Menthol-assisted homogenous liquid-liquid microextraction for HPLC/UV determination of favipiravir as an antiviral for COVID-19 in human plasma

Abdallah, Inas A.; Hammad, Sherin F.; Bedair, Alaa; Mansour, Fotouh R.

doi:10.1016/j.jchromb.2021.123087

Cited by 37 publications

(19 citation statements)

References 34 publications

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Section: Introductionmentioning

confidence: 99%

“…After a literature survey, It was found that few analytical methods were reported for estimation of FVP in different matrices using different techniques; spectrophotometric (BULDUK, 2021 ), spectrofluorimetric (Mikhail et al 2021 ) chromatographic (Abdallah et al 2022 ; BULDUK, 2021 ; I. A. Abdallah, 2022 ; Kaddah et al 2021 ; Mikhail et al 2021 ; M. I. Morsy et al 2021 ; Mosaad I. Morsy et al 2021 ), and voltametric methods (Mehmandoust et al 2021 ). Although few stability-indicating HPLC methods were previously reported, no comprehensive stability-indicating method with isolation and characterization of the degradation products has been published yet for the quantification of FVP.…”

Section: Introductionmentioning

confidence: 99%

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The anti-COVID-19 drug Favipiravir: Degradation, Method development, Validation, NMR/LC–MS characterization, and In-vitro safety evaluation

et al. 2022

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It is critical to characterize the degradation products of therapeutic drugs to determine their safety as these degradation products may possess fatal effects on the human physiological system. Favipiravir (FVP), a novel anti-Covid-19 drug, that is recently used all over the world with a great impact on humanity was our target to explore more about its toxicity, the margins of its safety, and its degradants in different degradation conditions. The goal of this study is to identify, characterize, and confirm the structures of FVP oxidative and alkaline breakdown products, as well as to assess their safety utilizing in-vitro SRB cytotoxicity assay on normal human skin fibroblasts (NHSF) cell lines. After oxidative and alkaline degradation of FVP, one degradation product was produced in each condition which was isolated from FVP using flash chromatography, characterized by 1HNMR and LC–MS/MS techniques. A reversed-phase Thermo Fischer Hypersil C18 column (4.6 × 150 mm, 5 m) was used to achieve HPLC chromatographic separation. Acetonitrile-5 mM potassium dihydrogen phosphate (pH 2.5) (50:50, v/v) was employed as the mobile phase, with a flow rate of 1 mL/min. At 332 nm, the column effluent was measured. Over the concentration range of 0.5–100 µg/mL, the calibration curve was linear. The intra-day and inter-day relative standard deviations were less than 2%, and good percentage recoveries were obtained that fulfilled the acceptance criteria of the International Conference on Harmonization (ICH) recommendations. The Plackett–Burman design was used to assess the robustness. Each degradant was isolated single using Flash chromatography and methylene chloride: methanol gradient mobile phase. The chemical structures of the degradation products have been confirmed and compared to the intact FVP using 1H-NMR, and Mass spectroscopy. A postulated mechanism of the degradation process has been depicted and the degradants fragmentation pattern has been portrayed. In addition, the in vitro SRB cytotoxicity assay to evaluate the safety profile of FVP and the degradation end products showed their high safety margin in both conditions with IC50 ˃100 µg/ml with no signs of toxicity upon examination of the treated NHSF cells under the optical microscope

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The anti-COVID-19 drug Favipiravir: Degradation, Method development, Validation, NMR/LC–MS characterization, and In-vitro safety evaluation

et al. 2022

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“…Several analytical methods for FAV determination are commercially available, including high-performance liquid chromatography (HPLC) [ 5 , 6 ], spectrometric [ 7 ], and spectrofluorimetric methods [ 8 ]. Despite the significant advances that have been made by the above traditional approaches, a portable and reliable method for selective determination in the laboratory and environment still faces substantial challenges such as multiple interferences and personal care products, high cost, and the need for complicated and expensive instruments.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Sensing of Favipiravir with an Innovative Water-Dispersible Molecularly Imprinted Polymer Based on the Bimetallic Metal-Organic Framework: Comparison of Morphological Effects

2022

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Molecularly imprinted polymers (MIPs) are widely used as modifiers in electrochemical sensors due to their high sensitivity and promise of inexpensive mass manufacturing. Here, we propose and demonstrate a novel MIP-sensor that can measure the electrochemical activity of favipiravir (FAV) as an antiviral drug, thereby enabling quantification of the concentration of FAV in biological and river water samples and in real-time. MOF nanoparticles’ application with various shapes to determine FAV at nanomolar concentrations was described. Two different MOF nanoparticle shapes (dodecahedron and sheets) were systematically compared to evaluate the electrochemical performance of FAV. After carefully examining two different morphologies of MIP-Co-Ni@MOF, the nanosheet form showed a higher performance and efficiency than the nanododecahedron. When MIP-Co/Ni@MOF-based and NIP-Co/Ni@MOF electrodes (nanosheets) were used instead, the minimum target concentrations detected were 7.5 × 10−11 (MIP-Co-Ni@MOF) and 8.17 × 10−9 M (NIP-Co-Ni@MOF), respectively. This is a significant improvement (>102), which is assigned to the large active surface area and high fraction of surface atoms, increasing the amount of greater analyte adsorption during binding. Therefore, water-dispersible MIP-Co-Ni@MOF nanosheets were successfully applied for trace-level determination of FAV in biological and water samples. Our findings seem to provide useful guidance in the molecularly imprinted polymer design of MOF-based materials to help establish quantitative rules in designing MOF-based sensors for point of care (POC) systems.

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“…In addition, COVID-19 can affect numerous important organs, including the heart, blood vessels, kidneys, abdomen, especially when the patients are not of a vulnerable age. Tremendous efforts have been exerted to slow the COVID-19 spread and to protect all individuals, especially those with chronic diseases [4][5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic methods for COVID-19 detection and early diagnosis

Bedair

Okasha

Mansour

2022

Virol J

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The coronavirus pandemic is a worldwide hazard that poses a threat to millions of individuals throughout the world. This pandemic is caused by the severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2), which was initially identified in Wuhan, China's Hubei provincial capital, and has since spread throughout the world. According to the World Health Organization's Weekly Epidemiological Update, there were more than 250 million documented cases of coronavirus infections globally, with five million fatalities. Early detection of coronavirus does not only reduce the spread of the virus, but it also increases the chance of curing the infection. Spectroscopic techniques have been widely used in the early detection and diagnosis of COVID-19 using Raman, Infrared, mass spectrometry and fluorescence spectroscopy. In this review, the reported spectroscopic methods for COVID-19 detection were discussed with emphasis on the practical aspects, limitations and applications.

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Menthol-assisted homogenous liquid-liquid microextraction for HPLC/UV determination of favipiravir as an antiviral for COVID-19 in human plasma

Cited by 37 publications

References 34 publications

The anti-COVID-19 drug Favipiravir: Degradation, Method development, Validation, NMR/LC–MS characterization, and In-vitro safety evaluation

The anti-COVID-19 drug Favipiravir: Degradation, Method development, Validation, NMR/LC–MS characterization, and In-vitro safety evaluation

Electrochemical Sensing of Favipiravir with an Innovative Water-Dispersible Molecularly Imprinted Polymer Based on the Bimetallic Metal-Organic Framework: Comparison of Morphological Effects

Spectroscopic methods for COVID-19 detection and early diagnosis

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