Meniskusdegenerationsscore und NITEGE-Expression

Krenn, Veit; Knöß, P.; Rüther, W.; Jakobs, Martin; Otto, Mike; Krukemeyer, Manfred Georg; Heine, Alexander; Möllenhoff, G.; Kurz, Bodo

doi:10.1007/s00132-010-1606-4

Cited by 24 publications

(8 citation statements)

References 23 publications

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“…In some slides of meniscal tissue after ACLR additional pronounced mucoid changes which are characterized by less stained and overstained areas (circle in Fig 2D ) in accordance with separation and the formation of tears and multiple obvious matrix clefts have been seen mainly in the PI ( Fig 2D ) but also in the AH (Grade 3). Other criteria for high-grade degeneration that have been described previously [ 38 , 39 ] such as polymorphism of chondrocytes and fat accumulations were rare in the menisci of the current study. The lateral meniscus after ACLR showed none of the degenerative changes of this type.…”

Section: Resultsmentioning

confidence: 47%

A Porcine Animal Model for Early Meniscal Degeneration – Analysis of Histology, Gene Expression and Magnetic Resonance Imaging Six Months after Resection of the Anterior Cruciate Ligament

Kreinest

Reisig

Ströbel³

et al. 2016

PLoS ONE

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Background/ObjectiveThe menisci of the mammalian knee joint balance the incongruence between femoral condyle and tibial plateau and thus menisci absorb and distribute high loads. Degeneration processes of the menisci lead to pain syndromes in the knee joint. The origin of such degenerative processes on meniscal tissue is rarely understood and may be described best as an imbalance of anabolic and catabolic metabolism. A standardized animal model of meniscal degeneration is needed for further studies. The aim of the current study was to develop a porcine animal model with early meniscal degeneration.Material and MethodsResection of the anterior cruciate ligament (ACLR) was performed on the left knee joints of eight Göttingen minipigs. A sham operation was carried out on the right knee joint. The grade of degeneration was determined 26 weeks after the operation using histology and magnetic resonance imaging (MRI). Furthermore, the expression of 14 genes which code for extracellular matrix proteins, catabolic matrix metalloproteinases and inflammation mediators were analyzed.ResultsDegenerative changes were detected by a histological analysis of the medial meniscus after ACLR. These changes were not detected by MRI. In terms of their gene expression profile, these degenerated medial menisci showed a significantly increased expression of COL1A1.ConclusionThis paper describes a new animal model for early secondary meniscal degeneration in the Göttingen minipig. Histopathological evidence of the degenerative changes could be described. This early degenerative changes could not be seen by NMR imaging.

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Section: Resultsmentioning

confidence: 47%

A Porcine Animal Model for Early Meniscal Degeneration – Analysis of Histology, Gene Expression and Magnetic Resonance Imaging Six Months after Resection of the Anterior Cruciate Ligament

Kreinest

Reisig

Ströbel³

et al. 2016

PLoS ONE

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“…Human meniscal degeneration has also been described by several authors [18, 20, 21, 24, 25, 31, 32, 35–37, 39, 44–49]. However, most of the grading systems for meniscus pathology are MRI-based, and describe tears and grades of mucoid degeneration [19, 50–53].…”

Section: Discussionmentioning

confidence: 99%

“…Similar accepted systems for macroscopic and histologic assessment of meniscus are not available. Most studies of meniscus evaluation systems concentrated on narrow features of interest such as cellularity and matrix organization [11, 18–21], cellular phenotype [22, 23] or meniscal tears [24, 25]. However, no scoring approach integrates the status of the tissue surface, cells, tissue morphology and histochemical analyses to characterize normal aging and diseased tissue at both macroscopic and microscopic levels.…”

Section: Introductionmentioning

confidence: 99%

Macroscopic and histopathologic analysis of human knee menisci in aging and osteoarthritis

Pauli

Grogan

Patil

et al. 2011

Osteoarthritis and Cartilage

318

306

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Objective Meniscus lesions following trauma or associated with osteoarthritis (OA) have been described, yet meniscus aging has not been systematically analyzed. The objectives of this study were to (i) establish standardized protocols for representative macroscopic and microscopic analysis, (ii) improve existing scoring systems, and (iii) apply these techniques to a large number of human menisci. Design Medial and lateral menisci from 107 human knees were obtained and cut in two different planes (triangle/crossection and transverse/horizontal) in three separate locations (mid portion, anterior and posterior horns). All sections included vascular and avascular regions and were graded for i) surface integrity, ii) cellularity, iii) matrix/fiber organization and collagen alignment, and iv) Safranin-O staining intensity. The cartilage in all knee compartments was also scored. Results The new macroscopic and microscopic grading systems showed high inter-reader and intra-reader intraclass correlation coefficients. The major age-related changes in menisci in joints with no or minimal OA included increased Safranin-O staining intensity, decreased cell density, the appearance of acellular zones, and evidence of mucoid degeneration with some loss of collagen fiber organization. The earliest meniscus changes occurred predominantly along the inner rim. Menisci from OA joints showed severe fibrocartilaginous separation of the matrix, extensive fraying, tears and calcification. Abnormal cell arrangements included decreased cellularity, diffuse hypercellularity along with cellular hypertrophy and abnormal cell clusters. In general, the anterior horns of both medial and lateral menisci were less affected by age and OA. Conclusions New standardized protocols and new validated grading systems allowed us to conduct a more systematic evaluation of changes in aging and OA menisci at a macroscopic and microscopic level. Several meniscus abnormalities appear to be specific to aging in the absence of significant OA. With aging the meniscal surface can be intact but abnormal matrix organization and cellularity was observed within the meniscal substance. The increased Safranin-O staining appears to represent a shift from fibroblastic to chondrocytic phenotype during aging and early degeneration.

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“…Sections of the ALH were stained with hematoxylin and eosin (Fig. 2b ) and samples of the FH were stained with 1% Safranin-O to evaluate the histological degeneration grade of the tissue sample according to the Krenn-score for fibrocartilage (grade 0–3; Table 2 ) [ 32 ] and the Osteoarthritis Research Society International (OARSI) osteoarthritis cartilage histopathology assessment system for hyaline cartilage (grade 0–6) [ 33 ]. Calcifications identified by DCR were confirmed to represent calcium-phosphate crystal deposition by von Kossa staining (Fig.…”

Section: Methodsmentioning

confidence: 99%

Calcification of the acetabular labrum of the hip: prevalence in the general population and relation to hip articular cartilage and fibrocartilage degeneration

et al. 2018

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BackgroundMeniscal calcification is considered to play a relevant role in the pathogenesis of osteoarthritis of the knee. Little is known about the biology of acetabular labral disease and its importance in hip pathology. Here, we analyze for the first time the calcification of the acetabular labrum of the hip (ALH) and its relation to hip cartilage degeneration.MethodsIn this cross-sectional post-mortem study of an unselected sample of the general population, 170 ALH specimens and 170 femoral heads from 85 donors (38 female, 47 male; mean age 62.1 years) were analyzed by high-resolution digital contact radiography (DCR) and histological degeneration grade. The medial menisci (MM) from the same 85 donors served as an intra-individual reference for cartilage calcification (CC). Scanning electron microscopy (SEM), energy dispersive analysis (ED) and Raman spectroscopy were performed for characterization of ALH CC.ResultsThe prevalence of CC in the ALH was 100% and that in the articular cartilage of the hip (ACH) was 96.5%. Quantitative analysis revealed that the amount of ALH CC was higher than that in the ACH (factor 3.0, p < 0.001) and in the MM (factor 1.3, p < 0.001). There was significant correlation between the amount of CC in the fibrocartilage of the left and right ALH (r = 0.70, p < 0.001). Independent of age, the amount of ALH CC correlated with histological degeneration of the ALH (Krenn score) (r = 0.55; p < 0.001) and the ACH (Osteoarthritis Research Society International (OARSI), r = 0.69; p < 0.001). Calcification of the ALH was characterized as calcium pyrophosphate dihydrate deposition.ConclusionThe finding that ALH fibrocartilage is a strongly calcifying tissue is unexpected and novel. The fact that ALH calcification correlates with cartilage degeneration independent of age is suggestive of an important role of ALH calcification in osteoarthritis of the hip and renders it a potential target for the prevention and treatment of hip joint degeneration.

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Meniskusdegenerationsscore und NITEGE-Expression

Abstract: The thoroughly defined degeneration score (grade 1 - grade 3 MD) is suitable to assess the severity of degeneration. Extracellular NITEGE deposits can be regarded as an immunohistochemical marker for advanced (grades 2 and 3) MD.

Cited by 24 publications

References 23 publications

A Porcine Animal Model for Early Meniscal Degeneration – Analysis of Histology, Gene Expression and Magnetic Resonance Imaging Six Months after Resection of the Anterior Cruciate Ligament

A Porcine Animal Model for Early Meniscal Degeneration – Analysis of Histology, Gene Expression and Magnetic Resonance Imaging Six Months after Resection of the Anterior Cruciate Ligament

Macroscopic and histopathologic analysis of human knee menisci in aging and osteoarthritis

Calcification of the acetabular labrum of the hip: prevalence in the general population and relation to hip articular cartilage and fibrocartilage degeneration

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