Membrane transport enhancement of chlorin e6‐polyvinylpyrrolidone and its photodynamic efficacy on the chick chorioallantoic model

Chin, William W.; Heng, Paul Wan Sia; Lim, Pei Li; Lau, Weber Kam On; Olivo, Malini

doi:10.1002/jbio.200810005

Cited by 10 publications

(4 citation statements)

References 25 publications

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“…For example, the topical application of chlorines showed a larger occlusion at 10 J/cm 2 doses with irradiance of 10 mW/cm 2 when compared to 15 and 20 J/cm 2 (with 15 and 20 mW/cm 2 , respectively). This difference for high doses may be related to the depletion of oxygen or faster photobleaching [43].…”

Section: Discussionmentioning

confidence: 99%

Vascular Effects of Photodynamic Therapy with Curcumin in a Chorioallantoic Membrane Model

Buzzá

Freitas

Moriyama

et al. 2019

IJMS

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Photodynamic Therapy (PDT) is a treatment that requires light, a photosensitizing agent, and molecular oxygen. The photosensitizer is activated by light and it interacts with the oxygen that is present in the cellular microenvironment. The molecular oxygen is transformed into singlet oxygen, which is highly reactive and responsible for the cell death. Therefore, PS is an important element for the therapy happens, including its concentration. Curcumin is a natural photosensitizer and it has demonstrated its anti-inflammatory and anti-oxidant effects that inhibit several signal transduction pathways. PDT vascular effects of curcumin at concentrations varying from 0.1 to 10 mM/cm2 and topical administration were investigated in a chick Chorioallantoic Membrane (CAM) model. The irradiation was performed at 450 nm, irradiance of 50 mW/cm2 during 10 min, delivering a total fluence of 30 J/cm2. The vascular effect was followed after the application of curcumin, with images being obtained each 30 min in the first 3 h, 12 h, and 24 h. Those images were qualitatively and quantitatively analyzed with a MatLAB®. Curcumin was expected to exhibit a vascular effect due to its angio-inhibitory effect. Using curcumin as photosensitizer, PDT induced a higher and faster vascular effect when compared to the use of this compound alone.

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Section: Discussionmentioning

confidence: 99%

Vascular Effects of Photodynamic Therapy with Curcumin in a Chorioallantoic Membrane Model

Buzzá

Freitas

Moriyama

et al. 2019

IJMS

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“…The sensitivity of Ce6-PVP was more than 80%, however, the specificity remained low. We have recently reported that the new formulation of Ce6-PVP (> 95% purity level of active Ce6) demonstrated a higher sensitivity (98%) and specificity (82%) on the CAM model [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

“…By employing a chick chorioallantoic membrane model, Ce6-PVP was shown to selective accumulate in bladder tumors xenografts and had a faster clearance from normal CAM when administered topically compared to systematic administration [ 14 ]. The uptake ratio of Ce6-PVP was found to have a 2-fold increase across the CAM when compared to that of Ce6, indicating that PVP was able to facilitate diffusion of Ce6 across the membrane [ 15 ]. Furthermore, Ce6-PVP had less in vivo systemic phototoxic effect compared to Ce6 alone after light irradiation in photodynamic therapy in mice bearing tumors [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

In-vivo optical detection of cancer using chlorin e6 – polyvinylpyrrolidone induced fluorescence imaging and spectroscopy

et al. 2009

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BackgroundPhotosensitizer based fluorescence imaging and spectroscopy is fast becoming a promising approach for cancer detection. The purpose of this study was to examine the use of the photosensitizer chlorin e6 (Ce6) formulated in polyvinylpyrrolidone (PVP) as a potential exogenous fluorophore for fluorescence imaging and spectroscopic detection of human cancer tissue xenografted in preclinical models as well as in a patient.MethodsFluorescence imaging was performed on MGH human bladder tumor xenografted on both the chick chorioallantoic membrane (CAM) and the murine model using a fluorescence endoscopy imaging system. In addition, fiber optic based fluorescence spectroscopy was performed on tumors and various normal organs in the same mice to validate the macroscopic images. In one patient, fluorescence imaging was performed on angiosarcoma lesions and normal skin in conjunction with fluorescence spectroscopy to validate Ce6-PVP induced fluorescence visual assessment of the lesions.ResultsMargins of tumor xenografts in the CAM model were clearly outlined under fluorescence imaging. Ce6-PVP-induced fluorescence imaging yielded a specificity of 83% on the CAM model. In mice, fluorescence intensity of Ce6-PVP was higher in bladder tumor compared to adjacent muscle and normal bladder. Clinical results confirmed that fluorescence imaging clearly captured the fluorescence of Ce6-PVP in angiosarcoma lesions and good correlation was found between fluorescence imaging and spectral measurement in the patient.ConclusionCombination of Ce6-PVP induced fluorescence imaging and spectroscopy could allow for optical detection and discrimination between cancer and the surrounding normal tissues. Ce6-PVP seems to be a promising fluorophore for fluorescence diagnosis of cancer.

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“…The CAM continues to be a common in vivo model used to study tumor angiogenesis, anti-angiogenic drugs, [89][90][91][92][93][94] tumor vascular targeting, 95 metastasis, 96 97 ion transport, 98 allergens, 99 transplantation, 100 contraceptives, 101 102 effects of hyperglycemia, 103 and photodynamic therapy. 104 Since the CAM capillary network is so dense, it is comparable to the capillary plexus of the eye; 105 therefore, it has been extensively used as an ocular model 106 for photodynamic therapy (PDT) with photosensitizing drugs. 107 109 In addition, the CAM was initially used as an alternative to the Draize test, which originally involved exposing the eyes of rabbits to chemicals to determine irritation.…”

Section: Chorioallantoic Membrane (Cam)mentioning

confidence: 99%

Testing Nanoparticles for Angiogenesis-Related Disease: Charting the Fastest Route to the Clinic

Sarsons¹,

Tekrony²,

Yaehne³

et al. 2014

j biomed nanotechnol

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Recently, nanoparticles (NPs) have been established as ideal drug delivery vehicles for treating cancer. This is due to the enhanced permeability and retention (EPR) effect that is a direct result of the angiogenic nature of the tumor tissue and its ability to sequester chemotherapeutics from healthy tissues. Ideal drug delivery nanocarriers will exploit the EPR effect, accumulate in the tumorous tissue, and be able to release the drugs at a high concentration where needed, thereby reducing undesirable side effects. In order to determine ideal NP qualities that enable drugs to be delivered in such a manner, extensive testing in biological systems is required. However, it is impractical to study new potential nanocarriers in humans or in mammalian models due to the potential adverse consequences, low throughput, and high cost. Simpler models would allow for higher throughput screening of nanocarrier vehicles. This review outlines the most recent advances in alternative model assays and their significance in testing NPs en route to the clinic. In decreasing complexity, we examine zebrafish embryos, the chorioallantoic membrane of the chicken embryo, multicell static and flow-based assays, and single cell assays for efficacy, accuracy and utility as predictors for human therapeutic outcomes.

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Membrane transport enhancement of chlorin e6‐polyvinylpyrrolidone and its photodynamic efficacy on the chick chorioallantoic model

Cited by 10 publications

References 25 publications

Vascular Effects of Photodynamic Therapy with Curcumin in a Chorioallantoic Membrane Model

Vascular Effects of Photodynamic Therapy with Curcumin in a Chorioallantoic Membrane Model

In-vivo optical detection of cancer using chlorin e6 – polyvinylpyrrolidone induced fluorescence imaging and spectroscopy

Testing Nanoparticles for Angiogenesis-Related Disease: Charting the Fastest Route to the Clinic

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