Membrane Sampling with Microdialysis Coupled to HPLC/UV for On-line Simultaneous Determination of Melamine and Cyanuric Acid in Non-dairy Coffee Creamer

Chao, Yu-Ying; Wei, Yu-Tzu; Lee, Cheuch-Ting; Kou, Hwang‐Shang; Huang, Yeou‐Lih

doi:10.2116/analsci.27.1025

Cited by 3 publications

(3 citation statements)

References 26 publications

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“…Probe recovery is an important property of microdialysis systems [ 26 ]. Assuming that the perfusate can effectively dissolve the analyte, probe recovery mainly depends on the perfusate flow in the microdialysis probe [ 27 ]. When the perfusate flow is slow, analyte diffusion between the probe and the tissue fluid is closer to equilibrium, leading to a higher recovery rate.…”

Section: Resultsmentioning

confidence: 99%

A novel microemulsion-based isotonic perfusate modulated by Ringer’s solution for improved microdialysis recovery of liposoluble substances

Zhang¹,

Wang²,

Shen³

et al. 2018

J Nanobiotechnol

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BackgroundMicrodialysis is promising technique for dynamic microbiochemical sampling from tissues. However, the application of typical aqueous perfusates to liposoluble substances is limited. In this study, a novel microemulsion (ME)-based isotonic perfusate (RS-ME) was prepared to improve the recovery of liposoluble components using microdialysis probes.ResultsBased on pseudo-ternary phase diagrams and comparisons of the ME area, Kolliphor® EL and Transcutol® P were selected as the surfactant and co-surfactant, respectively, with a weight ratio (Km) of 2:1 and ethyl oleate as the oil phase. The ME was mixed with Ringer’s solution at a 1:6 ratio (v/v) to obtain the isotonic RS-ME. The droplet size distribution of the ME in RS-ME was 78.3 ± 9.2 nm, with a zeta potential of − 3.5 ± 0.3 mV. By microdialysis perfusion, RS-ME achieved higher recovery rates of the poorly water-soluble compounds evodiamine (EVO) and ruthenium (RUT), i.e., 58.36 ± 0.57% and 49.40 ± 0.57%, respectively, than those of 20% (v/v) PEG 400 Ringer's solution (RS-PEG) and 10% (v/v) ethanol Ringer’s solution (RS-EtOH). In vivo microdialysis experiments confirmed that RS-ME captured EVO and RUT molecules around the dialysis membrane more efficiently and exhibited less spreading than RS-PEG and RS-EtOH.ConclusionsOwing to the nanosized droplets formed by lipid components in the RS-ME and the limited dispersion out of the dialysis membrane, we obtained good biocompatibility and reliable dialysis results, without affecting the tissue microenvironment. As a novel perfusate, RS-ME provides an easy and reliable approach to the microdialysis sampling of fat-soluble components.

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Section: Resultsmentioning

confidence: 99%

A novel microemulsion-based isotonic perfusate modulated by Ringer’s solution for improved microdialysis recovery of liposoluble substances

Zhang¹,

Wang²,

Shen³

et al. 2018

J Nanobiotechnol

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“… 2–100 mg/L (CYA) 0.02–5 mg/L (MEL) 0.03 mg/L (CYA) 0.001 mg/L (MEL) Chao et al (2011a) An on-line membrane sampling with microdialysis/HPLC with UV detection method was developed for the simultaneous determination of CYA and MEL in non-dairy coffee creamer. 0.5–100 mg/L (CYA) 0.01–5 mg/L (MEL) 0.15 mg/L (CYA) 0.003 mg/L (MEL) Chao et al (2011b) LC–MS/MS CYA is isolated with a graphitic carbon black solid-phase extraction column, residues are separated from matrix using a porous graphitic carbon column, and then analyzed with electrospray ionization using a triple quadrupole mass spectrometer. 10–1000 μg/kg 3.5 μg/kg Karbiwnyk et al (2009) LC-MS The method used diatomaceous earth extraction columns for sample preparation and negative ion electrospray with a cyano column for CYA quantification.…”

Section: Introductionmentioning

confidence: 99%

Green detection of trace cyanuric acid and free chlorine together via ion chromatography

et al. 2022

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Chlorinated cyanurates (CCAs) are a type of disinfectants currently used worldwide for fight of Coronavirus. However, CCAs upon dosed into water can release not only free chlorine (FC), a strong disinfectant, but also cyanurate (CYA), a persistent compound potentially harmful to human and environment. Therefore, detecting CYA and FC in water are very important not only for ensuring sufficient disinfection but also for monitoring the impacts of FC and CYA on receiving watershed. However, conventional analytical methods for them are mostly based on colorimetric methods, which have high method detection limits (MDLs) and rely on chemical reactions that are likely sensitive to coexisting chemicals. To overcome these issues, we herein proposed a facile and reaction-free method to detect CYA and FC together in just one run by ion chromatography (IC) equipped with both conductivity and ultraviolet absorbance detectors. The method features obvious advantages over colorimetric methods in being lower MDLs (3.6 μg/L for CYA and 9.0 μg/L for FC), environmental-friendly (i.e., no organic solvent involved), and more resistant to alkaline solution. With this method, trace levels of CYA (i.e., 34–44 μg/L), which were nondetectable by conventional method, were found in two river water samples, implying that the local environment was already polluted by CCAs during the pandemic period. Overall, this study demonstrates a robust tool that may assist better understanding and monitoring the fate and transport of trace CCA derivatives in water.

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“…In the scientific literature many methods have been proposed for the analysis of melamine in food and pet foodstuffs 2 Journal of Chemistry [19]. They are mainly based on common methodologies as gas chromatography (GC) and high-performance liquid chromatography (HPLC) in combination with different selective detection techniques [19][20][21][22][23][24][25][26][27]. More recent methods using electrochemical [28], surface-enhanced Raman spectroscopy (SERS) [29], infrared spectroscopy [30], colorimetric sensor based on polydiacetylene (PDA) liposomes [31], and quantum dots-based fluorescence method [32] have been published to detect melamine.…”

Section: Introductionmentioning

confidence: 99%

A Simple HPLC‐DAD Method for the Analysis of Melamine in Protein Supplements: Validation Using the Accuracy Profiles

Montesano

Gennari

Festa

et al. 2013

Journal of Chemistry

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The study presents a fully validated simple high-performance liquid chromatography method with diode array detection (HPLC-DAD), able to accurately determine the melamine, fraudulently added, in protein supplements, commonly used from healthy adults to enhance exercise or sport performance. The validation strategy was intentionally oriented towards routine use and the reliability of the method rather than extreme performance. For this reason, validation by accuracy profile, including estimation of uncertainty, was chosen. This procedure, based on the concept of total error (bias + standard deviation), clearly showed that this method was able to determine melamine over the range of 0.05-3.0 mg Kg −1 , selected by taking into account the maximum residue levels (MRLs) proposed by European legislation to distinguish between the unavoidable background presence of melamine and unacceptable adulteration. The accuracy profile procedure established that at least 95% of the future results obtained with the proposed method would be within the ±15% acceptance limits of the validated HPLC-DAD method over the whole defined concentration range.

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Membrane Sampling with Microdialysis Coupled to HPLC/UV for On-line Simultaneous Determination of Melamine and Cyanuric Acid in Non-dairy Coffee Creamer

Cited by 3 publications

References 26 publications

A novel microemulsion-based isotonic perfusate modulated by Ringer’s solution for improved microdialysis recovery of liposoluble substances

A novel microemulsion-based isotonic perfusate modulated by Ringer’s solution for improved microdialysis recovery of liposoluble substances

Green detection of trace cyanuric acid and free chlorine together via ion chromatography

A Simple HPLC‐DAD Method for the Analysis of Melamine in Protein Supplements: Validation Using the Accuracy Profiles

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