Melittin suppresses EGF-induced cell motility and invasion by inhibiting PI3K/Akt/mTOR signaling pathway in breast cancer cells

Jeong, Yun-Jeong; Choi, Yong-Soo; Shin, Jeong Cheol; Cho, Hyun-Ji; Kang, Jeong Han; Park, Kwan‐Kyu; Choe, Jung‐Yoon; Bae, Young‐Seuk; Han, Sang‐Mi; Kim, Cheorl-Ho; Chang, Hyeun‐Wook; Chang, Young‐Chae

doi:10.1016/j.fct.2014.03.022

Cited by 112 publications

(97 citation statements)

References 35 publications

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“…25) AGA results in decreased proliferation of dermal papilloma cells and a reduction in hair follicle size. 26) Bee venom promotes proliferation of hDPCs and elongates hair follicles, thereby preventing AGA. Bee venom consists mainly of melittin (approximately 40-50%) as well as small quantities of polypeptides and low molecular compounds 23) ; the bee venom used in this study contained 56.7% melittin.…”

Section: Discussionmentioning

confidence: 99%

Bee Venom Promotes Hair Growth in Association with Inhibiting 5α-Reductase Expression

Park

Erdogan

Hwang

et al. 2016

Biological & Pharmaceutical Bulletin

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Alopecia is an important issue that can occur in people of all ages. Recent studies show that bee venom can be used to treat certain diseases including rheumatoid arthritis, neuralgia, and multiple sclerosis. In this study, we investigated the preventive effect of bee venom on alopecia, which was measured by applying bee venom (0.001, 0.005, 0.01%) or minoxidil (2%) as a positive control to the dorsal skin of female C57BL/6 mice for 19 d. Growth factors responsible for hair growth were analyzed by quantitative real-time PCR and Western blot analysis using mice skins and human dermal papilla cells (hDPCs). Bee venom promoted hair growth and inhibited transition from the anagen to catagen phase. In both anagen phase mice and dexamethasone-induced catagen phase mice, hair growth was increased dose dependently compared with controls. Bee venom inhibited the expression of SRD5A2, which encodes a type II 5α-reductase that plays a major role in the conversion of testosterone into dihydrotestosterone. Moreover, bee venom stimulated proliferation of hDPCs and several growth factors (insulin-like growth factor 1 receptor (IGF-1R), vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF)2 and 7) in bee venom-treated hDPCs dose dependently compared with the control group. In conclusion, bee venom is a potentially potent 5α-reductase inhibitor and hair growth promoter.

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Section: Discussionmentioning

confidence: 99%

Bee Venom Promotes Hair Growth in Association with Inhibiting 5α-Reductase Expression

Park

Erdogan

Hwang

et al. 2016

Biological & Pharmaceutical Bulletin

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“…Cell migration is a dynamic, wellorganized and complex process requiring co-ordination of many molecules in cells. Numerous studies have demonstrated that EGF activates its receptor, following resulting in increased cell migration in human tumorous cells (Cho et al, 2014;Jeong et al, 2014;Magi et al, 2014), thus EGF, via its receptor, plays an important role in cell migration and tumor invasion. Our research, using in vitro wound healing assay, clearly showed that EGF induced human cervical cancer SiHa cell migration (Figure 1).…”

Section: Discussionmentioning

confidence: 99%

Aquaporin 8 Involvement in Human Cervical Cancer SiHa Migration via the EGFR-Erk1/2 Pathway

Shi¹,

Tuokan²,

Chen³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Overexpression of aquaporins (AQPs) has been reported in several human cancers. Epidermal growth factor receptor (EGFR)-extracellular signal-regulated kinases 1/2 (Erk1/2) are associated with tumorigenesis and cancer progression and may upregulate AQP expression. In this study, we demonstrated that EGF (epidermal growth factor) induces SiHa cells migration and AQP8 expression. Wound healing results showed that cell migration was increased by 2.79-1.50-fold at 24h and 48h after EGF treatment. AQP8 expression was significantly increased (3.33-fold) at 48h after EGF treatment in SiHa cells. An EGFR kinase inhibitor, PD153035, blocked EGFinduced AQP8 expression and cell migration and AQP8 expression was decreased from 1.59-fold (EGF-treated) to 0.43-fold (PD153035-treated) in SiHa. Furthermore, the MEK (MAPK (mitogen-activated protein kinase)/ Erk (extracellular signal regulated kinase)/Erk inhibitor U0126 also inhibited EGF-induced AQP8 expression and cell migration. AQP8 expression was decreased from 1.21-fold (EGF-treated) to 0.43-fold (U0126-treated). Immunofluorescence microscopy further confirmed the results. Collectively, our findings show that EGF induces AQP8 expression and cell migration in human cervical cancer SiHa cells via the EGFR/Erk1/2 signal transduction pathway.

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“…Matrix metalloproteinase (MMP) 2 and MMP 9 are the downstream targets in Akt/mTOR signaling pathways. After activating Akt/ mTOR signaling pathways, MMP2 [5,6] and MMP9 [7,8] will express persistently, then they promote the metastasis of cervical cancer.…”

Section: Introductionmentioning

confidence: 99%

Effect of bortezomib on migration and invasion in cervical carcinoma HeLa cell

Shi¹,

Zhang

Yin

2015

Asian Pacific Journal of Tropical Medicine

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Melittin suppresses EGF-induced cell motility and invasion by inhibiting PI3K/Akt/mTOR signaling pathway in breast cancer cells

Cited by 112 publications

References 35 publications

Bee Venom Promotes Hair Growth in Association with Inhibiting 5α-Reductase Expression

Bee Venom Promotes Hair Growth in Association with Inhibiting 5α-Reductase Expression

Aquaporin 8 Involvement in Human Cervical Cancer SiHa Migration via the EGFR-Erk1/2 Pathway

Effect of bortezomib on migration and invasion in cervical carcinoma HeLa cell

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