Mesoporous bioactive glass surface modified poly(lactic-co-glycolic acid) electrospun fibrous scaffold for bone regeneration

Abstract

A mesoporous bioactive glass (MBG) surface modified with poly(lactic-co-glycolic acid) (PLGA) electrospun fibrous scaffold for bone regeneration was prepared by dip-coating a PLGA electrospun fibrous scaffold into MBG precursor solution. Different surface structures and properties were acquired by different coating times. Surface morphology, chemical composition, microstructure, pore size distribution, and hydrophilicity of the PLGA-MBG scaffold were characterized. Results of scanning electron microscopy indicated that MBG surface coating made the scaffold rougher with the increase of MBG content. Scaffolds after MBG modification possessed mesoporous architecture on the surface. The measurements of the water contact angles suggested that the incorporation of MBG into the PLGA scaffold improved the surface hydrophilicity. An energy dispersive spectrometer evidenced that calcium-deficient carbonated hydroxyapatite formed on the PLGA-MBG scaffolds after a 7-day immersion in simulated body fluid. In vitro studies showed that the incorporation of MBG favored cell proliferation and osteogenic differentiation of human mesenchymal stem cells on the PLGA scaffolds. Moreover, the MBG surface-modified PLGA (PLGA-MBG) scaffolds were shown to be capable of providing the improved adsorption/release behaviors of bone morphogenetic protein-2 (BMP-2). It is very significant that PLGA-MBG scaffolds could be effective for BMP-2 delivery and bone regeneration.

Mesoporous bioactive glass combined with graphene oxide scaffolds for bone repair

“…The regeneration process of bone defects primarily relies on a physical bridge between defect ends and the chemical guidance of bioactive molecules and proteins. Bioactive ceramics have been widely accepted and used as a successful biomaterial in studies of bone repair and drug carriers 1-3, 6, 30, 31, 36-39. Particularly in the bone tissue engineering field, various bioactive factors like DMOG, VEGF and BMP-2 encapsulated in mesoporous bioactive glasses have already been tested to enhance the function of osteogenesis and angiogenesis 3, 6-8.…”

“…New bone formation and vascularization are often limited to the periphery of the scaffolds due to the damaged blood vessels in the center of the defect 4, 5. Methods that aim to address this issue have been explored, including the use of an expensive recombinant pro-angiogenic vascular endothelial growth factor (VEGF) and bone morphogenetic protein (BMP) in combination with tissue engineering scaffolds 6-8. These have strong properties of angiogenesis and osteogenesis, respectively.…”

Effect of bioactive glass nanoparticles on biological properties of PLGA/collagen scaffold

“…Moreover, it has been observed that this effect (i.e., stimulation of angiogenic growth factors) is dose dependent, and high concentration of BG has a negative effect on growth factor secretion. Besides biological properties, surface coating of electrospun PLGA fibers with mesoporous bioactive glass improved the capability of scaffold for BMP-2 delivery (Li et al 2015 ). Addition of BG to collagen has been studied in some research.…”