Melting behaviour of a triple helical polysaccharide schizophyllan in aqueous solution

Yanaki, Toshio; Tabata, Kengo; Kojima, Takemasa

doi:10.1016/0144-8617(85)90035-9

Cited by 77 publications

(47 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Apparently, VST retains its triple helix structure, and thus its biological activity, at temperatures up to 135 "C (Yanaki et al 1983(Yanaki et al , 1985.…”

Section: Resultsmentioning

confidence: 99%

Trials with an orally and immersion-administered (ß-1,3 glucan as an immunoprophylactic against Aeromonas salmonicida in juvenile Chinook salmon Oncorhynchus tshawytscha

Nikl¹,

Evelyn²,

Albright³

1993

Dis. Aquat. Org.

View full text Add to dashboard Cite

Trials with an orally and immersion-administeredABSTRACT: A study was conducted to examine the effectiveness of a commercially available P-1,3glucan, VitaStim-Taito (VST), to stimulate immunity in juvenile chinook salmon Oncorhynchus tshawytscha against the bacterium Aeromonas salmonjcjda (As) causing furunculosis in salmon. VST had earlier been shown to be capable of elevating the potency of an anti-furunculosis vaccine when administered by injection. In the present study, 2 routes of administration were evaluated for their effectiveness: oral delivery and immersion. In the oral administration trials. VST in its crudest form was mixed (without the Asvaccine) into a steam-pelleted diet at a rate of 0, 0 01, 0.1, and 1.0 ' %. The 4 diets were fed to test fish at 2 %of body weight d.' After 7 d o n the diets, the f~s h were bath-challenged with As, the fish being maintained on the test diets for the rest of the exper~ment. VST added to the diet at either 0.1 or 1.0 ?&resulted in significant protection aga~nst the As challenge. In the immersion trials, anAs vaccine was administered to the test fish either alone or In combination with a pure or a crude form of VST The vaccine and the VST were also adm~nistered alone Following a bath challenge with virulent As at 21 d post-vaccination, no significant protection was noted in any of the groups tested, indicating that VST was inactive as an immunopotentiator by this route. The fish were, however, immunocompetent because protection occurred when they were injected with the vaccine alone.

show abstract

“…Apparently, VST retains its triple helix structure, and thus its biological activity, at temperatures up to 135 "C (Yanaki et al 1983(Yanaki et al , 1985.…”

Section: Resultsmentioning

confidence: 99%

Trials with an orally and immersion-administered (ß-1,3 glucan as an immunoprophylactic against Aeromonas salmonicida in juvenile Chinook salmon Oncorhynchus tshawytscha

Nikl¹,

Evelyn²,

Albright³

1993

Dis. Aquat. Org.

View full text Add to dashboard Cite

show abstract

“…Accordingly, it is safe to say that the critical concentration for the formation of DRSPG-1 weak gels lies in the range from 0.65 to 0.75 wt %. Yanaki et al 22 reported that SPG chains with M w ϭ 136,000 (value in DMSO), denatured at 150°C beforehand, tend to aggregate forming gels rather than to reform triple helices on the subsequent cooling if the concentration is above a critical value of 0.813 g/cm 3 . This critical concentration is slightly higher than that obtained in the present work for the NaOHinduced DRSPG-1 aqueous systems, which may be due to the difference in molecular weight between their sample and the present sample.…”

Section: Rheological Behavior Of Drspg Aqueous Systemmentioning

confidence: 98%

“…40 To clarify the origin of the strong shear-thinning behavior for DRSPG-1 aqueous systems, it is necessary to know what happens in the DR process. Sato et al 36 and Yanaki et al 22 reported that the renaturation of denatured SPG chains involves two different molecular processes consisting of the collapse of random coil chains to more compact conformation by intrachain hydrogen bonding and the formation of aggregates by interchain hydrogen bonding. Additionally, Stokke et al, 30,31 McIntire et al, 13,32 and Kitamura et al 33 found that the DR treatment results in various DRSPG structures including globular, linear, and circular forms as well as more complicated multichain clusters.…”

Section: Rheological Measurementsmentioning

confidence: 98%

“…16,19 -21 In addition, the conformational transition from triple helix to single coil chain can be also induced by heating the SPG aqueous solution above 135°C or by the addition of sodium hydroxide ([NaOH] Ͼ 0.2 mol/L) at room temperature. 22,23 Besides the conformational studies of SPG, a considerable amount of literature is devoted to investigating the gelation behaviors of SPG. Because of the spatial hindrance of larger side groups, it is difficult for SPG to form a gel by itself through aggregation of triple helices.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Rheological characterization of schizophyllan aqueous solutions after denaturation–renaturation treatment

2004

View full text Add to dashboard Cite

Schizophyllan (SPG) with a molecular weight of 2.6x10(6), designated SPG-1, is denatured and then renatured at a concentration of 1.8 wt % by alkalization-neutralization. The prepared denatured-renatured samples (DRSPG-1) are diluted to various concentrations and equilibrated for 10 days before rheological and intrinsic viscosity measurements. When concentration (C(p)) is above 0.75 wt %, DRSPG-1 aqueous systems have weak gel-type rheological properties. However, for 0.28 wt %

show abstract

“…Further, unlike Renibacterium salmoninarurn, grown with difficulty and therefore possibly expensive to mass produce, VST can be mass-produced at favourable cost. In addition, VST has already been chemically well defined, and, should it prove effective by feeding, its heat stability (135°C; Yanaki et al 1985) would permit it to withstand the high temperatures involved in incorporating it into steam-pelleted fish feeds.…”

Section: Discussionmentioning

confidence: 99%

Influence of seven immunostimulants on the immune response of coho salmon to Aeromonas salmonicida

Nikl¹,

Albright²,

Evelyn³

1992

Dis. Aquat. Org.

View full text Add to dashboard Cite

A study was conducted to evaluate the efficacy of 7 substances at potentiating a formalinlulled Aeromonas salmonicida bacterin in juvenile coho salmon Oncorhynchus kisutch. The substances were injected into the fish along with the bacterin. The fish were challenged 27 d later with viable A. salmonicida cells by 2 methods (cohabitation and immersion). The cumulative mortalities in each of the experimental groups was then determined. A significant and consistent increase in protection over the groups receiving only the A. sal~nonicida bacterin was observed with 3 of the substances tested. These were VitaStinl-Taito, lentinan and formalin-killed Renibacterium salmoninarum cells. One of these materials, VitaStim-Taito (a 8-1,3 glucan), showed particular promise for further studies.

show abstract

Melting behaviour of a triple helical polysaccharide schizophyllan in aqueous solution

Cited by 77 publications

References 12 publications

Trials with an orally and immersion-administered (ß-1,3 glucan as an immunoprophylactic against Aeromonas salmonicida in juvenile Chinook salmon Oncorhynchus tshawytscha

Trials with an orally and immersion-administered (ß-1,3 glucan as an immunoprophylactic against Aeromonas salmonicida in juvenile Chinook salmon Oncorhynchus tshawytscha

Rheological characterization of schizophyllan aqueous solutions after denaturation–renaturation treatment

Influence of seven immunostimulants on the immune response of coho salmon to Aeromonas salmonicida

Contact Info

Product

Resources

About