Membrane-bound guaiacol peroxidases from maize (Zea mays L.) roots are regulated by methyl jasmonate, salicylic acid, and pathogen elicitors

Abstract

Plant peroxidases are involved in numerous cellular processes in plant development and stress responses. Four plasma membrane-bound peroxidases have been identified and characterized in maize (Zea mays L.) roots. In the present study, maize seedlings were treated with different stresses and signal compounds, and a functional analysis of these membrane-bound class III peroxidases (pmPOX1, pmPOX2a, pmPOX2b, and pmPOX3) was carried out. Total guaiacol peroxidase activities from soluble and microsomal fractions of maize roots were compared and showed weak changes. By contrast, total plasma membrane and washed plasma membrane peroxidase activities, representing peripheral and integral membrane proteins, revealed strong changes after all of the stresses applied. A proteomic approach using 2D-PAGE analysis showed that pmPOX3 was the most abundant class III peroxidase at plasma membranes of control plants, followed by pmPOX2a >pmPOX2b >pmPOX1. The molecular mass (63 kDa) and the isoelectric point (9.5) of the pmPOX2a monomer were identified for the first time. The protein levels of all four enzymes changed in response to multiple stresses. While pmPOX2b was the only membrane peroxidase down-regulated by wounding, all four enzymes were differentially but strongly stimulated by methyl jasmonate, salicylic acid, and elicitors (Fusarium graminearum and Fusarium culmorum extracts, and chitosan) indicating their function in pathogen defence. Oxidative stress applied as H2O2 treatment up-regulated pmPOX2b >pmPOX2a, while pmPOX3 was down-regulated. Treatment with the phosphatase inhibitor chantharidin resulted in distinct responses.

Reduction of Oxalate Levels in Tomato Fruit and Consequent Metabolic Remodeling Following Overexpression of a Fungal Oxalate Decarboxylase

“…Peroxiredoxin, an antioxidant that detoxifies H 2 O 2 (Mika et al, 2010) along with a major stress protein, Heat shock protein22 (Slt 183), might maintain the ionic homeostasis in E8.2-OXDC tomato. This might represent an adaptive mechanism in E8.2-OXDC tomato to protect the mitochondria from oxidative damage.…”

Selected reactive oxygen species and antioxidant enzymes in common bean after Pseudomonas syringae pv. phaseolicola and Botrytis cinerea infection

“…Due to a large number of their isoforms and heterogeneous regulation of their expression (Passardi et al 2005) they are good indicators of both biotic and abiotic stresses. Isoenzymes of class III peroxidases are mostly soluble apoplastic and cell wallbound forms (Mika et al 2010). In addition, they can be generated by post-transcriptional and post-translational modifications (Welinder et al 2002).…”

Assessment of Some Biomarkers under Submergence Stress in Some Rice Cultivars Varying in Responses

“…Moreover, the activity staining in gel for GPX (Figure 6) and GR also holds true for occurrence of polymorphisms and their variations within the tissue specific. Illustration with GPX isoforms with that of guaiacol as phenolic electron donor could also be able to generate free radicals [31]. A significant variation was recorded under anoxic and hypoxic condition in terms of ROS scavenging activities in plants.…”