Members of the α‐amylase inhibitors family from wheat endosperm are major allergens associated with baker's asthma

Gómez, Luis; Martin, E.; Hernández, Dolores; Sánchez‐Monge, Rosa; Barber, Domingo; Pozo, Victoria del; Andrés, Belen de; Armentia, Alicia; Lahoz, Carlos; Salced, Gabriel; Palomino, Filar

doi:10.1016/0014-5793(90)80642-v

Cited by 119 publications

(73 citation statements)

References 27 publications

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“…Most of these inhibitors isolated from wheat, barley and rice are recognized by specific IgE when tested with sera from allergic patients (Barber et al, 1989;García-Casado et al, 1995;Gómez et al, 1990;Izumi et al, 1992;Sanchez-Monge et al, 1992;1996a,b). However, their IgE-binding capacities in vi tro are very different .…”

Section: Allergenic Propertíesmentioning

confidence: 99%

A Multigene Family of Trypsin/α-Amylase Inhibitors from Cereals

Carbonero¹,

Garcı́a-Olmedo²

1999

Seed Proteins

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Section: Allergenic Propertíesmentioning

confidence: 99%

A Multigene Family of Trypsin/α-Amylase Inhibitors from Cereals

Carbonero¹,

Garcı́a-Olmedo²

1999

Seed Proteins

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“…More than 10 homologous cDNA clones encoding the 14-16 kDa allergens have been obtained so far [8], indicating that the 14-16 kDa allergens are multigene products. DNA sequencing revealed that these cDNA clones all encoded proteins similar to wheat a-amylase inhibitor, barley trypsin inhibitor and the castor bean storage protein [9], which had been identified as major allergenic proteins associated with baker's asthma [10,11].…”

Section: Introductionmentioning

confidence: 99%

Reduction of 14–16 kDa allergenic proteins in transgenic rice plants by antisense gene

et al. 1996

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An antisense gene strategy was applied to suppress the 14-16 kDa allergen gene expression in maturing rice seeds. Gene constructs producing antisense RNAs of the 16 kDa allergen under the control of some rice seed-specific promoters were introduced into rice by electroporation. Immunoblot and RNA blot analyses of the seeds from the transgenic rice plants using the allergen-specific monoclonal antibody and a sequencespecific antisense RNA probe demonstrated that the 14-16 kDa allergen proteins and their transcripts of the seeds from several transgenic lines were present in much lower in amounts than those of the seeds from parental wild-type rice. The high levels of reduction observed were stably inherited in at least three generations.Key words: Rice allergen; Transgenic rice; Antisense RNA has been used experimentally to inhibit gene expression in bacteria, yeast, plant and animal cells [12], and the antisense strategy has also been reported to be practically applicable to transgenic crop plants, to which genes that produce antisense RNA are introduced to suppress the gene expression [13,14]. In the present study, the antisense genes for the 16 kDa allergen were introduced into rice by electroporation, and specific suppression of the 14-16 kDa allergen gene expression, resulting in the reduction of corresponding allergenic proteins in the mature seeds, was examined for regenerated transgenic rice plants and their progeny. The results show that antisense RNA markedly reduced the mRNA and protein contents of the 14-16 kDa allergens in the transgenic rice seeds. Materials and methods

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“…The disease is mainly caused by allergens present in wheat flour. Some of these allergens, such as fungal ␣-amylase, are derived from contaminants or additives (41), whereas others represent genuine wheat proteins or glycoproteins (18,22,23,42). At present, only few genuine wheat allergens have been identified, and it is not yet clear whether they are specific for baker's asthma or whether they are also recognized by patients suffering from wheat-associated food allergy or grass pollen allergy (26).…”

Section: Discussionmentioning

confidence: 99%

“…They include the members of the ␣ amylase inhibitor family (22), acyl-CoA oxidase, peroxidase (23), fructosebisphosphate aldolase, and recently thioredoxins (18). However, the great majority of the soluble wheat flour allergens have not yet been identified (24).…”

mentioning

confidence: 99%

Molecular and Immunological Characterization of a Wheat Serine Proteinase Inhibitor as a Novel Allergen in Baker's Asthma

Constantin

Quirce

Grote

et al. 2008

The Journal of Immunology

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IgE-mediated sensitization to wheat flour belongs to the most frequent causes of occupational asthma. A cDNA library from wheat seeds was constructed and screened with serum IgE from baker's asthma patients. One IgE-reactive phage clone contained a full-length cDNA coding for an allergen with a molecular mass of 9.9 kDa and an isoelectric point of 6. According to sequence analysis it represents a member of the potato inhibitor I family, a group of serine proteinase inhibitors, and thus is the first allergen belonging to the group 6 pathogenesis-related proteins. The recombinant wheat seed proteinase inhibitor was expressed in Escherichia coli and purified to homogeneity. According to circular dichroism analysis, it represented a soluble and folded protein with high thermal stability containing mainly β-sheets, random coils, and an α-helical element. The recombinant allergen showed allergenic activity in basophil histamine release assays and reacted specifically with IgE from 3 of 22 baker's asthma patients, but not with IgE from grass pollen allergic patients or patients suffering from food allergy to wheat. Allergen-specific Abs were raised to localize the allergen by immunogold electron microscopy in the starchy endosperm and the aleuron layer. The allergen is mainly expressed in mature wheat seeds and, despite an ∼50% sequence identity, showed no relevant cross-reactivity with allergens from other plant-derived food sources such as maize, rice, beans, or potatoes. Recombinant wheat serine proteinase inhibitor, when used in combination with other specific allergens, may be useful for the diagnosis and therapy of IgE-mediated baker's asthma.

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Members of the α‐amylase inhibitors family from wheat endosperm are major allergens associated with baker's asthma

Cited by 119 publications

References 27 publications

A Multigene Family of Trypsin/α-Amylase Inhibitors from Cereals

A Multigene Family of Trypsin/α-Amylase Inhibitors from Cereals

Reduction of 14–16 kDa allergenic proteins in transgenic rice plants by antisense gene

Molecular and Immunological Characterization of a Wheat Serine Proteinase Inhibitor as a Novel Allergen in Baker's Asthma

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