Membrane and extracellular antigens of Paracoccidioides brasiliensis (Mexo): Identification of a 28-kDa protein suitable for immunodiagnosis of paracoccidioidomycosis

Reis, Bernardo Sgarbi; Bozzi, Adriana; Prado, Fábio Luis Silva do; Pereira, Marta Máximo; Ferreira, Fátima; Godoy, Pérsio; Moro, Luciana; Pedroso, E.P.; Leite, M. Fátima; Góes, Alfredo M.

doi:10.1016/j.jim.2005.09.013

Cited by 20 publications

(23 citation statements)

References 25 publications

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“…Such serum data is in accordance with those reported by Reis et al (2005) because Pb28 has proven to be more sensitive and specific for PCM and Pbgp43 presented irregular results.…”

Section: Discussionsupporting

confidence: 92%

“…Serum tested by enzyme-linked immunosorbent assays (ELISA) for the extracellular antigens and membranes -MEXO -from P. brasiliensis presents a specificity of 96.6% compared to control groups and 81.2% in relation to groups with diverse infections (Reis et al 2005). A purified protein with a molecular weight of 28-kDa of MEXO, called Pb28, is the most specific antigen in the humoral immunological response of PCM.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Prostatic paracoccidioidomycosis: differential diagnosis of prostate cancer

Lopes¹,

Araújo

Santos

et al. 2009

Mem. Inst. Oswaldo Cruz

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Symptomatic prostatic paracoccidioidomycosis (PCM) is a very rare condition; however, it may express as a typical benign prostatic hyperplasia or a simulating prostatic adenocarcinoma. This case report presents PCM mimicking prostatic adenocarcinoma. The purpose of this paper is to call the general physician's attention to this important differential diagnosis.Key words: Paracoccidioides brasiliensis -paracoccidioidomycosis -prostatitis -prostate cancer Paracoccidioidomycosis (PCM) is an important deep mycosis in Latin America. It is caused by the dimorphic fungus Paracoccidioides brasiliensis, whose spores enter the body via the respiratory tract and evolve either asymptomatically or in a sub-acute or chronic manner. The parasite is able to provoke several clinical presentations due to its capacity to disseminate itself from the lungs through the lymphatic system or via the blood stream to any organ or system. PCM mainly affects adult males, preferentially in the lungs, mucosa, skin and phagocytic-monocytic system (Paniago et al. 2003. On a smaller scale, it reaches the nervous, musculoskeletal and suprarenal systems (Paniago et al. 2003). Its clinical incidence in the urinary tract, especially in prostatic injuries, is not well known. In a few necropsy studies involving disseminated PCM cases, its incidence varies from 2.7-9% (Salfelder et al. 1969, Begliomini et al. 1993.Prostatic diseases have become more common due to the increase in the population's age. Generally, they appear in males over 40-years-old, being more often bacterial and viral infections, benign hyperplasia and adenocarcinoma. Prostatic adenocarcinoma is the most fearful lesion due to its malignant and metastatic potential, and it is seen clinically in 10% of cases. Its diagnosis must always be set apart because its mortality is 3%, making it the greatest cause of morbi-mortality among men (Crawford 2003).Fungal prostatitis is unusual, having as its most common agents Coccidioides immitis, Candida albicans, Aspergillus sp., Cryptococcus neoformans and Blastomyces dermatitidis, which mainly affect patients who are immune-suppressed. The symptomatic attack of the prostate by PCM has rarely been described. Its potential severity justifies the reporting of this case. In this paper, infection of the prostate by P. brasiliensis is described, mimicking cancer with urethral obstruction and urgent expansion of the bladder. Case studyAAF, a 54-year-old married rural worker who was born in and is a current resident of São Sebastião do Maranhão, Minas Gerais, Brazil, presented in December 2003 with palate stomatitis associated with dysphagia and odynophagia. A biopsy of this injury revealed P. brasiliensis. He was treated with Sulfametoxazol (800 mg) and Trimethoprim (160 mg) daily in an irregular way for 20 months. In December 2004, he started to complain of dysuria, polyuria and urinary urgency and frequency, which culminated after one year in prompt urinary retention. Concomitantly, a stomatitis with moriforme injury of the palate appeared.A digital r...

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“…Such serum data is in accordance with those reported by Reis et al (2005) because Pb28 has proven to be more sensitive and specific for PCM and Pbgp43 presented irregular results.…”

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Prostatic paracoccidioidomycosis: differential diagnosis of prostate cancer

Lopes¹,

Araújo

Santos

et al. 2009

Mem. Inst. Oswaldo Cruz

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“…However, a few works in the literature make comparisons between patients presenting NPCM and patients without neurological involvement. Most of these studies make only diagnostic approaches, comparing the detection capacity of some antigen in the sera of patients with and without neuroparacoccidioidomycosis (NPCM) or evaluating the detection of these markers in the cerebrospinal fluid [7][8][9][10] . Therefore, there is a lack of studies that define singular clinical features that could distinguish the development of NPCM.…”

Section: Introductionmentioning

confidence: 99%

Paracoccidioidomycosis case series with and without central nervous system involvement

Pedroso

Lyon

Araújo

et al. 2012

Rev. Soc. Bras. Med. Trop.

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Introduction: Paracoccidioidomycosis (PCM) is the most important systemic mycosis in South America. Central nervous system involvement is potentially fatal and can occur in 12.5% of cases. This paper aims to contribute to the literature describing eight cases of neuroparacoccidioidomycosis (NPMC) and compare their characteristics with patients without neurological involvement, to identify unique characteristics of NPCM. Methods: A cohort of 213 PCM cases was evaluated at the Infectious Diseases Clinic of the University Hospital, Federal University of Minas Gerais, Brazil, from October 1976 to August 2008. Epidemiological, clinical, laboratory, therapeutic and follow-up data were registered. Results: Eight patients presented NPCM. The observed NPCM prevalence was 3.8%. One patient presented the subacute form of PCM and the other seven presented the chronic form of the disease. The parenchymatous form of NPCM occurred in all patients. 60% of the patients who proceeded from the north/ northeast region of Minas Gerais State developed NPCM. The neurological involvement of a mother and her son was observed. NPCM patients exhibited demographical and clinical profiles similar to what is described in the literature. When NPCM cases were compared to PCM patients, there were differences in relation to origin and positive PCM family history. Conclusions: The results corroborate the clinical view that the neurological findings are extremely important in the evaluation of PCM patients. Despite the limitations of this study, the differences in relation to patient's origins and family history point to the need of further studies to determine the susceptibility factors involved in the neurological compromise.

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“…The Mexo antigen has already been shown to have high sensitivity and specificity for the detection of antibodies against P. brasiliensis. Reis et al (2005) analysed the serological responses of 140 PCM patients to the Mexo antigen using immunoblotting, ELISA and IFI techniques. They observed a homogenous IgG response against the Mexo antigen with high sensitivity and specificity.…”

Section: Discussionmentioning

confidence: 99%

“…Antigens -The secreted and surface antigen Mexo was obtained from Pb18, a human source of a virulent strain of P. brasiliensis (Reis et al 2005). Yeast cells were cultured in YPD agar medium (0.5% yeast extract, 0.5% peptone, 1.5% D-glucose and 1.5% agar, pH 7.0) (Sigma, USA) at 35ºC and harvested on the seventh day of culture.…”

Section: Subjects Materials and Methodsmentioning

confidence: 99%