Mesenchymal Stem Cells Derived from Human Bone Marrow

Gardner, Oliver F. W.; Alini, Mauro; Stoddart, Martin J.

doi:10.1007/978-1-4939-2938-2_3

Cited by 59 publications

(50 citation statements)

References 10 publications

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“…In addition, SECs injury gradually increased and vWF expression decreased. BMMSCs showed a potent ability to differentiate into endothelial cells, and promote angiogenesis, tissue repair and secretion of VEGF[10-15]; therefore, injection of BMMSCs after RLT could reduce apoptosis of hepatocytes and SECs, and promote the proliferation of hepatocytes and SECs, mainly through the effects of VEGF secreted by BMMSCs[13]. VEGF regulates the recruitment of hepatic sinusoidal endothelial progenitor cells and promotes the proliferation of SECs, which play an important role in the postoperative sinusoidal regeneration[63,64].…”

Section: Discussionmentioning

confidence: 99%

“…BMMSCs promote angiogenesis, tissue repair, and paracrine signaling[10-15], which can relieve ischemic reperfusion injury (IRI) of the liver, reduce hepatocyte injury and accelerate liver regeneration, and are involved in anti-inflammation and immunoregulation[16-21]. BMMSCs have been investigated in the field of liver, kidney, small intestine, and islet transplantation[22-27]; however, the proportion of BMMSCs surviving in the recipient’s body for more than a week is less than 1%, which has affected its use in experimental studies[22,24,28].…”

Section: Introductionmentioning

confidence: 99%

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Effects of heme oxygenase-1-modified bone marrow mesenchymal stem cells on microcirculation and energy metabolism following liver transplantation

Liu

Shen

Wang

et al. 2017

WJG

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AIMTo investigate the effects of heme oxygenase-1 (HO-1)-modified bone marrow mesenchymal stem cells (BMMSCs) on the microcirculation and energy metabolism of hepatic sinusoids following reduced-size liver transplantation (RLT) in a rat model.METHODSBMMSCs were isolated and cultured in vitro using an adherent method, and then transduced with HO-1-bearing recombinant adenovirus to construct HO-1/BMMSCs. A rat acute rejection model following 50% RLT was established using a two-cuff technique. Recipients were divided into three groups based on the treatment received: normal saline (NS), BMMSCs and HO-1/BMMSCs. Liver function was examined at six time points. The levels of endothelin-1 (ET-1), endothelial nitric-oxide synthase (eNOS), inducible nitric-oxide synthase (iNOS), nitric oxide (NO), and hyaluronic acid (HA) were detected using an enzyme-linked immunosorbent assay. The portal vein pressure (PVP) was detected by Power Lab ML880. The expressions of ET-1, iNOS, eNOS, and von Willebrand factor (vWF) protein in the transplanted liver were detected using immunohistochemistry and Western blotting. ATPase in the transplanted liver was detected by chemical colorimetry, and the ultrastructural changes were observed under a transmission electron microscope.RESULTSHO-1/BMMSCs could alleviate the pathological changes and rejection activity index of the transplanted liver, and improve the liver function of rats following 50% RLT, with statistically significant differences compared with those of the NS group and BMMSCs group (P < 0.05). In term of the microcirculation of hepatic sinusoids: The PVP on POD7 decreased significantly in the HO-1/BMMSCs and BMMSCs groups compared with that of the NS group (P < 0.01); HO-1/BMMSCs could inhibit the expressions of ET-1 and iNOS, increase the expressions of eNOS and inhibit amounts of NO production, and maintain the equilibrium of ET-1/NO (P < 0.05); and HO-1/BMMSCs increased the expression of vWF in hepatic sinusoidal endothelial cells (SECs), and promoted the degradation of HA, compared with those of the NS group and BMMSCs group (P < 0.05). In term of the energy metabolism of the transplanted liver, HO-1/BMMSCs repaired the damaged mitochondria, and improved the activity of mitochondrial aspartate aminotransferase (ASTm) and ATPase, compared with the other two groups (P <0.05).CONCLUSIONHO-1/BMMSCs can improve the microcirculation of hepatic sinusoids significantly, and recover the energy metabolism of damaged hepatocytes in rats following RLT, thus protecting the transplanted liver.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of heme oxygenase-1-modified bone marrow mesenchymal stem cells on microcirculation and energy metabolism following liver transplantation

Liu

Shen

Wang

et al. 2017

WJG

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“…Human BM-MSCs were isolated from bone marrow samples (3 donors aged 15, 18, and 37 years) after ethical approval from the cantonal ethical commission of Bern (KEK: Req-2016-00141) using previously described protocols [62]. Bone marrow samples were aspirated of vertebral bodies from each donor, after isolation, at 70-80% con uence, hBM-MSCs were harvested by trypsinization and subcultured at a density of 3 × 10 3 cells/cm 2 in Minimum Essential Medium supplemented with 10% Sera Plus bovine serum, 100-U/mL penicillin, and 100-mg/mL streptomycin, and 5ng/ml FGF and grown until passage 3.…”

Section: Embedded Hydrogels With Mesenchymal Stem Cellsmentioning

confidence: 99%

Inhibition of Hypertrophy and Improving Chondrocyte Differentiation by MMP-13 Inhibitor Small Molecule-Encapsulated in Alginate-Chondroitin Sulfate-Platelet Lysate Hydrogel

Jahangir

Eglin²,

Pötter

et al. 2020

Preprint

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Background Mesenchymal stem cells are a promising cell source for chondrogenic differentiation and have been widely used in several preclinical and clinical studies. However, they are prone to an unwanted differentiation process towards hypertrophy that limits their therapeutic efficacy. Matrix Metallopeptidase 13 (MMP-13) is a well-known factor regulated during this undesirable event. MMP-13 is a collagen degrading enzyme, which is also highly expressed in the hypertrophic zone of the growth plate and in OA cartilage. Accordingly, we investigated the effect of MMP-13 inhibition on MSC hypertrophy.Methods In this study, 5-bromoindole-2-carboxylic acid (BICA) was used as an inhibitory agent for MMP-13 expression. After identifying its optimal concentration, BICA was mixed into a hydrogel and the release rate was studied. To prepare the ideal hydrogel, chondroitin sulfate (CS) and platelet lysate (PL) were mixed with sodium alginate (Alg) at concentrations selected based on synergistic mechanical and rheometric properties. Then, four hydrogels were prepared by combining alginate (1.5%w/v), and/or CS (1%w/v) and/or PL (20%v/v). The chondrogenic potential and progression to hypertrophy of human bone marrow-derived mesenchymal stem cell (hBM-MSC) loaded hydrogels were investigated under free swelling and mechanical loading conditions, in the presence and absence of BICA.Results Viability of hBM-MSCs seeded in the four hydrogels was similar. qRT-PCR revealed that BICA could successfully inhibit MMP-13 expression, which led to an inhibition of Coll X and induction of Coll-II, in both free swelling and loading conditions. The GAG deposition was higher in the group combining BICA and mechanical stimulation.Conclusions It is concluded that BICA inhibition of MMP-13 reduces MSC hypertrophy during chondrogenesis.

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“…Cell culture 2.7.1 Cell isolation hMSCs were isolated from bone marrow aspirate with full ethical approval (Ethics Committee of University of Freiburg Medical Centre (EK-Freiburg: 135/14) as described elsewhere [24]. hMSCs were sub-cultured (3000 cells/cm 2 ) with α -MEM (Gibco) supplemented with 10% v/v Sera Plus bovine serum (PAN Biotech), 100 U/ml Penicillin, 100 ug/ml Streptomycin (Gibco) and 5 ng/ml basic Fibroblast Growth Factor (FGFb, Fitzgerald Industries International) in a humidified atmosphere of 5% CO 2 with media change every second day.…”

Section: 7mentioning

confidence: 99%

Tissue mimetic hyaluronan bioink containing collagen fibers with controlled orientation modulating cell morphology and alignment

Schwab

Hélary

Richards

et al. 2020

Preprint

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Biofabrication is providing scientists and clinicians the ability to produce engineered tissues with desired shapes, chemical and biological gradients. Typical resolutions achieved with extrusion-based bioprinting are at the macroscopic level. However, for capturing the fibrillar nature of the extracellular matrix (ECM), it is necessary to arrange ECM components at smaller scales, down to the sub-micron and the molecular level. In this study, we introduce a (bio)ink containing hyaluronan (HA) as tyramine derivative (THA) and collagen (Col). Similarly to other connective tissues, in this (bio)ink Col is present in fibrillar form and HA as viscoelastic space filler. THA was enzymatically crosslinked under mild conditions allowing simultaneous Col fibrillogenesis, thus achieving a homogeneous distribution of Col fibrils within the viscoelastic HA-based matrix. THA-Col composite displayed synergistic properties in terms of storage modulus and shear-thinning, translating into good printability. Shear-induced alignment of the Col fibrils along the printing direction was achieved and quantified via immunofluorescence and second harmonic generation. Cell-free and cell-laden constructs were printed and characterized, analyzing the influence of the controlled microscopic anisotropy on cell behavior and chondrogenic differentiation. THA-Col showed cell instructive properties modulating hMSC adhesion, morphology and sprouting from spheroids stimulated by the presence and the orientation of Col fibers. Actin filament staining showed that hMSCs embedded into aligned constructs displayed increased cytoskeleton alignment along the fibril direction. Based on gene expression of cartilage/bone markers and matrix production, hMSCs embedded into the bioink displayed chondrogenic differentiation comparable or superior to standard pellet culture by means of proteoglycan production (Safranin O staining and proteoglycan quantification) as well as increase in cartilage related genes. The possibility of printing matrix components with control over microscopic alignment brings biofabrication one step closer to capturing the complexity of native tissues.

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Mesenchymal Stem Cells Derived from Human Bone Marrow

Cited by 59 publications

References 10 publications

Effects of heme oxygenase-1-modified bone marrow mesenchymal stem cells on microcirculation and energy metabolism following liver transplantation

Effects of heme oxygenase-1-modified bone marrow mesenchymal stem cells on microcirculation and energy metabolism following liver transplantation

Inhibition of Hypertrophy and Improving Chondrocyte Differentiation by MMP-13 Inhibitor Small Molecule-Encapsulated in Alginate-Chondroitin Sulfate-Platelet Lysate Hydrogel

Tissue mimetic hyaluronan bioink containing collagen fibers with controlled orientation modulating cell morphology and alignment

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