Melanogenesis Inhibition Effects of Nemopilema nomurai Hydrolized Extracts

Abstract

This study was aimed to investigate skin whitening effects of Nomura's jellyfish (Nemopilema nomurai) hydrolyzed extracts (NHE). The extracts were prepared through the hydrolysis of N. nomurai using commercial proteolytic enzymes such as Protamex, Alcalase, Flavourzyme and Neutrase with optimum pHs (pH 5-8) at 55 ℃. Their whitening activities were examined from the inhibition of melanin synthesis using B16-F1 cell lines. Among the examined samples, Neutrase-treated extract (N-NHE) showed the most significant inhibition effect on melanin synthesis by 89.9% at a concentration of 100 µg/mL. This sample decreased the expression of tyrosinase and TRP-1 (tyrosinase related protein-1) proteins as well. These results suggested the potential application of NHE as whitening ingredients in cosmetic preparation.

Anti-Inflammatory and Whitening Effect of the Lyophilized Powder of Oriental Plant Extracts Fermented with Streptococcus thermophilus

“…멜라닌은 표피층 내부에 자리잡은 멜라닌형성세포 (melanocyte) 내 소기관인 멜라노좀(melanosome)에서 아미노산인 tyrosine을 출발물질로 하여 생합성되며 멜라노좀 내에 있는 tyrosinase, tyrosinase-related protein 1 (TRP1)과 dopachrome tautomerase (DCT)라고도 불리는 tyrosinase-related protein 2 (TRP2) 등의 여러 가지 효소의 복합 작용에 의해 생성된다 [7,8]. 따라서 이러한 효소들은 멜라닌 합성을 조절하는 피부 미백 제를 개발하기 위한 주요 타깃으로서 연구되어져 왔다 [9][10][11][12].…”