Meningitis e hipoacusia por manipulación de carne porcina

Moraga, Juan; Fíca, Alberto; Navarrete, Braulio; Henríquez, Ruby; Navarrete-Asenjo, Claudio

doi:10.4067/s0716-10182018000600731

Cited by 2 publications

(2 citation statements)

References 3 publications

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“…Streptococcus pneumoniae, Haemophilus influenzae and Neisseria are the main bacterial pathogens responsible for acute bacterial meningitis (9,10) and it is reported that the host genetic factors are the major determinants of susceptibility for bacterial meningitis (7). Recently, zoonotic meningitis has been occasionally linked to pig farmers who have developed acute bacterial meningitis after handling raw swine meat (11). At present, there remains an incomplete understanding of the pathogenesis and the best methods for the prevention and treatment of bacterial meningitis and further research looking at the pathogenesis of bacterial meningitis is urgently needed.…”

Section: Introductionmentioning

confidence: 99%

Methylome and Transcriptome-Based Integration Analysis Identified Molecular Signatures Associated With Meningitis Induced by Glaesserella parasuis

Li-wei

Chen

et al. 2022

Front. Immunol.

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Glaesserella parasuis (G. parasuis) can elicit serious inflammatory responses and cause meningitis in piglets. Previous epigenetic studies have indicated that alterations in host DNA methylation may modify the inflammatory response to bacterial infection. However, to date, genome-wide analysis of the DNA methylome during meningitis caused by G. parasuis infection is still lacking. In this study, we employed an unbiased approach using deep sequencing to profile the DNA methylome and transcriptome from G. parasuis infected porcine brain (cerebrum) and integrated the data to identify key differential methylation regions/sites involved in the regulation of the inflammatory response. Results showed that DNA methylation patterns and gene expression profiles from porcine brain were changed after G. parasuis infection. The majority of the altered DNA methylation regions were found in the intergenic regions and introns and not associated with CpG islands, with only a low percentage occurring at promoter or exon regions. Integrated analysis of the DNA methylome and transcriptome identified a number of inversely and positively correlated genes between DNA methylation and gene expression, following the criteria of |log2FC| > 0.5, |diffMethy| > 0.1, and P < 0.05. Differential expression and methylation of two significant genes, semaphoring 4D (SEMA4D) and von Willebrand factor A domain containing 1 (VWA1), were validated by qRT-PCR and bisulfite sequencing. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses demonstrated that DNA methylation inversely correlated genes in G. parasuis infected porcine brains were mainly involved with cell adhesion molecules (CAMs), bacterial invasion of epithelial cells, RIG-1-like receptor signaling pathways, and hematopoietic cell lineage signaling pathways. In addition, a protein-protein interaction network of differentially methylated genes found potential candidate molecular interactions relevant to the pathology of G. parasuis infection. To the best of our knowledge, this is the first attempt to integrate the DNA methylome and transcriptome data from G. parasuis infected porcine brains. Our findings will help understanding the contribution of genome-wide DNA methylation to the pathogenesis of meningitis in pigs and developing epigenetic biomarkers and therapeutic targets for the treatment of G. parasuis induced meningitis.

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Section: Introductionmentioning

confidence: 99%

Methylome and Transcriptome-Based Integration Analysis Identified Molecular Signatures Associated With Meningitis Induced by Glaesserella parasuis

Li-wei

Chen

et al. 2022

Front. Immunol.

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“…Infection in humans presents clinically as meningitis, endocarditis, septicemia, and/or arthritis, and thus far is primarily reported in Asia [3,4]. It is rarely observed in western regions, such as Argentina and the United States [5,6].…”

Section: Introductionmentioning

confidence: 99%

Meningitis due to Streptococcus suis in Two Patients with Occupational Exposure from Northeastern Brazil

Matos

Farias

Dias

et al. 2021

Case Reports in Infectious Diseases

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Streptococcus suis is an emerging zoonotic agent that causes bacterial meningitis. S. suis is an encapsulated Gram-positive, facultative anaerobic bacterium. This infection usually manifests in humans as meningitis, endocarditis, septicemia, and/or arthritis. The primary groups at risk for S. suis infection are individuals occupationally exposed to pigs and/or pork, for example, farmers, butchers, and hunters. Herein, we report two cases of meningitis related to S. suis with occupational exposure from the Ceará state, northeastern Brazil.

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Meningitis e hipoacusia por manipulación de carne porcina

Cited by 2 publications

References 3 publications

Methylome and Transcriptome-Based Integration Analysis Identified Molecular Signatures Associated With Meningitis Induced by Glaesserella parasuis

Methylome and Transcriptome-Based Integration Analysis Identified Molecular Signatures Associated With Meningitis Induced by Glaesserella parasuis

Meningitis due to Streptococcus suis in Two Patients with Occupational Exposure from Northeastern Brazil

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