ObjectivesThe objectives of the HOLISTIC Cohort Study are to establish a 3-year prospective cohort study that characterizes the health of students within and across health professionals’ education programs during the coronavirus disease 2019 (COVID-19) pandemic, implement an interprofessional student research team, and inform initiatives to improve student health. This report describes the rationale and design of the HOLISTIC Cohort Study, including recruitment strategy, survey development, data management, and descriptive statistics of the first wave of study participants.MethodsAn interprofessional student research team was formed to continuously inform study design. The first wave of recruitment was conducted from April 14, 2021 to May 5, 2021 across seven health science colleges (applied health, dentistry, medicine, nursing, pharmacy, public health, social work) at the University of Illinois Chicago in Chicago, IL. Eligible students were sent an invitation via email to complete an online survey after providing electronic informed consent. The online survey was based on the U.S. Centers for Disease Control and Prevention’s Behavioral Risk Factor Surveillance System 2019 survey and the 2014 World Health Organization Report of the Strategic Advisory Group of Experts Working Group Questionnaire. Two additional recruitment waves are planned in the Spring 2022 and Spring 2023; follow-up of participants previously enrolled will occur during these second and third recruitment waves.ResultsOf 5,118 students invited to participate in the first wave, 553 (10.8%) completed the survey and includes participants from all seven health science colleges. The average age of participants is 27.3 years, 435 (78.8%) identify as female, and 137 (24.8%) identify as an underrepresented minority. Overall, 465 (84.6%) participants reported being currently employed for wages. Just over half (51%) reported no days with poor physical health within a month but only 11.2% reported no days with poor mental health within a month. Nearly one in ten (9.4%) reported having ever had a positive test for COVID-19.ConclusionThe HOLISTIC Cohort Study of health professional students across seven health science colleges has completed the first of three waves of enrollment during the COVID-19 pandemic. Based on the first wave of study participants, increased attention to supporting the mental and physical health of health professional students is needed.