Melatonin modulates adiponectin expression on murine colitis with sleep deprivation

Kim, Tae Kyun; Park, Sung Chul; Baik, Haing-Woon; Jun, Jin Hyun; Kim, Min Jung; Sull, Jae Woong; Sung, Ho Joong; Choi, Jin Woo; Chung, Sung Pil; Gye, Myung Chan; Lim, Ju Yeon; Kim, Jun Bong; Kim, Seong Hwan

doi:10.3748/wjg.v22.i33.7559

Cited by 13 publications

(10 citation statements)

References 37 publications

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“…Melatonin and DSS + melatonin groups received melatonin (10 mg/kg/day) by i.p. from days 1 to 8, while Control and DSS + vehicle groups were treated with 0.25% EtOH/PBS i.p based on our previous studies using DSS-induced colitis to detect rapid response 8,13,62 . All mice were sacrificed on day 9, and colon length was measured.…”

Section: Methodsmentioning

confidence: 99%

Melatonin controls microbiota in colitis by goblet cell differentiation and antimicrobial peptide production through Toll-like receptor 4 signalling

Kim

Son

et al. 2020

Sci Rep

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Microbial dysbiosis has long been postulated to be associated with the pathogenesis of inflammatory bowel disease (IBD). Although evidence supporting the anti-colitic effects of melatonin have been accumulating, it is not clear how melatonin affects the microbiota. Herein, we investigated the effects of melatonin on the microbiome in colitis and identified involvement of Toll-like receptor (TLR) 4 signalling in the effects. Melatonin improved dextran sulfate sodium (DSS)-induced colitis and reverted microbial dysbiosis in wild-type (WT) mice but not in TLR4 knockout (KO) mice. Induction of goblet cells was observed with melatonin administration, which was accompanied by suppression of Il1b and Il17a and induction of melatonin receptor and Reg3β, an antimicrobial peptide (AMP) against Gram-negative bacteria. In vitro, melatonin treatment of HT-29 intestinal epithelial cells promotes mucin and wound healing and inhibits growth of Escherichia coli. Herein, we showed that melatonin significantly increases goblet cells, Reg3β, and the ratio of Firmicutes to Bacteriodetes by suppressing Gram-negative bacteria through TLR4 signalling. Our study suggests that sensing of bacteria through TLR4 and regulation of bacteria through altered goblet cells and AMPs is involved in the anti-colitic effects of melatonin. Melatonin may have use in therapeutics for iBD.

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Section: Methodsmentioning

confidence: 99%

Melatonin controls microbiota in colitis by goblet cell differentiation and antimicrobial peptide production through Toll-like receptor 4 signalling

Kim

Son

et al. 2020

Sci Rep

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“…Many previous reports have shown that melatonin has operative anti-inflammatory effects in experimental colitis. [6][7][8] Recently, it has been reported that melatonin can modulate specific gut microbiota activity and abundances. 9 It has been shown that melatonin can influence the swarming and motility of human intestinal bacteria, especially Enterobacter aerogenes.…”

Section: Introductionmentioning

confidence: 99%

Melatonin in the colon modulates intestinal microbiota in response to stress and sleep deprivation

et al. 2020

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Background/Aims: Stress is closely related to the deterioration of digestive disease. Melatonin has potent anti-inflammatory properties. The objective of this study was to determine the effect of water stress (WS) and sleep deprivation (SD) on intestinal microbiota and roles of melatonin in stressful condition. Methods: We used C57BL/6 mice and specially designed water bath for stress and SD for 10 days. We measured melatonin concentrations in serum, feces, and colon tissues by high-performance liquid chromatography. Genomic DNA was extracted from feces and amplified using primers targeting V3 to V4 regions of bacterial 16S ribosomal RNA genes. Results: Compared to the control, melatonin concentration was lower in the WS and SD. Fecal concentration was 0.132 pg/mL in control, 0.062 pg/mL in WS, and 0.068 pg/mL in SD. In colon tissue, it was 0.45 pg/mL in control, 0.007 pg/mL in WS, and 0.03 pg/mL in SD. After melatonin treatment, melatonin concentrations in feces and colon tissue were recovered to the level of control. Metagenomic analysis of microbiota showed abundance in colitogenic microbiota in WS and SD. Melatonin injection attenuated this harmful effect. WS and SD showed decreased Lactobacillales and increased Erysipelotrichales and Enterobacteriales. Melatonin treatment increased Akkermansia muciniphila and Lactobacillus and decreased Bacteroides massiliensis and Erysipelotrichaceae. Conclusions: This study showed that stress and SD could affect intestinal dysbiosis and increase colitogenic microbiota, which could contribute to the aggravating digestive disease. Melatonin concentrations in feces and colon tissue decreased under WS and SD. Melatonin treatment brought recovery of melatonin concentration in colon tissue and modulating dysbiosis of intestinal microbiota.

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“…Myricetin (purity >95%) was purchased from the National Institutes for Food and Drug Control (Beijing, China) and dissolved in water. For the induction of acute experimental colitis, the mice were exposed to 2% DSS for consecutive 7 days …”

Section: Methodsmentioning

confidence: 99%

“…For the induction of acute experimental colitis, the mice were exposed to 2% DSS for consecutive 7 days. [16][17][18][19][20]…”

Section: Pharmacological Compounds and Administrationmentioning

confidence: 99%

Potential of myricetin to restore the immune balance in dextran sulfate sodium-induced acute murine ulcerative colitis

Liu

Song

et al. 2020

Journal of Pharmacy and Pharmacology

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Objectives Myricetin is a bioactive compound in many edible plants with anti‐inflammatory and anticarcinogenic activity. The current study aimed to determine the protective effects and mechanism of myricetin against ulcerative colitis (UC). Methods Myricetin was orally administered at doses of 40 and 80 mg/kg to C57BL/6 mice with UC induced using dextran sulfate sodium. The disease‐associated index and colon length were determined at the end of the experiment, the proportion of Treg, Th1 and Th17 was analysed by cytometry, and cytokines were detected using ELISA. Key findings Myricetin (80 mg/kg) ameliorated the severity of inflammation in acute UC and significantly improved the condition. Myricetin (80 mg/kg) elevated the levels of IL‐10 and transforming growth factor β. In addition, the proportion of regulatory T cells significantly increased in mice in the myricetin treatment group. Conclusions Taking together, these results suggest that myricetin exhibits significant protective effects against UC and it could be used as a potential treatment for UC.

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Melatonin modulates adiponectin expression on murine colitis with sleep deprivation

Cited by 13 publications

References 37 publications

Melatonin controls microbiota in colitis by goblet cell differentiation and antimicrobial peptide production through Toll-like receptor 4 signalling

Melatonin controls microbiota in colitis by goblet cell differentiation and antimicrobial peptide production through Toll-like receptor 4 signalling

Melatonin in the colon modulates intestinal microbiota in response to stress and sleep deprivation

Potential of myricetin to restore the immune balance in dextran sulfate sodium-induced acute murine ulcerative colitis

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