Meningitis Due to Mixed Infection with Penicillin-Resistant and Penicillin-Susceptible Strains of
            <i>Streptococcus pneumoniae</i>

Cháves, Fernando; Campelo, Carolina; Sanz, Francisca; Otero, Joaquín R.

doi:10.1128/jcm.41.1.512-513.2003

Cited by 9 publications

(11 citation statements)

References 9 publications

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“…Although the extent of simultaneous carriage of different serotypes is not known, a limitation of this study, as well as most others reporting pneumococcal incidence data, is the possibility of simultaneous carriage of more than one pneumococcal serotype (Austrian, 1981(Austrian, , 1986Chaves et al, 2003). However, the methods used in this study and the data resulting from it compare well with others.…”

Section: Discussionmentioning

confidence: 73%

Pneumococci causing invasive disease in children prior to the introduction of pneumococcal conjugate vaccine in Scotland

Clarke

Jefferies

Smith³

et al. 2006

Journal of Medical Microbiology

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This study aimed to determine the serotypes and sequence types (STs) of pneumococci causing paediatric invasive disease in Scotland prior to the introduction of pneumococcal conjugate vaccines (PCVs). All invasive pneumococci isolated between 2000 and 2004 from children aged less than 5 years in Scotland were used. The isolates were characterized by serotyping and multi-locus sequence typing. Two hundred and seventeen pneumococci were characterized into 22 different serogroups/types, the most common, in rank order, being 14, 19F, 6B, 18C, 23F, 9V, 4, 1, 19A and 6A. They were further genotyped into 77 different STs, the three most common being 9, 162 and 176. Common serotypes possessed multiple STs, but pneumococci of a particular clone were mostly associated with a particular serotype. The seven most common serotypes are included in the 7-valent polysaccharide conjugate vaccine (PCV7). Serotype coverage for PCV7 was 76.5 % in those aged less than 5 years but increased to 88.9 % for those aged 1 year. The introduction of PCV7 into the childhood immunization schedule would reduce the burden of pneumococcal disease in children, although continued surveillance of invasive pneumococcal disease will be required before, during and after the introduction of PCVs.

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Section: Discussionmentioning

confidence: 73%

Pneumococci causing invasive disease in children prior to the introduction of pneumococcal conjugate vaccine in Scotland

Clarke

Jefferies

Smith³

et al. 2006

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…One limitation of this study, as well as most others which report pneumococcal incidence data, is the possibility of simultaneous carriage of more than one pneumococcal serotype (3,4,9). The extent of simultaneous carriage of different serotypes is not known.…”

Section: Discussionmentioning

confidence: 99%

Potential Impact of Conjugate Vaccine on the Incidence of Invasive Pneumococcal Disease among Children in Scotland

Clarke

Jefferies

Smith³

et al. 2006

J Clin Microbiol

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We sought to determine the potential impact of seven-valent pneumococcal conjugate vaccine on the incidence of invasive pneumococcal disease (IPD) among children in Scotland. Invasive pneumococci from blood and cerebrospinal fluid, isolated between 2000 and 2004 from all children aged less than 5 years in Scotland, were characterized by serotyping. Using reported efficacy data of the seven-valent pneumococcal conjugate vaccine (PCV7) along with likely coverage rates, we made an estimation of the potential impact on the incidence of IPD among children in Scotland. A total of 217 pneumococci were characterized into 22 different serogroups/types, the most common, in rank order, being 14, 19F, 6B, 18C, 23F, 9V, 4, 1, 19A, and 6A. Estimated serotype coverage for PCV7 was 76.5% in those aged less than 5 years of age but increased to 88.9% for those aged 1 year. By using serotype coverage and estimates of vaccine efficacy and uptake, the potential impact of the vaccine for those greater than 2 months of age, but less than 5 years, was estimated as 67.3%, leading to an average of 29 preventable cases per year.

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“…Although these organisms share an almost identical partial rpoB gene sequence, they do exhibit three distinct mutations in the rpoB sequence that could serve as a molecular signature for their accurate identification. In addition to S. pneumoniae (8), S. agalactiae is a leading cause of bacterial meningitis, particularly in neonates, a condition requiring rapid and accurate etiological diagnosis. With respect to this goal, rpoB exhibited Ͼ15% sequence divergence, a value leading to unambiguous species identification.…”

Section: Discussionmentioning

confidence: 99%

rpoB Gene Sequence-Based Identification of Aerobic Gram-Positive Cocci of the Genera Streptococcus , Enterococcus , Gemella , Abiotrophia , and Granulicatella

et al. 2004

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We developed a new molecular tool based on rpoB gene (encoding the beta subunit of RNA polymerase) sequencing to identify streptococci. We first sequenced the complete rpoB gene for Streptococcus anginosus, S. equinus, and Abiotrophia defectiva. Sequences were aligned with these of S. pyogenes, S. agalactiae, and S. pneumoniae available in GenBank. Using an in-house analysis program (SVARAP), we identified a 740-bp variable region surrounded by conserved, 20-bp zones and, by using these conserved zones as PCR primer targets, we amplified and sequenced this variable region in an additional 30 Streptococcus, Enterococcus, Gemella, Granulicatella, and Abiotrophia species. This region exhibited 71.2 to 99.3% interspecies homology. We therefore applied our identification system by PCR amplification and sequencing to a collection of 102 streptococci and 60 bacterial isolates belonging to other genera. Amplicons were obtained in streptococci and Bacillus cereus, and sequencing allowed us to make a correct identification of streptococci. Molecular signatures were determined for the discrimination of closely related species within the S. pneumoniae-S. oralis-S. mitis group and the S. agalactiae-S. difficile group. These signatures allowed us to design a S. pneumoniae-specific PCR and sequencing primer pair.

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Meningitis Due to Mixed Infection with Penicillin-Resistant and Penicillin-Susceptible Strains of Streptococcus pneumoniae

Cited by 9 publications

References 9 publications

Pneumococci causing invasive disease in children prior to the introduction of pneumococcal conjugate vaccine in Scotland

Pneumococci causing invasive disease in children prior to the introduction of pneumococcal conjugate vaccine in Scotland

Potential Impact of Conjugate Vaccine on the Incidence of Invasive Pneumococcal Disease among Children in Scotland

rpoB Gene Sequence-Based Identification of Aerobic Gram-Positive Cocci of the Genera Streptococcus , Enterococcus , Gemella , Abiotrophia , and Granulicatella

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