Membrane‐Bound Enzymes

Lenaz, Giorgio; Esposti, Mauro Degli

doi:10.1002/9783527616114.ch2

Cited by 2 publications

(1 citation statement)

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“…Note that this is different from the well known freeze-induced activation caused by an increased permeability of the membrane after freezing, when detergent is absent from the enzyme assay (Rehbein 1992;Fraser and McKenna 1998;Godiksen and Jessen 2001b). The importance of enzyme-lipid interactions has been demonstrated for the activity of cytochrome oxidase (Paradies and others 1994) and SR Ca 2+ -ATPase (Lee 1998) as well as for the activity of membrane-bound enzymes in general (Freedman 1981;Lenaz and Esposti 1994).…”

Section: Protein Ctf (G/g Muscle) (Mg/ml Ctf)mentioning

confidence: 86%

Sarcoplasmic Reticulum Ca²⁺‐ATPase and Cytochrome Oxidase as Indicators of Frozen Storage in Cod (Gadus morhua)

Godiksen

Hyldig

Jessen

2003

Journal of Food Science

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Activities of 2 membrane-bound enzymes, Ca 2+ -ATPase from the sarcoplasmic reticulum and cytochrome oxidase from the inner mitochondria membrane, were measured during frozen storage of cod. Enzyme activities were higher in cod muscle samples frozen at -30 °C than at -20 °C. Freezing-induced activation of both enzymes was observed and the activation was amplified by ice storage prior to freezing. Sensory evaluation conducted at 9 mo of frozen storage showed differences between the sensory properties of cod frozen immediately after catch and frozen after 3 d of storage on ice. These results indicated that the enzymes might be useful as indicators of quality changes by frozen storage.

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Section: Protein Ctf (G/g Muscle) (Mg/ml Ctf)mentioning

confidence: 86%

Sarcoplasmic Reticulum Ca²⁺‐ATPase and Cytochrome Oxidase as Indicators of Frozen Storage in Cod (Gadus morhua)

Godiksen

Hyldig

Jessen

2003

Journal of Food Science

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Mitochondria, oxidative stress, and antioxidant defences.

Lenaz¹,

Bovina²,

Formiggini³

et al. 1999

Acta Biochim Pol

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Mitochondria are strongly involved in production of reactive oxygen species, considered today as the main pathogenic agent of many diseases. A vicious circle of oxidative stress and damage to cellular structures can lead to either cell death by apoptosis or to a cellular energetic decline and ageing. The early involvement of mitochondria in apoptosis includes expression of pro-apoptotic factors, release of cytochrome c from the inter-membrane space and opening of the permeability transition pore: cytochrome c release appears to precede pore opening. The mitochondrial theory of ageing considers somatic mutations (deletions) of mitochondrial DNA induced by oxygen radicals as the primary cause of energy decline; experimentally, Complex I appears to be mostly affected. We have developed the Pasteur effect (enhancement of lactate production by mitochondrial inhibition) as a bio-marker of mitochondrial bioenergetics in human platelets, and found it to be decreased in aged individuals. Cells counteract oxidative stress by antioxidants; among lipophilic antioxidants coenzyme Q is the only one of endogenous biosynthesis; exogenous coenzyme Q, however, may protect cells from oxidative stress in vivo.

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Membrane‐Bound Enzymes

Cited by 2 publications

References 501 publications

Sarcoplasmic Reticulum Ca²⁺‐ATPase and Cytochrome Oxidase as Indicators of Frozen Storage in Cod (Gadus morhua)

Sarcoplasmic Reticulum Ca²⁺‐ATPase and Cytochrome Oxidase as Indicators of Frozen Storage in Cod (Gadus morhua)

Mitochondria, oxidative stress, and antioxidant defences.

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Membrane‐Bound Enzymes

Cited by 2 publications

References 501 publications

Sarcoplasmic Reticulum Ca2+‐ATPase and Cytochrome Oxidase as Indicators of Frozen Storage in Cod (Gadus morhua)

Sarcoplasmic Reticulum Ca2+‐ATPase and Cytochrome Oxidase as Indicators of Frozen Storage in Cod (Gadus morhua)

Mitochondria, oxidative stress, and antioxidant defences.

Contact Info

Product

Resources

About

Sarcoplasmic Reticulum Ca²⁺‐ATPase and Cytochrome Oxidase as Indicators of Frozen Storage in Cod (Gadus morhua)

Sarcoplasmic Reticulum Ca²⁺‐ATPase and Cytochrome Oxidase as Indicators of Frozen Storage in Cod (Gadus morhua)