Mesenchymal stem cell markers in periodontal tissues and periapical lesions

Carvalho, Larissa Abbehusen Couto De; Santos, Simone Leal Tosta dos; Sacramento, Lorena Vieira; Júnior, Vildeman Rodrigues de Almeida; Xavier, Flávia Caló Aquino; Santos, Jean Nunes dos; Henriques, Águida Cristina Gomes

doi:10.1016/j.acthis.2020.151636

Cited by 12 publications

(8 citation statements)

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“…According to these literature data, our results show that DPSCs, when exposed to hypoxia, change their morphology: growth potential decreased after 16 days of treatment at 1% O 2 . Moreover, when exposed to hypoxia 1%, DPSCs, at both times considered, were induced to differentiate vs. a neuronal phenotype, showing a switch, strongly relevant after 16 days, of mesenchymal stem cells markers CD44, CD90, CD105, and CD73, commonly expressed by DPSCs vs. neuronal markers nestin, β3-Tubulin, NFH, and GAP 43 [59]. The commitment vs. a neuronal phenotype was also confirmed by the mRNA expression profile of the DPSCs exposed (5H, 16H), or not (5N, 16N), to hypoxia.…”

Section: Discussionmentioning

confidence: 98%

“…To evaluate a possible role of hypoxic conditioning in the induction of neuronal differentiation, DPSCs were exposed for 5 and 16 days to hypoxia at 1% O 2 (5H, 16H) or maintained in normoxia at 21% O 2 (5N, 16N). Figure 2A shows that all mesenchymal stem cell markers (CD44, CD90, CD105, STRO1, and CD73) commonly expressed by DPSCs [59] decreased in the cells exposed to hypoxia after 5 days. These markers decreased from 69.5, 70.8, 34.2, 68.1 and 74.4% to 76.4, 75.1, 63.9, 73.4 and 84.22% in the normoxic cells, respectively.…”

Section: Comparative Characterization Of Dpscs' Stem and Neuronal Mar...mentioning

confidence: 99%

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Hypoxia Induces DPSC Differentiation versus a Neurogenic Phenotype by the Paracrine Mechanism

et al. 2022

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As previously described by several authors, dental pulp stem cells (DPSCs), when adequately stimulated, may acquire a neuronal-like phenotype acting as a favorable source of stem cells in the generation of nerves. Besides, it is known that hypoxia conditioning is capable of stimulating cell differentiation as well as survival and self-renewal, and that multiple growth factors, including Epidermal Growth factor (EGF) and basic fibroblast growth factor (bFGF), are often involved in the induction of the neuronal differentiation of progenitor cells. In this work, we investigated the role of hypoxia in the commitment of DPSCs into a neuronal phenotype. These cells were conditioned with hypoxia (O2 1%) for 5 and 16 days; subsequently, we analyzed the proliferation rate and morphology, and tested the cells for neural and stem markers. Moreover, we verified the possible autocrine/paracrine role of DPSCs in the induction of neural differentiation by comparing the secretome profile of the hypoxic and normoxic conditioned media (CM). Our results showed that the hypoxia-mediated DPSC differentiation was time dependent. Moreover, conditioned media (CM derived from DPSCs stimulated by hypoxia were able, in turn, to induce the neural differentiation of SH-SY5Y neuroblastoma cells and undifferentiated DPSCs. In conclusion, under the herein-mentioned conditions, hypoxia seems to favor the differentiation of DPSCs into neuron-like cells. In this way, we confirm the potential clinical utility of differentiated neuronal DPSCs, and we also suggest the even greater potential of CM-derived-hypoxic DPSCs that could more readily be used in regenerative therapies.

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Section: Discussionmentioning

confidence: 98%

Section: Comparative Characterization Of Dpscs' Stem and Neuronal Mar...mentioning

confidence: 99%

Hypoxia Induces DPSC Differentiation versus a Neurogenic Phenotype by the Paracrine Mechanism

et al. 2022

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“…CD90 influences cell-cell and cell-matrix interactions, cell adhesion and migration, cytoskeleton organization, and tissue regeneration [40]. Reduced expression of CD90 was not associated with modifications of the morphology, proliferation rate, or immunosuppressive potential of MSCs, but augmented the differentiation potential of MSCs toward osteogenic and adipogenic lineages [41].…”

Section: Discussionmentioning

confidence: 99%

Oral Mesenchymal Stromal Cells in Systemic Sclerosis: Characterization and Response to a Hyaluronic-Acid-Based Biomaterial

et al. 2021

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Introduction. As oral mesenchymal stromal cells (MSCs) have not, to date, been isolated from systemic sclerosis (SSc) patients, the aim of this in vitro experiment was to characterize gingival MSCs (SScgMSCs) and granulation tissue MSCs (SScgtMSCs) from SSc and to evaluate their functionality in comparison to healthy MSCs (hMSCs), in normal or hyaluronic acid (HA) culture media. Materials and Methods. Isolated cells were described by immunophenotyping of surface antigen make-up and by trilineage mesenchymal differentiation capacity. Colony-Forming Unit-Fibroblast (CFU-F) test and migration potential evaluated MSC functionality. Results. All types of MSCs displayed positivity for the following surface markers: CD29, CD73, CD90, CD105, CD44, and CD79a. These cells did not express CD34, CD45, HL-DR, and CD14. Isolated MSCs differentiated into osteoblasts, adipocytes, and chondroblasts. The frequency of CFU-F for SScgtMSCs was significantly lower than that of hMSCs (p = 0.05) and SScgMSCs (p = 0.004) in normal medium, and also markedly lower than that of SScgMSCs (p = 0.09) in HA medium. Following HA exposure, both SScgMSCs and SScgtMSCs migrated significantly less (p = 0.033 and p = 0.005, respectively) than hMSCs. Conclusions. A reduced functionality of MSCs derived from SSc as compared to hMSCs was observed. HA in culture medium appeared to significantly stimulate the migration potential of hMSCs.

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“…MSCs derived from adult tissues (e.g., bone marrow, adipose tissue, and synovial tissue) present no ethical or legal concerns, can be expanded in vitro, and used in TE strategies [41,42]. PDLSCs show similar characteristics to MSCs, such as fibroblast-like morphology, multilineage differentiation capacity, and expression of MSC-related surface markers [43][44][45]. PDLSCs are present in the PDL and serve as a source for renewable progenitor cells, which differentiate into osteoblasts, cementoblasts, and fibroblasts, responsible for bone, cementum, and PDL formation [14].…”

Section: Periodontal Tissue Engineeringmentioning

confidence: 99%

Recent Advances on Electrospun Nanofibers for Periodontal Regeneration

2023

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Periodontitis is an inflammatory infection caused by bacterial plaque accumulation that affects the periodontal tissues. Current treatments lack bioactive signals to induce tissue repair and coordinated regeneration of the periodontium, thus alternative strategies are needed to improve clinical outcomes. Electrospun nanofibers present high porosity and surface area and are able to mimic the natural extracellular matrix, which modulates cell attachment, migration, proliferation, and differentiation. Recently, several electrospun nanofibrous membranes have been fabricated with antibacterial, anti-inflammatory, and osteogenic properties, showing promising results for periodontal regeneration. Thus, this review aims to provide an overview of the current state of the art of these nanofibrous scaffolds in periodontal regeneration strategies. First, we describe the periodontal tissues and periodontitis, as well as the currently available treatments. Next, periodontal tissue engineering (TE) strategies, as promising alternatives to the current treatments, are addressed. Electrospinning is briefly explained, the characteristics of electrospun nanofibrous scaffolds are highlighted, and a detailed overview of electrospun nanofibers applied to periodontal TE is provided. Finally, current limitations and possible future developments of electrospun nanofibrous scaffolds for periodontitis treatment are also discussed.

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Mesenchymal stem cell markers in periodontal tissues and periapical lesions

Cited by 12 publications

References 123 publications

Hypoxia Induces DPSC Differentiation versus a Neurogenic Phenotype by the Paracrine Mechanism

Hypoxia Induces DPSC Differentiation versus a Neurogenic Phenotype by the Paracrine Mechanism

Oral Mesenchymal Stromal Cells in Systemic Sclerosis: Characterization and Response to a Hyaluronic-Acid-Based Biomaterial

Recent Advances on Electrospun Nanofibers for Periodontal Regeneration

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