Melatonin counteracts BMP-6 regulation of steroidogenesis by rat granulosa cells

Nakamura, Eri; Otsuka, Fumio; Terasaka, Tomohiro; Inagaki, Kenichi; Hosoya, Takeshi; Tsukamoto-Yamauchi, Naoko; Toma, Kishio; Makino, Hírofumi

doi:10.1016/j.jsbmb.2014.04.003

Cited by 22 publications

(21 citation statements)

References 30 publications

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“…As mentioned above, the mechanism by which melatonin suppresses BMP activity in granulosa cells was found to be the induction of inhibitory smad‐6 expression in the granulosa cells . As an antagonistic effect of melatonin on BMP action was shown in ovarian steroidogenesis, similar regulatory interactions between melatonin and BMPs are seen in adrenocortical steroidogenesis (Figure ).…”

Section: Melatonin and Bone Morphogenetic Proteins In Other Endocrinementioning

confidence: 69%

“…Given that both melatonin and BMP‐6 are involved in progesterone synthesis and the luteinization process in the ovary, a functional interaction between melatonin and BMP signaling is thought to exist in the granulosa cells (Figure ). The results of experiments using rat granulosa cells showed that the melatonin treatment did not affect steroidogenetic activities, such as estradiol or progesterone production by granulosa cells. However, of note, the inhibitory effect of BMP‐6 on FSH‐induced progesterone production was reversed in the presence of melatonin actions (Figure ) .…”

Section: Interaction Of Melatonin and Bone Morphogenetic Proteins In mentioning

confidence: 94%

“…The results of experiments using rat granulosa cells showed that the melatonin treatment did not affect steroidogenetic activities, such as estradiol or progesterone production by granulosa cells. However, of note, the inhibitory effect of BMP‐6 on FSH‐induced progesterone production was reversed in the presence of melatonin actions (Figure ) . In accordance with its effect on progesterone synthesis by granulosa cells, melatonin reversed the inhibitory effects of BMP‐6 on cyclic adenosine monophosphate synthesis, as well as the mRNA expression of steroidogenic factors and enzymes, including steroidogenic acute regulatory protein, P450 steroid side‐chain cleavage enzyme, and 3β‐hydroxysteroid dehydrogenase, induced by FSH stimulation (Figure ) …”

Section: Interaction Of Melatonin and Bone Morphogenetic Proteins In mentioning

confidence: 94%

“…Recently, the authors reported an interesting activity of melatonin in the control of BMP receptor (BMPR) signaling and ovarian steroidogenesis by regulating inhibitory smad expression . The existence of a molecular interaction between melatonin and BMPR‐to‐smad signaling also seems to be applicable to various ligands other than melatonin.…”

Section: Introductionmentioning

confidence: 99%

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Modulation of bone morphogenetic protein activity by melatonin in ovarian steroidogenesis

Otsuka

2018

Reprod Medicine & Biology

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BackgroundMelatonin regulates circadian and seasonal rhythms and the activities of hormones and cytokines that are expressed in various tissues, including the ovary, in which melatonin receptors are expressed. In the ovary, follicular growth occurs as a result of complex interactions between pituitary gonadotropins and autocrine and paracrine factors, including bone morphogenetic proteins (BMPs) that are expressed in the ovary.MethodsThe effects of melatonin and BMPs on steroidogenesis were examined by using the primary cultures of rat granulosa cells.Main findings (Results)It was shown that melatonin has antagonistic effects on BMP‐6 actions in the granulosa cells, suggesting that melatonin is likely to contribute to balancing the biological activity of endogenous BMPs that maintain progesterone production and luteinization in the growing follicles. Similar interactions between melatonin and BMP–smad signaling also were shown in the mechanism of controlling ovarian steroidogenesis by other ligands.ConclusionA new role of melatonin in the regulation of endocrine homeostasis in relation to BMP activity is introduced in this review.

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Section: Melatonin and Bone Morphogenetic Proteins In Other Endocrinementioning

confidence: 69%

Section: Interaction Of Melatonin and Bone Morphogenetic Proteins In mentioning

confidence: 94%

Section: Interaction Of Melatonin and Bone Morphogenetic Proteins In mentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Modulation of bone morphogenetic protein activity by melatonin in ovarian steroidogenesis

Otsuka

2018

Reprod Medicine & Biology

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“…We have also reported an inhibitory effect of melatonin on ACTH production by pituitary corticotrope cells, in which MT1 and BMP-4 actions were mutually enhanced [24]. In the ovary, melatonin was shown to suppress BMP-6-induced SMAD1/5/8 signaling in granulosa cells [37]. In adrenocortical cells, melatonin facilitated aldosterone production induced by ACTH combined with activin via the cAMP-PKA pathway [13].…”

Section: Regarding the Influence Of Melatonin On Regulation Of Systemmentioning

confidence: 76%

Melatonin regulates catecholamine biosynthesis by modulating bone morphogenetic protein and glucocorticoid actions

Komatsubara

Hara

Hosoya

et al. 2017

The Journal of Steroid Biochemistry and Molecular Biology

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Simultaneous release of melatonin and methylprednisolone from an injectable in situ self‐crosslinked hydrogel/microparticle system for cartilage tissue engineering

Naghizadeh

Karkhaneh

Khojasteh

2018

J Biomedical Materials Res

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Recently, injectable hydrogel/microparticle systems have so considered for tissue engineering and regenerative medicine. In this study, we produced an injectable in situ self-crosslinked hydrogel/microparticle system for simultaneous dual drug delivery. First, melatonin conjugated chitosan microparticle loaded with methylprednisolone (MCC-MP) microparticle was fabricated by the covalent linkage of melatonin to chitosan by N-hydroxysuccinimide (NHS)1-Ethyl-3-(3-dimethylamino propyl)-carbodiimide (EDC) followed by an ionic gelation of MCC and MP using tripolyphosphate (TPP). Second, the hydrogel was prepared by the connection between the aldehyde group of alginate oxide (AD) and the amine group belonging to carboxymethyl chitosan (CMC) via Schiff base reaction. Finally, microparticle was incorporated into the AD-CMC hydrogel to produce a hydrogel/microparticle system. Hydrogel/microparticle was assessed by many techniques including microscopy, spectroscopy, particle size measurements, mechanical analysis, injectability, rheological analyses to ascertain hydrogel/microparticle properties. The biological assays of mesenchymal stem cells (MSCs) culture, 3-(4,5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), acridine orange/propidium iodide (AO/PI), and 4, 6-diamidino-2-phenylindole (DAPI) to assess cell viability and dimethylmethylene blue (DMMB) to evaluate proteoglycan content were done. The release profiles of melatonin and MP showed acceptable release after 60 and 20 days, respectively. The hydrogel/microparticle system has the ability to sustain cells alive. A higher rate of proteoglycan content was observed in hydrogel/microparticle as compared with hydrogel. With appropriate biocompatibility and adequate properties, this system can be a proper alternative for cartilage tissue engineering. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1932-1940, 2018.

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Melatonin counteracts BMP-6 regulation of steroidogenesis by rat granulosa cells

Cited by 22 publications

References 30 publications

Modulation of bone morphogenetic protein activity by melatonin in ovarian steroidogenesis

Modulation of bone morphogenetic protein activity by melatonin in ovarian steroidogenesis

Melatonin regulates catecholamine biosynthesis by modulating bone morphogenetic protein and glucocorticoid actions

Simultaneous release of melatonin and methylprednisolone from an injectable in situ self‐crosslinked hydrogel/microparticle system for cartilage tissue engineering

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