Mesenchymal Stem Cells Isolated from Adipose and Other Tissues: Basic Biological Properties and Clinical Applications

Orbay, Hakan; Tobita, Morikuni; Mizuno, Hiroshi

doi:10.1155/2012/461718

Cited by 199 publications

(162 citation statements)

References 109 publications

(161 reference statements)

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“…Initially identified from bone marrow, MSCs have been isolated from various sources, including adipose tissue, gingiva, umbilical cord, and many other adult tissues (8). In addition to their regenerative potential, they exert profound immunomodulation by affecting proliferation, differentiation, and maturation of various immune cell types (9).…”

mentioning

confidence: 99%

Adipose-Derived Mesenchymal Stem Cells Prevent Systemic Bone Loss in Collagen-Induced Arthritis

Garimella

Kour

Piprode

et al. 2015

The Journal of Immunology

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Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammatory synovitis leading to joint destruction and systemic bone loss. The inflammation-induced bone loss is mediated by increased osteoclast formation and function. Current antirheumatic therapies primarily target suppression of inflammatory cascade with limited or no success in controlling progression of bone destruction. Mesenchymal stem cells (MSCs) by virtue of their tissue repair and immunomodulatory properties have shown promising results in various autoimmune and degenerative diseases. However, the role of MSCs in prevention of bone destruction in RA is not yet understood. In this study, we investigated the effect of adipose-derived MSCs (ASCs) on in vitro formation of bone-resorbing osteoclasts and pathological bone loss in the mouse collagen-induced arthritis (CIA) model of RA. We observed that ASCs significantly inhibited receptor activator of NF-κB ligand (RANKL)–induced osteoclastogenesis in both a contact-dependent and -independent manner. Additionally, ASCs inhibited RANKL-induced osteoclastogenesis in the presence of proinflammatory cytokines such as TNF-α, IL-17, and IL-1β. Furthermore, treatment with ASCs at the onset of CIA significantly reduced clinical symptoms and joint pathology. Interestingly, ASCs protected periarticular and systemic bone loss in CIA mice by maintaining trabecular bone structure. We further observed that treatment with ASCs reduced osteoclast precursors in bone marrow, resulting in decreased osteoclastogenesis. Moreover, ASCs suppressed autoimmune T cell responses and increased the percentages of peripheral regulatory T and B cells. Thus, we provide strong evidence that ASCs ameliorate inflammation-induced systemic bone loss in CIA mice by reducing osteoclast precursors and promoting immune tolerance.

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mentioning

confidence: 99%

Adipose-Derived Mesenchymal Stem Cells Prevent Systemic Bone Loss in Collagen-Induced Arthritis

Garimella

Kour

Piprode

et al. 2015

The Journal of Immunology

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“…However, these findings suggest that increases in the thermogenic protein UCP1, CITED1, and CD137 during the ADMSC differentiation process may result from a reduction in pRb activity. These changes indicate that EID1 generates the beige phenotype during the process of differentiation of the multipotent cells used in this work (Zeve et al 2009, Orbay et al 2012. ADMSCs may express UCP1 after several days of treatment with rosiglitazone (Elabd et al 2009).…”

Section: Discussionmentioning

confidence: 91%

Regulation of human subcutaneous adipocyte differentiation by EID1

Vargas

Shimokawa

Kaneko

et al. 2015

Journal of Molecular Endocrinology

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Increasing thermogenesis in white adipose tissues can be used to treat individuals at high risk for obesity and cardiovascular disease. The objective of this study was to determine the function of EP300-interacting inhibitor of differentiation (EID1), an inhibitor of muscle differentiation, in the induction of beige adipocytes from adipose mesenchymal stem cells (ADMSCs). Subcutaneous adipose tissue was obtained from healthy women undergoing abdominoplasty. ADMSCs were isolated in vitro, grown, and transfected with EID1 or EID1 siRNA, and differentiation was induced after 48 h by administering rosiglitazone. The effects of EID1 expression under the control of the aP2 promoter (aP2-EID1) were also evaluated in mature adipocytes that were differentiated from ADMSCs. Transfection of EID1 into ADMSCs reduced triglyceride accumulation while increasing levels of thermogenic proteins, such as PGC1a, TFAM, and mitochondrial uncoupling protein 1 (UCP1), all of which are markers of energy expenditure and mitochondrial activity. Furthermore, increased expression of the beige phenotype markers CITED1 and CD137 was observed. Transfection of aP2-EID1 transfection induced the conversion of mature white adipocytes to beige adipocytes, as evidenced by increased expression of PGC1a, UCP1, TFAM, and CITED1. These results indicate that EID1 can modulate ADMSCs, inducing a brown/beige lineage. EID1 may also activate beiging in white adipocytes obtained from subcutaneous human adipose tissue.

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“…The epidermis, hair, and the gastrointestinal tract all are proper models of tissues with niches containing stem cells that can supply these cells throughout typical cellular turnover (21).…”

Section: Detecting Mesanchymal Stem Cellsmentioning

confidence: 99%

“…MSCs have been isolated from a variety of tissues including adipose tissue, tendon, periodontal ligament, synovial membranes, trabecular bone, bone marrow, embryonic tissues, the nervous system, skin, periosteum, muscle, first-trimester blood and fetal pancreas (16,21,23).…”

Section: Detecting Mesanchymal Stem Cellsmentioning

confidence: 99%

“…These surface antigen expression allows for rapid detection of the MSCs, according to the ISCT protocol, to identify the MSCs, the cells should be CD105+, which is known as the endoglin, recognized by the MAb SH2, the other CD antigens that are commonly expressed by these cells are the CD73 and CD90 which are known as the ecto 5' nucleotidase and Thy-1, respectively. Moreover the lack of hematopoietic antigen expression, including CD45, CD19, CD14, is an additional criterion of MSCs detection (21,24,25). …”

Section: Mesanchymal Stem Cell Bio Markersmentioning

confidence: 99%

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Literature Review of Adipose-derived Mesanchymal Cells from History to Approaches

Amirkhani

Zare

Shoaei-Hassani

et al. 2016

Iran Red Crescent Med J

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Context: After about 40 years from the discovery of bone marrow stem cells, the adipose stem cells (ASCs) were identify and the applications of these cells in different fields including the medical and biomedical fields have always attracted the attention of the scientists. The great quantity of stem cells obtained from adipose tissue allows the scientists to rapidly apply these primary cells without culture expansion. Evidence Acquisition: Human has different types of fat tissues and each has its own applications, which means that the ASCs are harvested from different niches and each could be applied for different purposes. There are also specific biomarkers on the surface of the stem cells which is specific for every kind of these cells and the ASCs have also their own surface markers which help to easily detect. Results: Already the ASCs are clinically applied in many other purposes but the first clinical application of ASCs was reported in 2004 for the treatment of traumatic calvarial deficiency in a 7-year-old girl. However in the last decade a huge number of studies has been done on ASCs and the ASCs are used for different reasons and in different medical fields including the treatment of cardiovascular and immune diseases, for healing the wound scars and also is applied for skin rejuvenate, radiation injury, scar remodeling, and skin engineering. Conclusions: This review was to focus on the roles of ASCs in different applications and also provide the researcher general information about the history and current applications of ASCs. We found that the ASCs have a potential role to be used for the treatment of different sorts of disorders, with the less side effects and is also a new approach toward new remedies.

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Mesenchymal Stem Cells Isolated from Adipose and Other Tissues: Basic Biological Properties and Clinical Applications

Cited by 199 publications

References 109 publications

Adipose-Derived Mesenchymal Stem Cells Prevent Systemic Bone Loss in Collagen-Induced Arthritis

Adipose-Derived Mesenchymal Stem Cells Prevent Systemic Bone Loss in Collagen-Induced Arthritis

Regulation of human subcutaneous adipocyte differentiation by EID1

Literature Review of Adipose-derived Mesanchymal Cells from History to Approaches

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