Memory of 5-min heat stress in <i>Arabidopsis thaliana</i>

Oyoshi, Kohey; Katano, Kazuma; Yunose, Mai; Suzuki, Nobuhiro

doi:10.1080/15592324.2020.1778919

Cited by 15 publications

(11 citation statements)

References 30 publications

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“…Estimates of how global warming will affect ecosystems is a central task of current (plant) biological research. Heat affects plant metabolism and metabolic regulation by its impact on enzyme activities, membrane composition and rigidity, and signaling cascades [ 7 , 8 ]. Heat stress typically results in increased generation of reactive oxygen species (ROS), which can cause membrane damage, induce cytotoxic free-radical reactions, and impact on redox regulation and cellular signaling [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Indications for a Central Role of Hexokinase Activity in Natural Variation of Heat Acclimation in Arabidopsis thaliana

Atanasov¹,

Fürtauer²,

Nägele³

2020

Plants

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Diurnal and seasonal changes of abiotic environmental factors shape plant performance and distribution. Changes of growth temperature and light intensity may vary significantly on a diurnal, but also on a weekly or seasonal scale. Hence, acclimation to a changing temperature and light regime is essential for plant survival and propagation. In the present study, we analyzed photosynthetic CO2 assimilation and metabolic regulation of the central carbohydrate metabolism in two natural accessions of Arabidopsis thaliana that originate from north western Russia and south Italy during exposure to heat and a combination of heat and high light. Our findings indicate that it is hardly possible to predict photosynthetic capacities under combined stress from single stress experiments. Further, capacities of hexose phosphorylation were found to be significantly lower in the Italian than in the Russian accession, which could explain an inverted sucrose-to-hexose ratio. Together with the finding of significantly stronger accumulation of anthocyanins under heat/high light, these observations indicate a central role of hexokinase activity in the stabilization of photosynthesis and carbohydrate metabolism during environmental changes.

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Section: Introductionmentioning

confidence: 99%

Indications for a Central Role of Hexokinase Activity in Natural Variation of Heat Acclimation in Arabidopsis thaliana

Atanasov¹,

Fürtauer²,

Nägele³

2020

Plants

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“…While temperature acclimation of plants typically can be observed after days of exposure to non-lethal cold or heat (Atanasov et al , 2020; Garcia-Molina et al , 2020), transient temperature changes and plant stress response occurs within minutes or hours. Interestingly, Arabidopsis thaliana was found to memorize already 5 minutes of heat stress which indicates a tightly regulated molecular network involved in heat stress response (Oyoshi et al , 2020). High temperature, e.g., between 35°C – 40°C, is well known to result in a reduced rate of photosynthesis (Sharkey, 2005) which has also been observed in the present study.…”

Section: Discussionmentioning

confidence: 99%

Integration of plant carbohydrate dynamics by Fourier polynomials

Seydel

Biener

Brodsky

et al. 2021

Preprint

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Quantification of system dynamics is a central aim of mathematical modelling in biology. Defining experimentally supported functional relationships between molecular entities by mathematical terms enables the application of computational routines to simulate and analyse the underlying molecular system. In many fields of natural sciences and engineering, trigonometric functions are applied to describe oscillatory processes. As biochemical oscillations occur in many aspects of biochemistry and biophysics, Fourier analysis of metabolic functions promises to quantify, describe and analyse metabolism and its reaction towards environmental fluctuations. Here, Fourier polynomials were developed from experimental time-series data and combined with block diagram simulation of plant metabolism to study heat shock response of photosynthetic CO2 assimilation and carbohydrate metabolism. Findings suggest that increased capacities of starch biosynthesis stabilize photosynthetic CO2 assimilation under transient heat exposure. Among soluble sugars, fructose concentrations were observed to fluctuate least under heat exposure which might be the consequence of high respiration rates under elevated temperature. Finally, Col-0 and two mutants of Arabidopsis thaliana with deficiencies in starch and sucrose metabolism were discriminated by fundamental frequencies of Fourier polynomials across different experiments. This suggests balance modelling based on Fourier polynomials as a suitable approach for mathematical analysis of dynamic plant-environment interactions.

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“…Within minutes of exposure to heat stress (HS), a heat stress response (HSR) is initiated in plants as a countermeasure (Oyoshi et al 2020; Sarkar et al 2014). HSR involves a multitude of adaptive molecular processes, including activation of signalling components and transcription factors (Singh et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Hsp70, sHsps and ubiquitin proteins modulate HsfA6a‐mediated Hsp101 transcript expression in rice (Oryza sativa L.)

Singh

Sarkar

Grover

2021

Physiologia Plantarum

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Hsp100 chaperones disaggregate the aggregated proteins and are vital for maintenance of protein homeostasis. The level of Hsp100 synthesised in the cells has a bearing on the survival of plants under heat stress. The Hsp100 transcription machinery is activated within minutes of the onset of heat stress. The heat shock factor HsfA6a plays a major role in the transcriptional regulation of the Hsp101 gene in rice plants. Through yeast‐2‐hybrid library screening, we identified small heat shock proteins (sHSPs), Hsp70 and ubiquitin as HsfA6a interacting proteins (HIPs). The bimolecular fluorescence complementation assays showed the physical interaction of HsfA6a with Hsp16.9A‐CI and Hsp18.0‐CII in the cytosolic region and with cHsp70‐1 in the nucleus. With the Hsp101 promoter: reporter gene assays, using yeast cells and rice protoplasts, we show that CI‐sHsps and CII‐sHsps are negative regulators and Hsp70 positive regulator of the HsfA6a activity in modulation of Hsp101 transcription. We also noted that the HsfA6a interactors, Hsp70 and CI‐sHsps and CII‐sHsps, physically interact with each other. We noted that HsfA6a binds with the CI‐sHsp and Hsp70 promoters, implying that HsfA6a has a role in transcriptional regulation of its interacting proteins. Furthermore, we noted that the mutation of the ubiquitin/sumoylation acceptor site lysine 10 to alanine (K10A) of HsfA6a enhanced its DNA binding potential on the Hsp101 promoter, implying that these modifiers are possibly involved in modulation of HsfA6a activity. Our work shows that Hsp70, CI‐sHsps and CII‐sHsp, and ubiquitin proteins coordinate with HsfA6a in mediating the Hsp101 transcription process in rice.

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Memory of 5-min heat stress in Arabidopsis thaliana

Cited by 15 publications

References 30 publications

Indications for a Central Role of Hexokinase Activity in Natural Variation of Heat Acclimation in Arabidopsis thaliana

Indications for a Central Role of Hexokinase Activity in Natural Variation of Heat Acclimation in Arabidopsis thaliana

Integration of plant carbohydrate dynamics by Fourier polynomials

Hsp70, sHsps and ubiquitin proteins modulate HsfA6a‐mediated Hsp101 transcript expression in rice (Oryza sativa L.)

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