Mempelajari Viabilitas Enkapsulasi Sel Probiotik (Lactobacillus Plantarum Dan Streptococcus Thermophilus) Pada Es Krim

Bilang, Mariyati; Tahir, Mulyati M.; Haedar, Darmayanti

doi:10.20956/canrea.v1i1.21

Cited by 4 publications

(7 citation statements)

References 7 publications

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“…More water is absorbed when humidity rises, lowering tensile flexibility and strength. The current findings corroborated a prior observation that the encapsulant matrix's durability decreased as the storage period increased [48]. At both room and cold temperatures, the CMFH from OPT maintained probiotic viability for 28 d. The study discovered that probiotics encapsulated in OPT and OPF cellulose nanofiber hydrogel (CNFH) were alive for 5 and 21 days, respectively [33].…”

Section: -2-viability Of Strain B1295supporting

confidence: 89%

Cellulose Microfiber Encapsulated Probiotic: Viability, Acid and Bile Tolerance during Storage at Different Temperature

et al. 2022

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This work aimed to analyze the physicochemical properties of cellulose from OPT used in the fabrication of CMF and evaluate the efficacy of the hydrogel CMF as an encapsulant for L. fermentum InaCC B1295 stored at room temperature and in the refrigerator. The Kjeldahl method was used to evaluate the protein content; the gravimetric method was used to determine OPT's ash, moisture, and fiber contents; the Soxhlet method was used to determine the fat content carbohydrates were computed using the difference method. The levels of holocellulose, lignin, and cellulose were also determined. Viability, acid and bile resistance of strain B1295 were evaluated at various temperatures for 35 days. The most abundant component of OPT fiber was cellulose, followed by hemicellulose and lignin. XRD examination revealed that OPT cellulose has a crystal index of 83.40%. FTIR analysis was used to detect the stretching vibrations of the –OH group on cellulose at 3419.03 cm-1. CMF hydrogel from OPT sustained L. fermentum InaCC B1295 survival for up to 28 days at room and refrigerated temperatures. At acidic conditions and in the presence of bile, the viability of L. fermentum InaCC B1295 was excellent, with a drop in cell population of less than 0.2 log CFU/g over 35 days at room and refrigerated temperatures. CMF obtained from OPT can be used as an encapsulant to maintain viability, acid resistance and bile of probiotics. There is still a need for research into the usage of CMF from OPT in combination with other encapsulants to extend the storage life of L. fermentum InaCC B1295. Doi: 10.28991/ESJ-2022-06-01-08 Full Text: PDF

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Section: -2-viability Of Strain B1295supporting

confidence: 89%

Cellulose Microfiber Encapsulated Probiotic: Viability, Acid and Bile Tolerance during Storage at Different Temperature

et al. 2022

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“…This study is also in accordance with the research results by Ref. [19], which utilizes sodium alginate as a coating. The results showed that the viability of encapsulated cells was better than cells without encapsulation during 8 weeks of frozen storage, with a decreased viability of Lactobacillus plantarum and Streptococcus thermopillus without encapsulation decreased by 12% and 25%, respectively.…”

Section: Viability Of Lactobacillus Fermentum Inacc B1295 Bacteriasupporting

confidence: 92%

“…This decrease is due to the longer storage time, the MCC hydrogel layer on the encapsulant that envelops bacterial cells has decreased stability so that the bonds that occur between MCC begin to stretch and bacteria quickly come out of the dressing, which results in bacteria experiencing direct contact with an acidic environment that causes cell damage to bacteria and affects bacterial growth. The results of this study agree with [19], which states that the robustness of the encapsulant matrix formed will decrease with the length of storage.…”

Section: Total Bacteria Lactobacillus Fermentumsupporting

confidence: 90%

“…This follows research by Ref. [19], which utilized sodium alginate as an encapsulant. The results showed that the encapsulant was resistant and could protect LAB from freezing temperatures for 8 weeks of storage with a decrease in Lactobacillus plantarum and Streptococcus thermopillus of only 2% and 14%, respectively.…”

Section: Decrease Of Lactobacillus Fermentum Inacc B1295 Against Phmentioning

confidence: 99%

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Resistance of Lactobacillus fermentum InaCC B1295 Encapsulated in Microcrystalline Cellulose from Palm Leaf Waste to Acidic Conditions Across Various Temperatures and Storage Durations"

Firdaus,

Kasim,

Kasim

2024

ajarcde

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Lactobacillus fermentum InaCC B1295 is a probiotic bacterium with limited tolerance to acidic environments, bile fluids, and high temperatures, necessitating physical protection via microcrystalline cellulose (MCC) encapsulation derived from palm leaf waste. This study aimed to evaluate the resistance of encapsulated probiotic bacteria under acidic conditions at various temperatures and storage durations to identify the optimal storage temperature. A factorial complete randomized design (CRD) with two factors was employed: storage temperature (room temperature, 4°C, and -18°C) and storage time (0, 7, 14, 21, 28, 35, and 42 days). The results indicated that storage time and the interaction between storage time and temperature significantly affected the total lactic acid bacteria (LAB) count. However, temperature alone and its interaction with storage time did not significantly impact the percentage reduction in the number of encapsulated Lactobacillus fermentum InaCC B1295 at pH 2. Overall, encapsulated bacteria stored at various temperatures demonstrated comparable bacterial resistance..

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“…Bacteria undergo a coating process by a polymer wall layer. The advantage of encapsulation is that it has a semipermeable and strong membrane so that bacterial cells can withstand extreme conditions (Samedi & Charles, 2019;Haffner et al, 2016;Bilang et al, 2018;Kamil et al, 2020;Trimudita, 2021).…”

Section: Resultsmentioning

confidence: 99%

Viability of Lactobacillus bulgaricus and Streptococcus thermophilus in Encapsulated Probiotic Candy With Freeze-Dry Method

Sutandi

Haryanto²,

Kusumawati

2022

sceducatia

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The level of viable cell count in probiotic candy, based on WHO standard is >10 6 CFU/ml or >10 6 CFU/g. Meanwhile, information on the bacterial viability of probiotic candy according to WHO standards is still limited. The experimental study was conducted to discover the viability of Lactobacillus bulgaricus and Streptococcus thermophilus in encapsulated probiotic candy. Encapsulants consisted of maltodextrin, gum, corn starch, and skim milk. Encapsulation of probiotic bacteria used freeze-dry method. Probiotic candies were stored at room temperature for 14 days in an aerobic condition. The viability of probiotics candy was tested and the results showed significance for each type of encapsulation (p<0.05). The gum and corn starch encapsulations showed bacteria viability that met WHO standards for functional foods, which ranged from 20,333,333±7,637,626 to 31,553,333±2,741,894 CFU/g (>10 6 CFU/g). The most preferred encapsulant in terms of taste, texture, and aroma was identified as skim milk encapsulation. Further research on the long-term storage of probiotic candy and the viability of probiotic candy bacteria in the gastrointestinal tract (in vivo) is needed.

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Mempelajari Viabilitas Enkapsulasi Sel Probiotik (Lactobacillus Plantarum Dan Streptococcus Thermophilus) Pada Es Krim

Cited by 4 publications

References 7 publications

Cellulose Microfiber Encapsulated Probiotic: Viability, Acid and Bile Tolerance during Storage at Different Temperature

Cellulose Microfiber Encapsulated Probiotic: Viability, Acid and Bile Tolerance during Storage at Different Temperature

Resistance of Lactobacillus fermentum InaCC B1295 Encapsulated in Microcrystalline Cellulose from Palm Leaf Waste to Acidic Conditions Across Various Temperatures and Storage Durations"

Viability of Lactobacillus bulgaricus and Streptococcus thermophilus in Encapsulated Probiotic Candy With Freeze-Dry Method

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