Meropenem, levofloxacin and linezolid in human plasma of critical care patients: A fast semi-automated micro-extraction by packed sorbent UHPLC-PDA method for their simultaneous determination

Ferrone, Vincenzo; Cotellese, Roberto; Marco, Lorenzo Di; Bacchi, Simona; Carlucci, Maura; Cichella, Annadomenica; Raimondi, Paolo; Carlucci, Giuseppe

doi:10.1016/j.jpba.2017.03.035

Cited by 24 publications

(6 citation statements)

References 27 publications

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“…Methodology to quantify drugs in biological fluids is important to support pre-clinical and clinical studies. Chromatographic methods have been reported for meropenem in plasma [10][11][12][13][14][15][16][17][18][19][20][21][22][23] and serum [24][25][26][27] using UV [12,16,26], diode array [15,20,28,29], and mass spectrometry [10, 11, 13, 14, 17-19, 21-25, 27, 30] detection. To the best of our knowledge, there are currently no analytical methods reporting the quantification of the combination of meropenem-vaborbactam in biological fluids and no methods for the quantification of newly developed β-lactamase inhibitor, vaborbactam.…”

Section: Introductionmentioning

confidence: 99%

A validated LC-MSMS method for the simultaneous quantification of meropenem and vaborbactam in human plasma and renal replacement therapy effluent and its application to a pharmacokinetic study

et al. 2019

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A simple method for the simultaneous quantification of meropenem and the recently approved β-lactamase inhibitor, vaborbactam, in human plasma and renal replacement therapy effluent (RRTE) was developed and validated. This antibiotic combination protects a primary β-lactam, meropenem, with a new β-lactamase inhibitor, and expands the limited options for treatment of multidrug-resistant Gram-negative infections. Meropenem, vaborbactam, and the internal standards [ 2 H 6 ]meropenem and sulbactam in plasma and RRTE were processed using acetonitrile followed by a chromatographic separation on a Poroshell HPH-C18 column with a gradient elution of the mobile phases and monitored using mass spectrometry detection. The calibration range was 0.05 to 100 μg mL −1 for both meropenem and vaborbactam. The intra-day and inter-day precision and accuracy were less than 15% for both meropenem and vaborbactam and the recovery from plasma was 96% for both meropenem and vaborbactam and the recovery from RRTE was 93% and 103% for meropenem and vaborbactam, respectively. This methodology was successfully applied to an ex vivo characterisation study of the effects of renal replacement therapy modalities on the pharmacokinetics of meropenem and vaborbactam (Antimicrob Agents Chemother 62(10), 2018).

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Section: Introductionmentioning

confidence: 99%

A validated LC-MSMS method for the simultaneous quantification of meropenem and vaborbactam in human plasma and renal replacement therapy effluent and its application to a pharmacokinetic study

et al. 2019

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“…A numerous HPLC methods with UV detection were developed for quantification of β-lactam antibiotics, both for the quantification of individual representatives of beta-lactam antibiotics (12,(98)(99)(100)(101)(102)(103)(104)(105)(106)(107)(108)(109)(110)(111)(112)(113)(114) and for simultaneous quantification of several antibiotics from this group (2,8,57,65,76,77,(115)(116)(117)(118)(119)(120)(121)(122)(123)(124)(125)(126)(127). More recent studies have used ultra high-performance liquid chromatography (UHPLC) as method for the determination of antibiotics, with UV-diode array detector (DAD) or photo diode array (PDA) (121,(128)(129)(130)(131)(132)(133).…”

Section: Methods Of Quantifying β-Lactam Antibiotics In Biological Sa...mentioning

confidence: 99%

Methods for Determination of Meropenem Concentration in Biological Samples

Rančić

2022

Serbian Journal of Experimental and Clinical Research

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Measuring the concentration of antibiotics in biological samples allow implementation of therapeutic monitoring of these drugs and contribute to the adjustment of the dosing regimen in patients. This increases the effectiveness of antimicrobial therapy, reduces the toxicity of these drugs and prevents the development of bacterial resistance. This review article summarizes current knowledge on methods for determining concentration of meropenem, an antibiotic drug from the group of carbapenems, in different biological samples. It provides a brief discussion of the chemical structure, physicochemical and pharmacokinetic properties of meropenem, different sample preparation techniques, use of apparatus and equipment, knowledge of the advantages and limitations of available methods, as well as directions in which new methods should be developed. This review should facilitate clinical laboratories to select and apply one of the established methods for measuring of meropenem, as well as to provide them with the necessary knowledge to develop new methods for quantification of meropenem in biological samples according to their needs.

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“…It has activity to both Gram‐positive and Gram negative bacteria and anaerobes. LEV containing pharmaceuticals are commonly used for the treatment of respiratory tract infections, skin infections and genitourinary tract infections . Quantitative determination of LEV has been studied by several analytical techniques such as spectrofluorimetry , nuclear magnetic resonance spectrometry , high‐performance liquid chromatography coupled with MS/MS , high performance liquid chromatography with photodiode array detector , UV spectrophotometric method and electrochemical technique .…”

Section: Introductionmentioning

confidence: 99%

Poly(Taurine‐Glutathione)/Carbon Nanotube Modified Glassy Carbon Electrode as a New Levofloxacin Sensor

Koçak

2019

Electroanalysis

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A new highly sensitive and selective electrochemical levofloxacin sensor based on co‐polymer‐carbon nanotube composite electrode was developed. Taurine and Glutathione were electrochemically co‐polymerized on multiwalled carbon nanotubes modified glassy carbon electrode (Poly(TAU‐GSH)/CNT/GCE) and used as a levofloxacin sensor in pH 6 phosphate buffer solution. The new composite electrode surfaces were characterized by scanning electron microscopy, atomic force microscopy and electrochemical impedance spectroscopy. Under the optimized conditions, two linear segments were obtained for increasing LEV concentrations between 20 nmol L−1‐1 μmol L−1 and 1.5 μmol L−1‐55 μmol L−1 LEV with a detection limit of 9 nmol L−1 using amperometry. Poly(TAU‐GSH)/CNT/GCE exhibited high sensitivity, selectivity with good stability. The new sensor was employed for real samples of LEV tablets and urine. Promising results were obtained with good accuracy which were also in accordance with LC‐MS/MS analysis.

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Meropenem, levofloxacin and linezolid in human plasma of critical care patients: A fast semi-automated micro-extraction by packed sorbent UHPLC-PDA method for their simultaneous determination

Cited by 24 publications

References 27 publications

A validated LC-MSMS method for the simultaneous quantification of meropenem and vaborbactam in human plasma and renal replacement therapy effluent and its application to a pharmacokinetic study

A validated LC-MSMS method for the simultaneous quantification of meropenem and vaborbactam in human plasma and renal replacement therapy effluent and its application to a pharmacokinetic study

Methods for Determination of Meropenem Concentration in Biological Samples

Poly(Taurine‐Glutathione)/Carbon Nanotube Modified Glassy Carbon Electrode as a New Levofloxacin Sensor

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