Objectives:The study aimed at assessing the effect of low-level exposure to mercury (Hg) vapor from amalgam fi llings among dental surgery staff in the city of Łódź, Poland. Materials and Methods: The study group was composed of 51 workers (mean age, 39.25±11.05 years) employed in dental surgeries; the control group comprised 16 white-collar workers (mean age, 40.05±10.57 years) of the Nofer Institute of Occupational Medicine, Łódź, who had no or a few amalgam dental fi llings. Total urine mercury (Hg-U) concentration was determined with cold vapor atomic absorption spectrometry (CV-AAS) based on tin (II) chloride mercury reduction after overnight digestion of urine sample with potassium permanganate in sulfuric acid solution. The reliability of mercury determinations was verifi ed by measuring Hg concentration in the reference material and via participation in the external quality assessment scheme. Results: No statistically signifi cant differences were found in geometric mean of urine Hg concentrations between the study and control groups (GM±SD, 0.44±0.440 and 0.5±0.270 μg/g creatinine, respectively, p = 0.242). Among different factors affecting Hg exposure in dental surgeries, only the duration of dental practice showed a statistically signifi cant infl uence on total Hg-U (r = 0.3000; p = 0.024). Having divided the subjects into two groups, with and without amalgam fi llings, we noted a statistically signifi cant difference in urine Hg concentrations (0.60 ± 0.720, n = 38; 0.36 ± 0.650, n = 29; p = 0.004) between these two groups. Conclusions: The results of biological monitoring of dental surgery staff did not reveal current exposure to Hg vapors from amalgam fi llings. However, these dental workers may have been the subject to such an exposure in the past. The only statistically signifi cant correlation was that between total Hg-U concentrations and the duration of dental practice.