Mesenchymal stem cells transplantation could be beneficial for treatment of experimental ischemic stroke in rats

Pavlichenko, N. N.; Sokolova, I. B.; Vijde, Svetlana; Shvedova, E. V.; Alexandrov, Georgy; Krouglyakov, Peter; Fedotova, O. R.; Gilerovich, E. G.; Polyntsev, D. G.; Otellin, V. A.

doi:10.1016/j.brainres.2008.06.123

Cited by 106 publications

(66 citation statements)

References 42 publications

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“…Consistent with previous studies, MCAO resulted in an increased escape latency and path length relative to sham and unoperated animals in a watermaze spatial reference-memory task (Markgraf et al, 1992;Pavlichenko et al, 2008;Sadamoto et al, 1998;Shinoda et al, 1996;Smith et al, 1997;Wang et al, 2010;Xu et al, 2009;Yonemori et al, 1996;Yun et al, 2007;Zhang et al, 2010). However, from close scrutiny of the behavior of MCAO animals in the watermaze, the present study indicates that maintained focal ischemia within the MCA territory has little effect on the acquisition and retention of spatial reference memory.…”

Section: Discussionsupporting

confidence: 91%

“…A number of watermaze studies have been performed to determine the effect of MCAO on learning and memory deficits using either the Tamura model of permanent MCAO by electrocoagulation (e.g., Markgraf et al, 1992;Pavlichenko et al, 2008;Sadamoto et al, 1998;Shinoda et al, 1996;Smith et al, 1997;Yonemori et al, 1996) or the intraluminal thread model (e.g., Hayase et al, 2009;Kawanishi et al, 2010;Noh et al, 2011;Xu et al, 2009;Yun et al, 2007). It is important to note that with intraluminal thread models, particularly in mice, filament insertion can lead to loss of blood supply to the hippocampus (McColl et al, 2004), therefore although intraluminal thread is used as a model of MCAO, it is in fact capable of producing more widespread ischemia affecting other major blood vessel territories.…”

Section: Discussionmentioning

confidence: 99%

“…Group 1 underwent a proximal permanent left MCAO (n = 10). A standard model of MCAO (the electrocoagulation Tamura model; Tamura et al, 1981) used in previous watermaze studies (Markgraf et al, 1992;Pavlichenko et al, 2008;Sadamoto et al, 1998;Shinoda et al, 1996;Smith et al, 1997;Yonemori et al, 1996) was adopted where ischemia encompasses frontal, parietal, and perirhinal cortices and striatum, as described previously (Bingham et al, 2005). Anesthesia was induced with 5% halothane and the animals were then mechanically ventilated and anesthesia maintained with 1% to 2% halothane in 100% oxygen.…”

Section: Animalsmentioning

confidence: 99%

“…Understanding these functional deficits is essential for development of future therapies. Many experimental studies have used watermaze tasks to better understand spatial learning and memory deficits after MCA occlusion (MCAO) and to assess potential future therapies (Hayase et al, 2009;Kawanishi et al, 2010;Markgraf et al, 1992;Noh et al, 2011;Pavlichenko et al, 2008;Sadamoto et al, 1998;Shinoda et al, 1996;Smith et al, 1997;Wang et al, 2010;Xu et al, 2009;Yonemori et al, 1996;Yun et al, 2007;Zhang et al, 2010). However, in rodent experimental stroke models, investigation of deficits in spatial memory using the Morris watermaze may be confounded by coexisting sensory and motor impairments.…”

Section: Introductionmentioning

confidence: 99%

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Watermaze Performance after Middle Cerebral Artery Occlusion in the Rat: The Role of Sensorimotor versus Memory Impairments

Bingham

Martin

Macrae

et al. 2012

J Cereb Blood Flow Metab

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In rodent stroke models, investigation of deficits in spatial memory using the Morris watermaze may be confounded by coexisting sensory or motor impairments. To target memory specifically, we devised a watermaze protocol to minimize the impact of sensory and motor impairments in female Lister-hooded rats exposed to proximal electrocoagulation of the middle cerebral artery (MCAO). Rats were trained in a reference-memory task comprising 4 trials/day; trial 1 being a probe trial (platform absent for the first 60 seconds). Training ended once animals reached a strict criterion based on the probe-trial performance. Memory retention was tested 1, 7, and 28 days later. The MCAO did not affect the number of days to reach criterion during acquisition or the time spent in target quadrant during retention testing, compared with sham or unoperated rats. However, MCAO rats showed slightly poorer accuracy in crossing the platform location and increased thigmotactic swimming compared with controls. Our results show that spatial memory deficits are minimal in this rodent stroke model, and suggest that previously published watermaze impairments are attributable to sensory and motor deficits but not memory deficits. We recommend using probe trials and training to a predetermined performance criterion in future studies assessing watermaze memory deficits in rodent stroke models.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Animalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Watermaze Performance after Middle Cerebral Artery Occlusion in the Rat: The Role of Sensorimotor versus Memory Impairments

Bingham

Martin

Macrae

et al. 2012

J Cereb Blood Flow Metab

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show abstract

“…When the cells grew until confluent, they were lifted using 0.25% trypsin and 0.02% EDTA in phosphate-buffered saline (PBS). The cells were cultured at a density of 5-10×10 5 cells/cm 2 and used in all the experiments.…”

Section: Isolation and Expansion Of Mscsmentioning

confidence: 99%

Cerebrospinal Fluid following Cerebral Ischemia Accelerates the Proliferation of Bone Marrow Stromal Cells in vitro

et al. 2010

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The role of astrocytes in mediating exogenous cell‐based restorative therapy for stroke

Liu

Xin

et al. 2013

Glia

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Astrocytes have not been a major therapeutic target for the treatment of stroke, with most research emphasis on the neuron. Given the essential role that astrocytes play in maintaining physiological function of the central nervous system and the very rapid and sensitive reaction astrocytes have in response to cerebral injury or ischemic insult, we propose to replace the neurocentric view for treatment with a more nuanced astrocytic centered approach. In addition, after decades of effort in attempting to develop neuroprotective therapies, which target reduction of the ischemic lesion, there are no effective clinical treatments for stroke, aside from thrombolysis with tissue plasminogen activator, which is used in a small minority of patients. A more promising therapeutic approach, which may affect nearly all stroke patients, may be in promoting endogenous restorative mechanisms, which enhance neurological recovery. A focus of efforts in stimulating recovery post stroke is the use of exogenously administered cells. The present review focuses on the role of the astrocyte in mediating the brain network, brain plasticity, and neurological recovery post stroke. As a model to describe the interaction of a restorative cell-based therapy with astrocytes, which drives recovery from stroke, we specifically highlight the subacute treatment of stroke with multipotent mesenchymal stromal cell therapy.

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Mesenchymal stem cells transplantation could be beneficial for treatment of experimental ischemic stroke in rats

Cited by 106 publications

References 42 publications

Watermaze Performance after Middle Cerebral Artery Occlusion in the Rat: The Role of Sensorimotor versus Memory Impairments

Watermaze Performance after Middle Cerebral Artery Occlusion in the Rat: The Role of Sensorimotor versus Memory Impairments

Cerebrospinal Fluid following Cerebral Ischemia Accelerates the Proliferation of Bone Marrow Stromal Cells in vitro

The role of astrocytes in mediating exogenous cell‐based restorative therapy for stroke

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