Mesenchymal stem cell‐conditioned medium accelerates wound healing with fewer scars

Li, Meirong; Luan, Fuxin; Zhao, Yali; Hao, Haojie; Liu, Jiejie; Dong, Liang; Fu, Xiaobing; Han, Weidong

doi:10.1111/iwj.12551

Cited by 85 publications

(93 citation statements)

References 42 publications

(54 reference statements)

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“…They express such antigens as CD73, CD90 and Cd105 and should not express CD14, D19, CD34, CD45, CD11b, CD79a and HLA-DR surface molecules. 3,6,9,[139][140][141][142] Bone marrow, the umbilical cord, adipose tissue, placenta and cord blood all provide sources of MSCs ( Figure 6) containing the therapeutic potential for the treatment of wound healing disorders. 143 With respect to normal cutaneous wound healing, MSCs are mobilized from their host sources to the injury site where they support skin repair despite hypoxia and a lack of nutrients.…”

Section: Mscs and Their Putative Mechanism Of Action In Wound Repairmentioning

confidence: 99%

<p>The effects of graphene and mesenchymal stem cells in cutaneous wound healing and their putative action mechanism</p>

Lasocka¹,

Jastrzębska²,

Szulc-Dąbrowska³

et al. 2019

IJN

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This study provides a review of the therapeutic potential of graphene dressing scaffolds and mesenchymal stem cells (MSCs) and their synergistic effects with respect to cutaneous wound healing. This study also considers their putative action mechanism based on the antibacterial, immunomodulating, angiogenic, matrix remodeling effects of materials belonging to the graphene family and MSCs during the wound healing process. In addition, this study discusses the cytocompatibility of graphene, its uses as a platform for skin substitutes, the properties it possesses with respect to providing protection against microbial invasion as well as strategies aimed at minimizing the chance of the occurrence of sepsis. MSCs are capable of secreting several factors that exert a therapeutic impact on reparative processes and tissue regeneration. In light of experiments conducted to date, graphene combined with MSCs appears to have the potential to enhance both the wound healing process and infection control at the injury site.

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Section: Mscs and Their Putative Mechanism Of Action In Wound Repairmentioning

confidence: 99%

<p>The effects of graphene and mesenchymal stem cells in cutaneous wound healing and their putative action mechanism</p>

Lasocka¹,

Jastrzębska²,

Szulc-Dąbrowska³

et al. 2019

IJN

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show abstract

“…Wounds treated with the UC-MSC conditioned media also healed faster with decreased collagen accumulation. (Li, et al, 2015) Additionally, in vitro studies have shown that human amniotic fluid-derived MSC conditioned media has the potential to inhibit the pro-fibrotic actions of TGF-β1 and even reverse the myofibroblast phenotype to a fibroblast-like state. Conditioned media from ASCs produced similar results, but to a lesser extent.…”

Section: Recent Advances In Scarless Wound Healing Researchmentioning

confidence: 99%

Scarless wound healing: finding the right cells and signals

et al. 2016

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From the moment we are born, every injury to the skin has the potential to form a scar, many of which can impair form and/or function. As such, scar management constitutes a billion-dollar industry. Yet effectively promoting scarless wound healing remains an elusive goal. The complex interactions of wound healing contribute to our inability to recapitulate scarless wound repair as it occurs in nature, such as in fetal skin and oral mucosa. However, many new advances have occurred over recent years, some of which have translated scientific findings from bench to bedside. In vivo lineage tracing has helped establish a variety of novel cellular culprits that may act as key drivers of the fibrotic response. These newly characterized cell populations present further targets for therapeutic intervention, some of which have already demonstrated promising results in animal models. Here, we discuss several recent studies that identify exciting approaches to diminishing scar formation (Fig. 1). Particular attention will also be paid to the canonical Wnt/β-catenin signaling pathway, which plays an important role in both embryogenesis and tissue repair. New insights into the differential effects of Wnt signaling on heterogeneous fibroblast and keratinocyte populations within the skin further demonstrate methods by which wound healing can be redirected to a more fetal, scarless phenotype.

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“…Tang et al also reported that DPSCs had inhibitory effects on the proliferation of PBMCs [16]. Some studies reported that the conditioned-medium mesenchymal stem cells had anti-inflammatory effects [17,18]. Yamaguchi and collogues showed that DPSCs-CM could reduce cardiac injury [19].…”

Section: Discussionmentioning

confidence: 98%

The Effects of Dental Pulp Stem Cell Conditioned Media on the Proliferation of Peripheral Blood Mononuclear Cells

et al. 2019

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Background: Dental Pulp Stem Cells (DPSCs) are multipotent mesenchymal stem cells. DPSCs can renew themselves and differentiate into various cell types such as adipocytes, osteocytes, neurons, etc. DPSCs possess immunomodulatory properties and can inhibit Peripheral Blood Mononuclear Cell (PBMC) proliferation. Recent studies showed that conditioned-medium mesenchymal stem cells also had immunosuppressive activity. The ability of DPSC conditioned medium to suppress proliferation of allogeneic PBMC determined using BrdU (5-bromo-2'-deoxyuridine) proliferation assay. Materials and Methods:Dental pulp stem cells were extracted from a wisdom tooth. These cells are characterized for differentiation potential to adipogenic and osteogenic lineage and expression of mesenchymal stem cells markers, including CD105, CD73, CD90, CD14, CD-34, and CD45. The characterized DPSCs were cultured, and the Conditioned Medium (CM) got isolated. Stimulated and non-stimulated PBMCs from the allogeneic donor were cultured with DPSC-CM for 24, 48, and 72 hours. The proliferation of PBMCs was measured with BrdU assay. Results:The BrdU test results showed that DPSC-CM reduced allogeneic PBMC proliferation at different time points. DPSC-CM could inhibit stimulated and non-stimulated PBMC in 48 and 72 hours after incubation. Conclusion:This study demonstrated that DPSC-CM had an immunomodulatory effect on the proliferation of allogeneic cells.

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Mesenchymal stem cell‐conditioned medium accelerates wound healing with fewer scars

Cited by 85 publications

References 42 publications

<p>The effects of graphene and mesenchymal stem cells in cutaneous wound healing and their putative action mechanism</p>

<p>The effects of graphene and mesenchymal stem cells in cutaneous wound healing and their putative action mechanism</p>

Scarless wound healing: finding the right cells and signals

The Effects of Dental Pulp Stem Cell Conditioned Media on the Proliferation of Peripheral Blood Mononuclear Cells

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