrogens inhibiting the iodination of tyrosine which is essential for the production of thyroid hormones (Mountcastle 1980;Ganong 1981).…”

Section: T 4 and T~ Therapy In Ptc-treated Larvaementioning

confidence: 94%

Role of thyroid hormones in tilapia larvae (Oreochromis mossambicus): I. Effects of the hormones and an antithyroid drug on yolk absorption, growth and development

Reddy

Lam

1992

Fish Physiol Biochem

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Treatment of one-day old yolksac larvae of tilapia (Oreochromis mossambicus) by immersion in 0.05 ppm T4 or 0.01 ppm T3 significantly accelerated the differentiation and growth of all the fins, particularly pectoral and tail fins. Both the treatments also significantly accelerated yolk absorption and transition to free-swimming activity in the larvae. The treatments also significantly accelerated the growth of the larvae, with T3 at 0.01 ppm having a greater effect than T4 at 0.05 ppm. The yolk conversion efficiency was found not to be significantly affected by the hormone treatments but the treated larvae exhibited an increased heart beat, suggesting metabolic stimulation by the hormones.On the other hand, yolk absorption and free-swimming activity were significantly delayed in tilapia larvae immersed in 25 ppm solution of an antithyroid drug, phenylthiocarbamide (PTC). PTC also retarded the growth of the larvae. T4 (0.05 and 0.10 ppm) or T3 (0.01 and 0.02 ppm) therapy removed the PTC-inhibition,albeit not completely, suggesting that thyroid hormones are involved in the larval growth and development of tilapia.

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“…oval bodies with the pigment melanin (Rogers et al 1997;Van Den Bossche et al 2006). The major function of melanosomes is to protect the DNA in the spinous-layer cells from harmful ultraviolet (UV) radiation (Lanzing 1984). Environmental factors and changes occurring within the body activate the melanophores, their increasing activity being manifested as intracellular responses.…”

Section: Introductionmentioning

confidence: 99%

Magnetic field effect on melanophores of the European whitefish Coregonus lavaretus (Linnaeus, 1758) and vendace Coregonus albula (Linnaeus, 1758) (Salmonidae) during early embryogenesis

Brysiewicz

Formicki

Tański

et al. 2017

The European Zoological Journal

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Melanogenesis in normal and phenylthiourea‐treated eggs and larvae of Sarotherodon aureus

Cited by 6 publications

References 14 publications

Type 2 Iodothyronine Deiodinase Is Essential for Thyroid Hormone-Dependent Embryonic Development and Pigmentation in Zebrafish

Type 2 Iodothyronine Deiodinase Is Essential for Thyroid Hormone-Dependent Embryonic Development and Pigmentation in Zebrafish

Role of thyroid hormones in tilapia larvae (Oreochromis mossambicus): I. Effects of the hormones and an antithyroid drug on yolk absorption, growth and development

Magnetic field effect on melanophores of the European whitefish Coregonus lavaretus (Linnaeus, 1758) and vendace Coregonus albula (Linnaeus, 1758) (Salmonidae) during early embryogenesis

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