Melanoma Growth Stimulatory Activity in Primary Malignant Melanoma: Prognostic Significance

Middleman, Benton R; Friedman, Michaël; Lawson, David H.; DeRose, Patricia B.; Cohen, Cynthia

doi:10.1038/modpathol.3880559

Cited by 8 publications

(7 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, higher CXCL1 levels were associated with a reduction in disease-specific survival. Similar survival results have been reported in gastric cancer [24], breast cancer [25] and melanoma [26,27]. These findings support a role for CXCL1 in bladder tumor initiation and progression, and further studies are underway to better define the influence of CXCL1 in BCa.…”

Section: Discussionsupporting

confidence: 85%

Chemokine (C-X-C) ligand 1 (CXCL1) protein expression is increased in aggressive bladder cancers

et al. 2013

View full text Add to dashboard Cite

BackgroundChemokines, including chemokine (C-X-C motif) ligand 1 (CXCL1), may regulate tumor epithelial-stromal interactions that facilitate tumor growth and invasion. Studies have linked CXCL1 expression to gastric, colon and skin cancers, but limited studies to date have described CXCL1 protein expression in human bladder cancer (BCa).MethodsCXCL1 protein expression was examined in 152 bladder tissue specimens (142 BCa) by immunohistochemical staining. The expression of CXCL1 was scored by assigning a combined score based on the proportion of cells staining and intensity of staining. CXCL1 expression patterns were correlated with clinicopathological features and follow-up data.ResultsCXCL1 protein expression was present in cancerous tissues, but was entirely absent in benign tissue. CXCL1 combined immunostaining score was significantly higher in high-grade tumors relative to low-grade tumors (p = 0.012). Similarly, CXCL1 combined immunostaining score was higher in high stage tumors (T2-T4) than in low stage tumors (Ta-T1) (p < 0.0001). An increase in the combined immunostaining score of CXCL1 was also associated with reduced disease-specific survival.ConclusionTo date, this is the largest study describing increased CXCL1 protein expression in more aggressive phenotypes in human BCa. Further studies are warranted to define the role CXCL1 plays in bladder carcinogenesis and progression.

show abstract

Section: Discussionsupporting

confidence: 85%

Chemokine (C-X-C) ligand 1 (CXCL1) protein expression is increased in aggressive bladder cancers

et al. 2013

View full text Add to dashboard Cite

show abstract

“…anoma with a predicted median false-discovery rate of 2.3 (Table 3). Up-regulated in this gene set were genes known to play an important role in melanoma progression, including SPP1 (osteopontin) (12,13), and CXCL1 (melanoma growth-stimulating activity) (14), and RAB32 (15). Conversely, genes with potential tumor suppressor activity such as WIF1 (16), ECM2 (17), and SLIT3 (18,19) were down-regulated.…”

Section: Resultsmentioning

confidence: 99%

The gene expression signatures of melanoma progression

Haqq

Nosrati

Sudilovsky

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

405

371

View full text Add to dashboard Cite

Because of the paucity of available tissue, little information has previously been available regarding the gene expression profiles of primary melanomas. To understand the molecular basis of melanoma progression, we compared the gene expression profiles of a series of nevi, primary melanomas, and melanoma metastases. We found that metastatic melanomas exhibit two dichotomous patterns of gene expression, which unexpectedly reflect gene expression differences already apparent in comparing laser-capture microdissected radial and vertical phases of a large primary melanoma. Unsupervised hierarchical clustering accurately separated nevi and primary melanomas. Multiclass significance analysis of microarrays comparing normal skin, nevi, primary melanomas, and the two types of metastatic melanoma identified 2,602 transcripts that significantly correlated with sample class. These results suggest that melanoma pathogenesis can be understood as a series of distinct molecular events. The gene expression signatures identified here provide the basis for developing new diagnostics and targeting therapies for patients with malignant melanoma.bioinformatics ͉ human ͉ microarray ͉ metastasis ͉ laser capture I n the current staging system for cutaneous melanoma, vertical thickness of the primary tumor is the dominant prognostic factor, belying the fact that a subset of thin tumors metastasize, whereas some thick tumors do not undergo metastasis (1). The original melanoma tumor progression model is characterized by an initial radial growth phase, encompassing in situ and minimally invasive tumors (2). This phase is followed by the development of vertical growth phase, which has been postulated to be the first point at which the tumor gains metastatic capacity. However, metastasis occurs, although with decreased frequency, in patients whose primary melanoma pathology exhibits only a radial growth pattern (3). Previous transcriptome analysis in melanoma defined a cluster of genes expressed in a majority of metastatic melanomas (4); however, this cluster was not related to radial or vertical growth, and precursor nevi (moles) and primary melanomas were not examined. Likewise, mutations in B-RAF occur commonly in both nevi (5) and melanoma (6), and, thus, do not distinguish progressive stages in melanoma progression. In this study, we used cDNA expression array profiling to characterize the global patterns of transcript modulation that underlie the various phases in the known tumor progression pathway of melanoma. MethodsStudy Subjects. Samples from melanoma patients and nevus volunteers presenting to the Melanoma Center were obtained with informed consent under a protocol approved by the UCSF Institutional Review Board. After biopsy, all samples were frozen in OCT freezing medium over dry ice. Subsequently, samples were processed for hematoxylin͞eosin staining and confirmed by pathologic review. Only samples comprised of Ͼ95% tumor cells were analyzed.

show abstract

“…The ability to stimulate new vessel formation (angiogenesis) is an important factor in tumor progression in melanoma and other tumors. 4,5,32,33 Angiogenesis is mediated by various proteins, among them the proangiogenic factors we examined: VEGF, TGF-b, aVb3 integrin, COX-2, and CD44. In contrast, tumor suppressor proteins (eg, maspin) are of interest in that they may be markers for tumors with less invasive potential.…”

Section: Discussionmentioning

confidence: 99%

Maspin expression, angiogenesis, prognostic parameters, and outcome in malignant melanoma

Chua

Setzer

Gunasekaran

et al. 2009

Journal of the American Academy of Dermatology

Self Cite

View full text Add to dashboard Cite

Melanoma Growth Stimulatory Activity in Primary Malignant Melanoma: Prognostic Significance

Cited by 8 publications

References 20 publications

Chemokine (C-X-C) ligand 1 (CXCL1) protein expression is increased in aggressive bladder cancers

Chemokine (C-X-C) ligand 1 (CXCL1) protein expression is increased in aggressive bladder cancers

The gene expression signatures of melanoma progression

Maspin expression, angiogenesis, prognostic parameters, and outcome in malignant melanoma

Contact Info

Product

Resources

About