Mesenchymal Stem Cells-Derived Exosomes for Wound Regeneration

Goodarzi, Parisa; Larijani, Bagher; Alavi-Moghadam, Sepideh; Tayanloo-Beik, Akram; Mohamadi-Jahani, Fereshteh; Ranjbaran, Negar; Payab, Moloud; Falahzadeh, Khadijeh; Mousavi, M J; Arjmand, Babak

doi:10.1007/5584_2018_251

Cited by 87 publications

(60 citation statements)

References 97 publications

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“…One paracrine mechanism of MSCs involves the secretion of EVs that have been shown to effectively mimic the therapeutic effects of MSCs, participating in tissue repair and regeneration in several preclinical models [33][34][35].…”

Section: Role Of Extracellular Vesiclesmentioning

confidence: 99%

Paracrine Mechanisms of Mesenchymal Stromal Cells in Angiogenesis

Maacha

Sidahmed²,

Jacob

et al. 2020

Stem Cells International

182

131

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The role of the mesenchymal stromal cell- (MSC-) derived secretome is becoming increasingly intriguing from a clinical perspective due to its ability to stimulate endogenous tissue repair processes as well as its effective regulation of the immune system, mimicking the therapeutic effects produced by the MSCs. The secretome is a composite product secreted by MSC in vitro (in conditioned medium) and in vivo (in the extracellular milieu), consisting of a protein soluble fraction (mostly growth factors and cytokines) and a vesicular component, extracellular vesicles (EVs), which transfer proteins, lipids, and genetic material. MSC-derived secretome differs based on the tissue from which the MSCs are isolated and under specific conditions (e.g., preconditioning or priming) suggesting that clinical applications should be tailored by choosing the tissue of origin and a priming regimen to specifically correct a given pathology. MSC-derived secretome mediates beneficial angiogenic effects in a variety of tissue injury-related diseases. This supports the current effort to develop cell-free therapeutic products that bring both clinical benefits (reduced immunogenicity, persistence in vivo, and no genotoxicity associated with long-term cell cultures) and manufacturing advantages (reduced costs, availability of large quantities of off-the-shelf products, and lower regulatory burden). In the present review, we aim to give a comprehensive picture of the numerous components of the secretome produced by MSCs derived from the most common tissue sources for clinical use (e.g., AT, BM, and CB). We focus on the factors involved in the complex regulation of angiogenic processes.

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Section: Role Of Extracellular Vesiclesmentioning

confidence: 99%

Paracrine Mechanisms of Mesenchymal Stromal Cells in Angiogenesis

Maacha

Sidahmed²,

Jacob

et al. 2020

Stem Cells International

182

131

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“…Cell therapy is based on the ability of MSCs to migrate to the sites of pathology. They are able to exert anti-inflammatory and immunomodulatory effects upon allogeneic transplantation, as well as in autoimmune diseases [2][3][4][5]. Obtaining genetically modified MSCs expressing introduced genes significantly expands the possibilities of both cellular and genetic therapy, ensuring the delivery of therapeutic molecules to the sites of damage and inflammation [6,7].…”

Section: Introductionmentioning

confidence: 99%

Genetically Modified Mouse Mesenchymal Stem Cells Expressing Non-Structural Proteins of Hepatitis C Virus Induce Effective Immune Response

et al. 2020

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Hepatitis C virus (HCV) is one of the major causes of chronic liver disease and leads to cirrhosis and hepatocarcinoma. Despite extensive research, there is still no vaccine against HCV. In order to induce an immune response in DBA/2J mice against HCV, we obtained modified mouse mesenchymal stem cells (mMSCs) simultaneously expressing five nonstructural HCV proteins (NS3-NS5B). The innate immune response to mMSCs was higher than to DNA immunization, with plasmid encoding the same proteins, and to naïve unmodified MSCs. mMSCs triggered strong phagocytic activity, enhanced lymphocyte proliferation, and production of type I and II interferons. The adaptive immune response to mMSCs was also more pronounced than in the case of DNA immunization, as exemplified by a fourfold stronger stimulation of lymphocyte proliferation in response to HCV, a 2.6-fold higher rate of biosynthesis, and a 30-fold higher rate of secretion of IFN-γ, as well as by a 40-fold stronger production of IgG2a antibodies to viral proteins. The immunostimulatory effect of mMSCs was associated with pronounced IL-6 secretion and reduction in the population of myeloid derived suppressor cells (MDSCs). Thus, this is the first example that suggests the feasibility of using mMSCs for the development of an effective anti-HCV vaccine.

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“…The superior stimulatory effect of MSC-derived exosomes on tumor angiogenesis was also addressed by different authors [110]. For example, Zhu et al demonstrated the vasculogenic role of MSC exosomes after addition to human gastric carcinoma (SGC-7901) and colon cancer (SW480) cell lines [105,111]. They found that the normal status of signaling effectors such as phosphorylated ERK1/ ERK2, Bcl-2, and VEGF proteins; alpha-smooth muscle actin (α-SMA); CXCR-4; and mouse double minute 2 homolog (MDM2) mRNA was modulated in the favor of angiogenesis in a mouse cancer model.…”

Section: Exosomal Pro-and Anti-angiogenic Factorsmentioning

confidence: 96%

The Angiogenic Paracrine Potential of Mesenchymal Stem Cells

Rezaie¹,

Heidarzadeh²,

Hassanpour³

et al. 2020

Update on Mesenchymal and Induced Pluripotent Stem Cells

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Tissue engineering and regenerative medicine are branches of biomedical sciences that facilitate the use of cells and biocompatible scaffolds in favor of tissue restoration. In this regard, restoration and maintenance of angiogenesis and blood supplementation could be an effective strategy for injured tissue removal, accelerating healing rate, and successful transplantation of cells and scaffolds into target sites. It has been elucidated that mesenchymal stem cells have the potency to promote angiogenesis via paracrine activity and trans-differentiation into the endothelial lineage. In this chapter, we highlighted the paracrine property of mesenchymal stem cells to modulate angiogenesis in the target tissues.

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Mesenchymal Stem Cells-Derived Exosomes for Wound Regeneration

Cited by 87 publications

References 97 publications

Paracrine Mechanisms of Mesenchymal Stromal Cells in Angiogenesis

Paracrine Mechanisms of Mesenchymal Stromal Cells in Angiogenesis

Genetically Modified Mouse Mesenchymal Stem Cells Expressing Non-Structural Proteins of Hepatitis C Virus Induce Effective Immune Response

The Angiogenic Paracrine Potential of Mesenchymal Stem Cells

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