MERS-CoV virus-like particles produced in insect cells induce specific humoural and cellular imminity in rhesus macaques

Wang, Chong; Zheng, Xiaodong; Gai, Weiwei; Zhao, Yongkun; Wang, Hualei; Wang, Haijun; Feng, Na; Chi, Hang; Qiu, Boning; Li, Nan; Wang, Tiecheng; Gao, Yuwei; Wang, Cuiling; Xia, Xianzhu

doi:10.18632/oncotarget.8475

Cited by 140 publications

(169 citation statements)

References 29 publications

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“…2.8. RBD-specific antibody measurement in the sera of mice RBD-specific IgG antibodies from the sera of immunized and control mice were measured by indirect ELISA as described previously (Wang et al, 2016). Briefly, 96-well microtiter plates (Corning Costar, USA) were pre-coated with 100 mL of purified RBD antigen at a final concentration of 1 mg/mL and incubated overnight.…”

Section: Immunization Studiesmentioning

confidence: 99%

Novel chimeric virus-like particles vaccine displaying MERS-CoV receptor-binding domain induce specific humoral and cellular immune response in mice

et al. 2017

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Middle East respiratory syndrome coronavirus (MERS-CoV) has continued spreading since its emergence in 2012 with a mortality rate of 35.6%, and is a potential pandemic threat. Prophylactics and therapies are urgently needed to address this public health problem. We report here the efficacy of a vaccine consisting of chimeric virus-like particles (VLP) expressing the receptor binding domain (RBD) of MERS-CoV. In this study, a fusion of the canine parvovirus (CPV) VP2 structural protein gene with the RBD of MERS-CoV can self-assemble into chimeric, spherical VLP (sVLP). sVLP retained certain parvovirus characteristics, such as the ability to agglutinate pig erythrocytes, and structural morphology similar to CPV virions. Immunization with sVLP induced RBD-specific humoral and cellular immune responses in mice. sVLP-specific antisera from these animals were able to prevent pseudotyped MERS-CoV entry into susceptible cells, with neutralizing antibody titers reaching 1: 320. IFN-γ, IL-4 and IL-2 secreting cells induced by the RBD were detected in the splenocytes of vaccinated mice by ELISpot. Furthermore, mice inoculated with sVLP or an adjuvanted sVLP vaccine elicited T-helper 1 (Th1) and T-helper 2 (Th2) cell-mediated immunity. Our study demonstrates that sVLP displaying the RBD of MERS-CoV are promising prophylactic candidates against MERS-CoV in a potential outbreak situation.

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Section: Immunization Studiesmentioning

confidence: 99%

Novel chimeric virus-like particles vaccine displaying MERS-CoV receptor-binding domain induce specific humoral and cellular immune response in mice

et al. 2017

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“…In comparison to typical virus-like particle preparations, the present strategy offers practical advantages owing to its simple and facile process. Amidst the growing health threats of coronavirus infections as well as the ongoing economic impact of IBV infections, virus-like particles are garnering increasing scientific interest as vaccine candidates owing to their improved efficacy in comparison to subunit antigens [49,50]. In the present study, vaccination with the sVLPs resulted in enhanced humoral and cellular immune responses, improving protection against an avian model of coronavirus infection as compared to free protein antigens and a commercial WIV vaccine.…”

Section: Resultsmentioning

confidence: 69%

Synthetic virus-like particles prepared via protein corona formation enable effective vaccination in an avian model of coronavirus infection

et al. 2016

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The ongoing battle against current and rising viral infectious threats has prompted increasing effort in the development of vaccine technology. A major thrust in vaccine research focuses on developing formulations with virus-like features towards enhancing antigen presentation and immune processing. Herein, a facile approach to formulate synthetic virus-like particles (sVLPs) is demonstrated by exploiting the phenomenon of protein corona formation induced by the high-energy surfaces of synthetic nanoparticles. Using an avian coronavirus spike protein as a model antigen, sVLPs were prepared by incubating 100 nm gold nanoparticles in a solution containing an optimized concentration of viral proteins. Following removal of free proteins, antigen-laden particles were recovered and showed morphological semblance to natural viral particles under nanoparticle tracking analysis and transmission electron microscopy. As compared to inoculation with free proteins, vaccination with the sVLPs showed enhanced lymphatic antigen delivery, stronger antibody titers, increased splenic T-cell response, and reduced infection-associated symptoms in an avian model of coronavirus infection. Comparison to a commercial whole inactivated virus vaccine also showed evidence of superior antiviral protection by the sVLPs. The study demonstrates a simple yet robust method in bridging viral antigens with synthetic nanoparticles for improved vaccine application; it has practical implications in the management of human viral infections as well as in animal agriculture.

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“…WNV-VLP were produced and purified as previously described [22], and 4–6-year-old healthy horses were intramuscularly immunized with WNV-VLP with Freund’s adjuvant and boosted every 2 weeks for 4 cycles. Serial serum samples were collected.…”

Section: Resultsmentioning

confidence: 99%

Equine Immunoglobulin and Equine Neutralizing F(ab′)2 Protect Mice from West Nile Virus Infection

Wang

Zhao

Wang

et al. 2016

Viruses

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West Nile virus (WNV) is prevalent in Africa, Europe, the Middle East, West Asia, and North America, and causes epidemic encephalitis. To date, no effective therapy for WNV infection has been developed; therefore, there is urgent need to find an efficient method to prevent WNV disease. In this study, we prepared and evaluated the protective efficacy of immune serum IgG and pepsin-digested F(ab′)2 fragments from horses immunized with the WNV virus-like particles (VLP) expressing the WNV M and E proteins. Immune equine F(ab′)2 fragments and immune horse sera efficiently neutralized WNV infection in tissue culture. The passive transfer of equine immune antibodies significantly accelerated the virus clearance in the spleens and brains of WNV infected mice, and reduced mortality. Thus, equine immunoglobulin or equine neutralizing F(ab′)2 passive immunotherapy is a potential strategy for the prophylactic or therapeutic treatment of patients infected with WNV.

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MERS-CoV virus-like particles produced in insect cells induce specific humoural and cellular imminity in rhesus macaques

Cited by 140 publications

References 29 publications

Novel chimeric virus-like particles vaccine displaying MERS-CoV receptor-binding domain induce specific humoral and cellular immune response in mice

Novel chimeric virus-like particles vaccine displaying MERS-CoV receptor-binding domain induce specific humoral and cellular immune response in mice

Synthetic virus-like particles prepared via protein corona formation enable effective vaccination in an avian model of coronavirus infection

Equine Immunoglobulin and Equine Neutralizing F(ab′)2 Protect Mice from West Nile Virus Infection

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