Membrane type matrix metalloproteinases (MMPs) show differential expression in non-small cell lung cancer (NSCLC) compared to normal lung: Correlation of MMP-14 mRNA expression and proteolytic activity

Atkinson, Jennifer M.; Pennington, C.; Martin, Sandie W.; Anikin, V.; Mearns, Alan J.; Loadman, Paul M.; Edwards, Dylan R.; Gill, Jason H.

doi:10.1016/j.ejca.2007.05.009

Cited by 47 publications

(43 citation statements)

References 28 publications

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“…However, MT1-MMP does not require additional activation, due to its capacity to be presented on the cell membrane in its active form [34]. MT1-MMP is an essential pericellular collagenolytic enzyme during tumor growth, angiogenesis, and invasion [35]. The overexpression of MT1-MMP was observed in human lung cancer, colon cancer, breast cancer, prostate cancer, and glioblastomas compared with benign tumor or normal tissue [36][37][38][39].…”

Section: Discussionmentioning

confidence: 99%

MT1-MMP is not a good prognosticator of cancer survival: evidence from 11 studies

Lin

et al. 2014

Tumor Biol.

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MT1-MMP exhibits diverse expressions in patients with cancer and could be considered as potential prognostic biomarker of cancer. We performed a meta-analysis aiming to provide more sufficient evidence that MT1-MMP expression is associated with poor overall survival in several types of cancers. We systematically searched the studies from databases and carefully identified based on eligibility criteria. The association between MT1-MMP expression and overall survival in cancers was estimated using Review Manager. A total of 11 literatures which included 1,918 cancer patients were combined in the final analysis. Meta-analysis revealed that MT1-MMP overexpression was associated with an unfavorable overall survival and the pooled hazard ratio (HR) and corresponding 95 % confidence interval (CI) was 2.46 (95 % CI 1.75-3.47). From subgroup analyses, we identified that MT1-MMP was an independent prognostic factor for lung cancer and gastric cancer, and HRs (95 % CI) were 3.73 (95 % CI 2.67-5.21) and 2.46 (95 % CI 1.69-3.59), respectively. In conclusion, MT1-MMP is a potential prognostic factor in human cancers.

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Section: Discussionmentioning

confidence: 99%

MT1-MMP is not a good prognosticator of cancer survival: evidence from 11 studies

Lin

et al. 2014

Tumor Biol.

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“…MMPs are an important part of these proteolytic enzymes [39]. They are highly expressed in multiple malignancies, including breast cancer [40], ovarian cancer [41], lung cancer [42] and colon cancer [43]. In addition, it has been reported that blockade of the expression and activity of MMPs could be the major cause of reduced motility and metastasis of cancer cells [44].…”

Section: Discussionmentioning

confidence: 99%

Dickkopf-Related Protein 2 is Epigenetically Inactivated and Suppresses Colorectal Cancer Growth and Tumor Metastasis by Antagonizing Wnt/β-Catenin Signaling

Wang

Yue

Shao

et al. 2017

Cell Physiol Biochem

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Background/Aims: Aberrant activation of the Wnt/β-catenin signaling pathway plays a key role in the pathogenesis of multiple tumors including digestive cancers. Recent studies have reported that Dickkopf-related protein 2 (DKK2) is epigenetically inactivated in numerous types of cancers and that its gene products exhibit tumor-suppressive properties. However, the biological functions and underlying molecular mechanisms of DKK2 in colon carcinoma remains obscure. Methods: We examined the expression of DKK2 in colon tumor cell lines by RT-PCR and its promoter methylation status in colon tumor cell lines and primary tumors by methylation-specific PCR (MSP). Ectopic expression of DKK2 was measured by RT-PCR prior to the other experiments. To investigate the function of DKK2, we assayed colony formation and cell proliferation, utilized flow cytometric analyses of the cell cycle and acridine orange/ethidium bromide (AO/EB) fluorescence staining for apoptosis, and examined wound healing, transwell migration and tumor growth in vivo. Western blots were used to explore the mechanisms of DKK2 in epithelial- mesenchymal transition and canonical Wnt/β-catenin signaling. Results: We show here that downregulation or silencing of DKK2 was closely associated with the hypermethylation status of its promoter and that DKK2 expression could be restored by demethylation treatment. Methylation of the DKK2 promoter was detected in nearly all tumors and tumor-adjacent tissues, but not in normal colon tissues. Ectopic expression of DKK2 in colon cell lines HCT116 and HT-29 inhibited colony formation and cell viability by inducing cell cycle G0/G1 arrest and apoptosis, and growth of stable DKK2-infected HCT116 cells in nude mice was decreased compared to controls. Furthermore, DKK2 restrained cell migration through partial reversal of epithelial-to- mesenchymal transition and also by downregulating several stem cell markers. Our data further showed that restoration of DKK2 expression resulted in downregulation of active β-catenin and its downstream target genes. Conclusion: DKK2 appears to be a functional tumor suppressor regulating tumorigenesis of colorectal cancer by antagonizing Wnt/β-catenin signaling.

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“…MMPs such as interstitial collagenase (MMP-1) and type IV collagenases (MMP-2, MMP-9) are involved in the initial breakdown of collagen and basement membrane components during tumor growth and invasion. Overexpression of MMP-1, MMP-2 and MMP-9, MMP-11, MMP-13, MMP-14-17, MMP-24 and MMP-25, and TIMP-1 and TIMP-2 positively correlated with more advanced-stage disease and highly invasive and meta-static potential of carcinomas [7,8,9]. …”

Section: Discussionmentioning

confidence: 99%

Matrix Metalloproteinase Activity in Early-Stage Lung Cancer

et al. 2013

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Background: The matrix metalloproteinases (MMPs)-2, -9 and -7 are thought to be associated with tumor invasion, metastasis, and angiogenesis. However, their possible roles in early-stage lung cancer are not clear. We measured the activity of MMP-2, -7 and -9 in early-stage lung cancer tissues. Material and Methods: Normal lung tissues and cancer tissues were collected from 60 consecutive stage-I non-small cell lung cancer (NSCLC) patients. The activities of MMP-2 and MMP-9 were determined by gelatin zymography, and the activity of MMP-7 was determined by casein zymography. Furthermore, the ratio of the active form of MMP-2 in tumor tissue (T) compared with normal tissue (N) was determined, and the survival in the groups with different MMP-2 T:N ratio was compared. Results: The activity of both MMP-2 and MMP-9 was detected in all cancer and normal tissues. Interestingly, MMP-9 activity was significantly reduced, whereas MMP-2 activity was significantly increased, in cancer tissues compared to normal tissues. The survival rate of the MMP-2 T:N ratio > 2.5 group was 57.45%, which was significantly reduced compared with that of the T:N ratio ≤ 2.5 group (86.78%). Conclusion: Our findings suggest that MMP-2, but not MMP-9 and MMP-7, may be implicated in early-stage tumor invasion, metastasis, and angiogenesis in NSCLC.

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Membrane type matrix metalloproteinases (MMPs) show differential expression in non-small cell lung cancer (NSCLC) compared to normal lung: Correlation of MMP-14 mRNA expression and proteolytic activity

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Cited by 47 publications

References 28 publications

MT1-MMP is not a good prognosticator of cancer survival: evidence from 11 studies

MT1-MMP is not a good prognosticator of cancer survival: evidence from 11 studies

Dickkopf-Related Protein 2 is Epigenetically Inactivated and Suppresses Colorectal Cancer Growth and Tumor Metastasis by Antagonizing Wnt/β-Catenin Signaling

Matrix Metalloproteinase Activity in Early-Stage Lung Cancer

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