Membrane-bound UDP-Glucose

Lezica, Rafael Pont; Romero, Pedro; Dankert, Marcelo A.

doi:10.1104/pp.58.5.675

Cited by 30 publications

(8 citation statements)

References 21 publications

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“…Under conditions where more than 80% of the allylic derivatives are degraded, we find that the P. vulgaris mannolipid is > 95% resistant to either phenol treatment or catalytic hydrogenation. Such a result has been reported for similar presumed lipid intermediates in other plant systems (3,14) and do suggest that the a-residue of these plant lipids is saturated.…”

supporting

confidence: 68%

“…A variety of reports (1,3,4,7,(13)(14)(15)(16) have demonstrated the synthesis of compounds resembling glycosyl-phosphoryl-polyprenols in higher plants, although evidence in these reports for a direct involvement of such compounds in glycoprotein synthesis was not presented. However, recent evidence from Elbein's laboratory (8,9) and our own (5) has provided strong support for the concept that such lipid intermediates do function in the glycosylation of plant proteins.…”

mentioning

confidence: 99%

“…Glycosyl-phosphoryl derivatives of allylic polyprenols have been shown to be degraded by treatment with phenol (50%, 68-70 C, 3 hr) or by catalytic hydrogenation, whereas similar derivatives of dolichol, which contain the saturated a-residue, are stable (10,11,14,20). Under conditions where more than 80% of the allylic derivatives are degraded, we find that the P. vulgaris mannolipid is > 95% resistant to either phenol treatment or catalytic hydrogenation.…”

mentioning

confidence: 99%

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Glycoprotein Synthesis in Plants

Delmer

Kulow²,

Ericson³

1978

Plant Physiol.

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MATERIALS AND METHODSIn Vitro Synthesis of Mannolipid. Preparation of extracts and production of mannolipid were scaled up from the general procedure of Ericson and Delmer (5). Developing seeds of P. vulgaris (14-17 mm in length) were harvested and the seed coats and embryos removed. The cotyledons (220 g fresh wt) were chopped with a razor blade at 4 C, and ground in a chilled mortar with 110 ml of 0.05 M tris-HCl (pH 8.5) containing 1 mM EDTA. The homogenate was centrifuged at 800g for 10 min and the supematant used for synthesis of mannolipid. Onequarter of the extract was used for preparation of high specific radioactivity mannolipid and the remainder for synthesis of larger quantities of low specific radioactivity mannolipid. Conditions for synthesis of the high specific radioactivity preparation were: crude extract, 28.5 ml; 0.2 M MnC12, 1.5 ml; and [GDP-'4C]mannose (New England Nuclear, specific radioactivity 210 mCi/mmol, 20 ,.Ci/ml), 0.075 ml. For the low specific radioactivity preparation, conditions were: crude extract, 120 ml; 0.2 M MnCl2, 6 ml; [GDP-14C]mannose (2.03 mCi/mmol, 9.09 tCi/ ml), 0.22 ml. The reactions were incubated for 15 min at 23 C and terminated, and the mannolipid extracted into chloroformmethanol as described previously (5)

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supporting

confidence: 68%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Glycoprotein Synthesis in Plants

Delmer

Kulow²,

Ericson³

1978

Plant Physiol.

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“…The identity of this compound was confirmed by treatment with strong acid, which produced free glucose (Fig. 5B) (8). The above described properties of the polar component are identical to those reported for dolichol-P-flglucose.…”

Section: Analysis Of the [14c]glucose Labeled Butanol Soluble Fractionsupporting

confidence: 51%

“…Ficaprenol was isolated from Ficus elastica leaves, then phosphorylated and its galactosylated derivative was obtained with an Acetobacter enzyme as reported by Romero et aL (7). Anhydroglucosan was prepared from salicine as described elsewhere (8). All other chemicals were commercial products.…”

Section: Introductionmentioning

confidence: 99%

Formation of lipid-linked sugars in mycelial and yeast-like forms ofMucor rouxii

et al. 1982

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Cell wall fragments from both yeast-like and mycelial forms of the dimorphic fungus Mucor rouxii were used as enzymatic preparations to study the synthesis and role of prenyl-phospho-sugars in these systems. In the presence of GDP [14C] mannose two main products were formed. One of them was characterized as dolichol-monophosphate beta-mannose on the following basis: solubility in organic solvents, behaviour upon paper chromatography, DEAE cellulose column chromatography, mild acid hydrolysis, alkali treatment, catalytic reduction and phenol degradation. The other product was identified as a glycoprotein containing a single mannose unit linked to a serine or threonine residue. It was degraded with pronase and by mild NaOH-NaBH4 treatment all the radioactivity was released as free mannitol. When UDP [14C] glucose was employed as sugar donor two butanol soluble components were isolated. One of them (25%) was characterized as dolichol-monophosphate-beta-glucose on the basis of the same criteria as described above. The other one (75%) was neutral and was not studied in detail. Mycelial enzymes were about 40 times more active in the synthesis of the dolichol derivatives. In addition, large amounts of glycogen were detected. The role that both dolichol derivatives might play in glycoprotein biosynthesis is discussed.

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Plant cell walls: morphology, biosynthesis and growth

Mackie¹

1985

Polysaccharides

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Membrane-bound UDP-Glucose

Cited by 30 publications

References 21 publications

Glycoprotein Synthesis in Plants

Glycoprotein Synthesis in Plants

Formation of lipid-linked sugars in mycelial and yeast-like forms ofMucor rouxii

Plant cell walls: morphology, biosynthesis and growth

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