Menaquinone composition in the classification and identification of aerobic actinomycetes

Yassin, Atteyet-Alla Fetouh; Brzezinka, H.; Schaal, K. P.; Trüper, Hans G.; Pulverer, G.

doi:10.1016/s0176-6724(88)80050-6

Cited by 6 publications

(2 citation statements)

References 42 publications

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“…The main source of ATP in aerobically growing organisms is oxidative phosphorylation. Streptomyces are known to use menaquinone as an electron transporter in the membrane (Pandya and King 1966;Yassin et al 1988), even though surprisingly enough the genes for menaquinone synthesis were not found in the sequenced genomes of S. coelicolor A3 (2) and S. avermitilis. The stoichiometry of the electron transport chain was assumed to be the same as recently described in the actinomycete Corynebacterium glutamicum (Bott and Niebisch 2003).…”

Section: Metabolic Model Of S Coelicolormentioning

confidence: 99%

Genome-scale analysis of Streptomyces coelicolor A3(2) metabolism

Borodina¹,

Krabben²,

Nielsen³

2005

Genome Res.

228

198

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Streptomyces are filamentous soil bacteria that produce more than half of the known microbial antibiotics. We present the first genome-scale metabolic model of a representative of this group-Streptomyces coelicolor A3(2). The metabolism reconstruction was based on annotated genes, physiological and biochemical information. The stoichiometric model includes 819 biochemical conversions and 152 transport reactions, accounting for a total of 971 reactions. Of the reactions in the network, 700 are unique, while the rest are iso-reactions. The network comprises 500 metabolites. A total of 711 open reading frames (ORFs) were included in the model, which corresponds to 13% of the ORFs with assigned function in the S. coelicolor A3(2) genome. In a comparative analysis with the Streptomyces avermitilis genome, we showed that the metabolic genes are highly conserved between these species and therefore the model is suitable for use with other Streptomycetes. Flux balance analysis was applied for studies of the reconstructed metabolic network and to assess its metabolic capabilities for growth and polyketides production. The model predictions of wild-type and mutants' growth on different carbon and nitrogen sources agreed with the experimental data in most cases. We estimated the impact of each reaction knockout on the growth of the in silico strain on 62 carbon sources and two nitrogen sources, thereby identifying the "core" of the essential reactions. We also illustrated how reconstruction of a metabolic network at the genome level can be used to fill gaps in genome annotation.

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Section: Metabolic Model Of S Coelicolormentioning

confidence: 99%

Genome-scale analysis of Streptomyces coelicolor A3(2) metabolism

Borodina¹,

Krabben²,

Nielsen³

2005

Genome Res.

228

198

View full text Add to dashboard Cite

show abstract

“…pradimicin B (V) [7], and all the complex N-heterocyclics with more than three rings, such as the saframycins. In contrast to these quinones of very varied structure the menaqumones form a homogeneous group in Nocardia, Mycobacteria, Saccharothrix and other genera which are of value in the classification of aerobic Actinomycetes [8]. Structure (VI; R=Me or H) shows the general type, individual quinones differing in chain length (n = 4-12), in degree of side-chain unsaturation, and occasional methylation in the aromatic ring.…”

Section: Bacteriamentioning

confidence: 99%