Mercury and human genotoxicity: Critical considerations and possible molecular mechanisms

Crespo-López, María Elena; Macêdo, Gisele L.; Pereira, Susana Isabel; Arrifano, Gabriela de Paula; Picanço-Diniz, Domingos Luiz Wanderley; Nascimento, José Luíz Martins do; Herculano, Anderson Manoel

doi:10.1016/j.phrs.2009.02.011

Cited by 248 publications

(174 citation statements)

References 58 publications

Supporting

Mentioning

153

Contrasting

Unclassified

Order By: Relevance

“…Mechanistically the mercury species target especially microtubules because of the thiol-groups present in tubulin. Depolymerisation of microtubules by mercury species has been shown to disturb numerous cellular processes, including cell survival, proliferation, migration and differentiation (Johansson et al, 2007;Crespo-Lopez et al, 2009 Limke et al, 2004). Mercury exposure has been shown in many cell types, including neuronal cells, to increase cellular Ca 2+ levels, which in turn leads to activation of degradative enzymes, disruption of mitochondrial function and an increase in RONS-induced damage with subsequent cell death.…”

Section: Mechanisms Of Neurotoxicity and Neurodevelopmental Toxicitymentioning

confidence: 99%

Scientific Opinion on the risk for public health related to the presence of mercury and methylmercury in food

2012

EFS2

465

160

View full text Add to dashboard Cite

EFSA was asked by the European Commission to consider new developments regarding inorganic mercury and methylmercury toxicity and evaluate whether the Joint FAO/WHO Expert Committee on Food Additives (JECFA) provisional tolerable weekly intakes for methylmercury of 1.6 µg/kg body weight (b.w.) and of 4 µg/kg b.w. for inorganic mercury were still appropriate. In line with JECFA, the CONTAM Panel established a tolerable weekly intake (TWI) for inorganic mercury of 4 µg/kg b.w., expressed as mercury. For methylmercury, new developments in epidemiological studies from the Seychelles Child Developmental Study Nutrition Cohort have indicated that n-3 long-chain polyunsaturated fatty acids in fish may counteract negative effects from methylmercury exposure. Together with the information that beneficial nutrients in fish may have confounded previous adverse outcomes in child cohort studies from the Faroe Islands, the Panel established a TWI for methylmercury of 1.3 µg/kg b.w., expressed as mercury. The mean dietary exposure across age groups does not exceed the TWI for methylmercury, with the exception of toddlers and other children in some surveys. The 95 th percentile dietary exposure is close to or above the TWI for all age groups. High fish consumers, which might include pregnant women, may exceed the TWI by up to approximately six-fold. Unborn children constitute the most vulnerable group. Biomonitoring data from blood and hair indicate that methylmercury exposure is generally below the TWI in Europe, but higher levels are also observed. Exposure to methylmercury above the TWI is of concern. If measures to reduce methylmercury exposure are considered, the potential beneficial effects of fish consumption should also be taken into account. Dietary inorganic mercury exposure in Europe does not exceed the TWI, but inhalation exposure of elemental mercury from dental amalgam is likely to increase the internal inorganic mercury exposure; thus the TWI might be exceeded. This opinion focuses only on the risks related to dietary inorganic mercury and methylmercury exposure and does not assess the nutritional benefits linked to certain foods (e.g. fish and other seafood).A call for annual collection of chemical contaminant occurrence data in food and feed, including mercury, was issued by EFSA in December 2010. In response, EFSA received 59 820 results on mercury in food from 20 European countries, mainly covering the period from 2004 to 2011. A total number of 59 650 results were described with sufficient detail to be used in the statistical analysis of the respective food groups; 98.2 % of the samples were for total mercury, 1.8 % for methylmercury and three samples for inorganic mercury.All the 20 food groups available at the first level of FoodEx were covered in the current data collection. The food groups 'Fish and other seafood' and 'Meat and meat products' dominated the food product coverage with 36.8 % and 17.6 % respectively. These were followed by 'Grain and grain-based products' at 7.8 % and 'Vegetables an...

show abstract

Section: Mechanisms Of Neurotoxicity and Neurodevelopmental Toxicitymentioning

confidence: 99%

Scientific Opinion on the risk for public health related to the presence of mercury and methylmercury in food

2012

EFS2

465

160

View full text Add to dashboard Cite

show abstract

“…On the other hand, findings of this study documented fact that the adenocarcinoma of human prostate accumulates high levels of many trace elements including Al, B, Be, Bi, Cr, Fe, Hg, Ni, Sn, Th, and Zr. Genotoxicity and carcinogenicity of these elements are well known (Anghileri et al, 2000;Gordon & Bowser, 2003;Crespo-López et al, 2009;Bian et al, 2011;Gonzalez-Vasconcellos et al, 2011;Müezzinoğlu et al, 2011;Sappino et al, 2012;Helmig et al, 2013;Adámik et al, 2015;Farasani & Darbre, 2015). On the other hand, compared to human body soft tissues, the normal prostate of young adults has higher levels of many trace elements, including Zn (V. Zaichick & S. Zaichick, 2013, 2013a, 2013b, 2013c, 2014d.…”

Section: Resultsmentioning

confidence: 99%

Prostatic Tissue Levels of 43 Trace Elements in Patients with Prostate Adenocarcinoma

Zaichick

2016

CCO

View full text Add to dashboard Cite

Adenocarcinoma of prostate gland is an internationally important health problem in men, particularly in developed countries. The aim of this exploratory study was to evaluate whether significant changes in the prostatic tissue levels of trace elements exist in the malignantly transformed prostate. Prostatic tissue levels of 43 trace elements were prospectively evaluated in 36 patients with prostate adenocarcinoma and 37 healthy males. Measurements were performed using a combination of non-destructive and destructive methods: instrumental neutron activation analysis and inductively coupled plasma mass spectrometry, respectively. Tissue samples were divided into two portions. One was used for morphological study while the other was intended for trace element analysis. The reliability of difference in the results between normal and cancerous prostate tissues was evaluated by Student's t-test. It was found that the contents of Ag, Al, Au, B, Be, Br, Ce, Cr, Dy, Er, Fe, Gd, Hg, Li, Mn, Nd, Ni, Pr, Sb, Sn, Th, Tl, Y, and Zr were significantly higher while those of Cd, Co, Rb, Sc, Se, and Zn were significantly lower in cancerous tissues than in normal tissues. Moreover, it was shown that malignant transformation significantly changed the relationships of trace elements in prostate. Thus, in adenocarcinoma transformed prostate tissue the trace element metabolism is significantly disturbed.

show abstract

“…It is well known that several Hg compounds induce neurotoxicity, and MeHg is more toxic than inorganic mercury (Fretham et al, 2012). A commonly studied mechanism of Hg toxicity is the generation of reactive oxygen species and resulting macromolecular damage and alteration of signal transduction pathways (Crespo-López et al, 2009). A comparison of genotoxicity between inorganic and organic mercury compounds revealed that MeHg induced DNA damage at significantly lower concentrations than inorganic mercury (Ogura et al, 1996).…”

Section: Influence Of Metal Compounds On Fpg Activitymentioning

confidence: 99%

“…Thus, glycosylases have become important biomarkers and potential therapeutic targets for related diseases (Helleday et al, 2008). Some metal ions have been shown to be carcinogenic to humans and/or experimental animals, but the mechanisms involved in tumor formation have been not resolved yet (Crespo-López et al, 2009;Hartwig, 2013 in metal induced geno-toxicity is the formation of oxidatively damaged DNA products such as 8-oxodGuo and DNA strand breaks (Lloyd and Phillips, 1999). Another mechanism, which has attracted more attention recently, is the inactivation of DNA repair system by the metal ions (Hartwig, 1998;Hartwig et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

An electrochemiluminescence biosensor for 8-oxo-7,8-dihydro-2′-deoxyguanosine quantification and DNA repair enzyme activity analysis using a novel bifunctional probe

Yang

Zhang

et al. 2015

Biosensors and Bioelectronics

View full text Add to dashboard Cite

a b s t r a c tA new electrochemiluminescence (ECL) sensor was developed for 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo) quantification and Escherichia coli formamidopyrimidine-DNA glycosylase (FPG) activity assay. The sensor employed a novel spermine conjugated ruthenium tris-(bipyridine) derivative (spermine-Ru) which binds specifically with 8-oxodGuo through a one-step reaction and also acts as an ECL signal reporter. In the sensor, an 8-oxodGuo-containing ds-DNA film was first immobilized on a gold electrode by self-assembly. The DNA film was then incubated with spermine-Ru under oxidative condition for 8-oxodGuo labeling. The ECL intensity was found to correlate with the amount of 8-oxodGuo on the surface and the detection limit was estimated to be about 1 lesion in 500 DNA bases. Addition of FPG resulted in some loss of the signal due to the excision of 8-oxodGuo by the enzyme. An inverse relationship between ECL intensity and FPG concentration was observed in a range from 0 to 4.0 U/mL, demonstrating that this sensor could be used for FPG activity assay. A number of metal ions were screened by the sensor for their inhibition effect on FPG activity. Among them, Hg 2 þ and methyl Hg(II)shown very potent inhibition, with IC 50 values of 4.04 mM and 4.34 nM respectively. The result may suggest that interference on the DNA repair system could be another mechanism for the high toxicity of MeHg.

show abstract

Mercury and human genotoxicity: Critical considerations and possible molecular mechanisms

Cited by 248 publications

References 58 publications

Scientific Opinion on the risk for public health related to the presence of mercury and methylmercury in food

Scientific Opinion on the risk for public health related to the presence of mercury and methylmercury in food

Prostatic Tissue Levels of 43 Trace Elements in Patients with Prostate Adenocarcinoma

An electrochemiluminescence biosensor for 8-oxo-7,8-dihydro-2′-deoxyguanosine quantification and DNA repair enzyme activity analysis using a novel bifunctional probe

Contact Info

Product

Resources

About