Mendelian transmission, transgene dosage and growth phenotype in transgenic tilapia (Oreochromis hornorum) showing ectopic expression of homologous growth hormone

Increased food intake in growth hormone-transgenic common carp (Cyprinus carpio L.) may be mediated by upregulating Agouti-related protein (AgRP)

“…To date, several stable lines of growth-enhanced transgenic fishes have been generated, including Atlantic salmon (Salmo salar) (Fletcher et al, 2004), coho salmon (Oncorhynchus kisutch) (Devlin et al, 2004), mud loach (Misgurnus mizolepis) (Nam et al, 2002), tilapia (Oreochromis sp.) (Martinez et al, 1999;Rahman et al, 1998) and common carp (Cyprinus carpio L.) (Wang et al, 2001;Zhong et al, 2012). During recent years, it has become clear that elevated growth hormone (GH) levels in transgenic fish induce a wide range of effects apart from growth promotion (Devlin et al, 1994;Du et al, 1992;Martinez et al, 1996;Nam et al, 2001;Rahman et al, 1998;Wang et al, 2001;Zhong et al, 2012), including altered metabolism (Guan et al, 2008;McKenzie et al, 2003;Stevens et al, 1998), swimming performance (Farrell et al, 1997;Lee et al, 2003;Stevens et al, 1998), anti-predator behavior (Abrahams and Sutterlin, 1999;Duan et al, 2010;Dunham et al, 1999) and growth-related neuroendocrine regulation (Raven et al, 2008).…”

Tilapia chromosomal growth hormone gene expression accelerates growth in transgenic zebrafish (Danio rerio)

“…Furthermore, in our construct we included the chromosomal tiGH gene. In previous experiments we have shown that the expression of the tiGH cDNA in transgenic tilapia results in accelerated growth (Martínez et al 1996;Martínez et al 1999) while the inclusion of fishderived intron sequences in chimeric constructs increases transgene expression in zebrafish embryos (García del Barco et al 1994). Both elements are resumed in the chrtiGH gene.…”

The use of genetic engineering techniques to improve the lipid composition in meat, milk and fish products: a review

“…Sugiyama et al (2012) reported a significant beneficial influence of growth hormone transgenesis on liver fatty-acid composition in Amago salmon (Oncorhynchus masou ishikawae); thus transgenic fish had decreased contents of SFAs and MUFAs, as well as an increased content of PUFAs, among others EPA and DPA. The impact of an enhanced metabolism rate on lipid changes was also evident in the case of a transgenic tilapia (Oreochromis hornorum) containing foreign growth hormone, with a significantly reduced cholesterol concentration in its blood serum Martínez et al (1999). According to the authors, this effect of transgenesis can be linked to an increase in the use of cholesterol in the process of cell membrane synthesis, occurring due to the accelerated rate of mitotic cell division in growth-enhanced fish Martínez et al (1999).…”

“…The impact of an enhanced metabolism rate on lipid changes was also evident in the case of a transgenic tilapia (Oreochromis hornorum) containing foreign growth hormone, with a significantly reduced cholesterol concentration in its blood serum Martínez et al (1999). According to the authors, this effect of transgenesis can be linked to an increase in the use of cholesterol in the process of cell membrane synthesis, occurring due to the accelerated rate of mitotic cell division in growth-enhanced fish Martínez et al (1999).…”