Melatonin added to freezing diluent improves canine (Bulldog) sperm cryosurvival

Martinez-Rodriguez, Julio A; Carbajal, Francisco J.; Martinez-De-Anda, Rocio; Alcantar-Rodriguez, Alicia; Medrano, Alfredo Soriano

doi:10.1530/raf-20-0022

Cited by 13 publications

(12 citation statements)

References 24 publications

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“…Unfortunately, the type of melatonin solvent was not reported in the study. In another study on Bulldogs [ 10 ], the authors tested the effect of melatonin in the freezing medium on sperm cryo-survival. Similar to us, they used DMSO as the melatonin solvent.…”

Section: Discussionmentioning

confidence: 99%

“…The protective effects of melatonin supplementation of the cryopreservation media on the porcine, bovine, ovine, equine and, human frozen-thawed spermatozoa have been previously reported in several independent studies [ 9 ]. Canine frozen-thawed ejaculated [ 10 ] or epididymal [ 11 ] spermatozoa have also benefited from in vitro supplementation of the melatonin in the freezing extender. However, multiple studies show that the extent of the protective effects is species-specific and highly dependent on the melatonin concentration.…”

Section: Introductionmentioning

confidence: 99%

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Supplementation of melatonin to cooling and freezing extenders improves canine spermatozoa quality measures

et al. 2022

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Background Sperm freezing and cold storage are the two most common assisted reproductive technologies in the canine breeding industry. The freeze-thawing process causes significant detrimental changes in both sperm cell structure and function. Previous research has confirmed that excessive accumulation of un-scavenged free radicals (oxidative stress) plays an important role in the cryopreservation-induced damage to sperm cells. Also, the gradual accumulation of the free radicals during cold storage leads to a decline in the sperm quality markers. Melatonin is an endogenous neurohormone synthesized from tryptophan amino acid by pineal glands. Besides its several well-known physiologic roles, melatonin has a significant antioxidant potential through direct free radical scavenging properties. Therefore, the current study was designed to evaluate the potential in vitro protective properties of melatonin (0.5, 1, and 2 mM) on canine sperm cells after freezing or during long-term cold storage (9 days, 5 °C) on most important sperm in vitro fertility markers. Results Melatonin at 0.5, 1- or 2-mM concentrations could preserve significantly higher sperm total motility after 4 days of cold storage. However, only the 1- and 2 mM melatonin concentrations could result in better TM and PM values after 7 days of cold storage. Furthermore, melatonin supplementation could preserve higher sperm viability and acrosome integrity after 7 days of storage. Also, it could have significant protective effects on the cooled sperm DNA integrity. In the freezing section of the current research, melatonin at either 1- or 2-mM concentrations could not improve the sperm post-thaw TM and PM, whereas they improved sperm DNA integrity. Also, the post-thaw plasma membrane functional integrity and sperm velocity parameters were not affected by the treatment. Although DMSO (Dimethyl Sulfoxide) as the melatonin solvent could reduce the level of sperm lipid peroxidation and even improve the post-thaw sperm DNA integrity compared to the negative control, it reduced the post-thaw sperm progressive motility. However, the negative effects were reversed by concurrent melatonin supplementation at 1- and 2-mM concentrations. Conclusion The addition of 1- or 2-mM melatonin to the canine sperm freezing and cooling media could improve sperm motility, viability, acrosome, and DNA integrity.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Supplementation of melatonin to cooling and freezing extenders improves canine spermatozoa quality measures

et al. 2022

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“…Since its discovery as a component of cooling extender, egg yolk has been widely used in mammalian sperm cryopreservation to protect sperm from initial cold shock [ 12 , 13 ]. Moreover, many studies have verified the benefits of egg yolk-based extenders for canine semen cryopreservation [ 14 , 15 ]. However, it has been stated that the use of egg yolk interferes with sperm evaluation and the presence of particulate material in the extender may reduce fertility [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

The Use of κ-Carrageenan in Egg Yolk Free Extender Improves the Efficiency of Canine Semen Cryopreservation

Kim

Almubarak

Nabeel

et al. 2021

Animals

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κ-Carrageenan is a plant polysaccharide derived from red seaweeds reported to possess potential medicinal and antioxidants activities. The present study aimed to identify the cryoprotective effects of κ-carrageenan on the quality of frozen-thawed canine semen. Twenty-eight ejaculates were collected and diluted in a Tris egg-yolk-free extender supplemented with various concentrations of κ-carrageenan (0.0%, 0.1%, 0.2%, 0.3%, and 0.5%). The addition of κ-carrageenan to the extender at a 0.2% concentration induced a significant increase in the total motility (TM) and the rapid progressive motility (RPM) of canine sperm. Among the experimental groups, the highest percentage of sperms with intact acrosomes was found in the 0.5% κ-carrageenan group (p < 0.05). Apoptosis levels were significantly lower in the 0.1% and 0.2% κ-carrageenan treatment. Moreover, sperm in the κ-carrageenan supplemented group showed a significantly higher expression of antiapoptotic (Bcl-2) and lower expression of NADPH oxidase (NOX5), spermine synthase (SMS), and spermine oxidase (SMOX) genes than those in the control group. In conclusion, the addition of κ-carrageenan to the freezing extender improved the overall efficiency of frozen-thawed dog spermatozoa.

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“…The addition of 1–2 mM of melatonin could also improve the sperm motility of canine sperm ( 17 ). In canine sperm, 0.002 and 0.0035 mol/L of melatonin received good results in the conservation of mitochondrial activity ( 16 ). The effect of melatonin on the acrosome in dogs is controversial.…”

Section: Discussionmentioning

confidence: 99%

“…On the contrary, the negative effect of excess melatonin concentration on spermatozoa has been described ( 15 ). Recently, several studies have observed the effect of melatonin supplementation on canine semen quality using several melatonin concentrations, e.g., 0.0005–0.0035 mol/L ( 16 ) and 0.5–2 mM ( 17 ). However, no reference to potential seasonal effects was made.…”

Section: Introductionmentioning

confidence: 99%

Photoperiod and Melatonin Supplementation: Variable Effects on the Quality of Chilled Dog Semen

Mitjana

Ausejo

Mendoza³

et al. 2022

Front. Vet. Sci.

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The addition of melatonin in seminal extenders due to its antioxidant properties and its beneficial role in sperm preservation has been previously described, especially in seasonal species. The aim of this study was to study a potential seasonal effect based on photoperiod duration when adding a physiological concentration of melatonin in the canine ejaculate. A total of 24 ejaculates were obtained from 10 healthy dogs during the increasing photoperiod (from December 21 to June 21), whereas 12 ejaculates were collected from five healthy individuals during the decreasing photoperiod (from June 22 to December 20). Each ejaculate was separated into two aliquots, and one of them remained as a control, whereas melatonin (100 pM) was added to the other one (C and M treatment groups, respectively). Diluted semen was refrigerated at 5°C. On days 0, 1, 2, 3, and 6, sperm motility analyses were performed using a CASA system and hypoosmotic swelling test (HOST), osmotic resistance test (ORT), and flow cytometry analysis. No effect of melatonin on motility was detected in either photoperiod. Negative effects of melatonin were found for acrosomal defects, apoptosis, and viability in the decreasing photoperiod. The addition of melatonin to sperm in the decreasing photoperiod could create such a high level that it would cause the described negative effects. We found a beneficial effect of melatonin in the increasing photoperiod on acrosomal defects and apoptosis during 0–6 days. Melatonin treatment also increased viability in the short term (days 1 and 2) for both photoperiods. Also, melatonin can provide certain beneficial effects on mitochondrial activity in the medium term (days 2 and 3) in the decreasing photoperiod.

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Melatonin added to freezing diluent improves canine (Bulldog) sperm cryosurvival

Cited by 13 publications

References 24 publications

Supplementation of melatonin to cooling and freezing extenders improves canine spermatozoa quality measures

Supplementation of melatonin to cooling and freezing extenders improves canine spermatozoa quality measures

The Use of κ-Carrageenan in Egg Yolk Free Extender Improves the Efficiency of Canine Semen Cryopreservation

Photoperiod and Melatonin Supplementation: Variable Effects on the Quality of Chilled Dog Semen

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