Mesoporous carbon-enriched palladium nanostructures with redox activity for enzyme-free electrochemical immunoassay of brevetoxin B

Lin, Youxiu; Zhou, Qian; Lin, Yuping; Lu, Minghua; Tang, Dianping

doi:10.1016/j.aca.2015.06.010

Cited by 38 publications

(10 citation statements)

References 38 publications

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“…18,19 In contrast with nanolabels, unfavorably, enzymebased labeling strategies are susceptible to interference and assay conditions during the signal-generation stage, e.g., pH, temperature and the instability caused by structural unfolding. 20 Inspiringly, the emergence of nanocatalysts opens a new horizon for the development of advanced nanolabels for signal amplification. 21,22 One main merit of using nanomaterials is that one can control and tailor their properties in a very predictable manner to meet the needs of specific application.…”

Section: Introductionmentioning

confidence: 99%

Sensitive electrochemical immunoassay with signal enhancement based on nanogold-encapsulated poly(amidoamine) dendrimer-stimulated hydrogen evolution reaction

Sun

2015

Analyst

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A new electrochemical immunosensor with signal enhancement was designed for sensitive detection of disease-related protein (human carbohydrate antigen 19-9, CA 19-9 used in this case). The assay was carried out on a capture antibody-modified screen-printed carbon electrode with a sandwich-type mode by using detection antibody-functionalized nanogold-encapsulated poly(amidoamine) dendrimer (AuNP-PAAD). The AuNP-PAAD was first synthesized through the in situ reduction method and functionalized with the polyclonal rabbit anti-human CA 19-9 antibody. Upon target CA 19-9 introduction, a sandwiched immunocomplex could be formed between the capture antibody and detection antibody. Accompanying the AuNP-PAAD, the electrocatalytic activity of the carried gold nanoparticles toward the hydrogen evolution reaction (HER) allowed the rapid quantification of the target analyte on the electrode. The amplified electrochemical signal mainly derived from AuNP-catalyzed HER in an acidic medium. Under optimal conditions, the immuno-HER assay displayed a wide dynamic concentration range from 0.01 to 300 U mL(-1) toward target CA 19-9 with a detection limit (LOD) of 6.3 mU mL(-1). The reproducibility, precision, specificity and stability of our strategy were acceptable. Additionally, the system was further validated by assaying 13 human serum specimens, giving well matched results obtained from the commercialized enzyme-linked immunosorbent assay (ELISA) method.

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Section: Introductionmentioning

confidence: 99%

Sensitive electrochemical immunoassay with signal enhancement based on nanogold-encapsulated poly(amidoamine) dendrimer-stimulated hydrogen evolution reaction

Sun

2015

Analyst

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“…5B). Obviously, the LOD of our strategy was even lower than those of cardiomyocyte-based potential biosensor (1.5 ng mL -1 ) (Wang et al, 2015), enzyme-free electrochemical immunoassay (5 pg mL -1 ) (Lin et al, 2015b), aptamer-based competitive electrochemical biosensor (106 pg mL -1 ) (Eissa et al, 2015), Ag(I)-TMB-based colorimetric immunoassay (0.1 ng kg -1 ) (Lai et al, 2015), hollow gold nanosphere-based immunodipstick (0.1 ng mL -1 ) (Zhang et al, 2014), homogenous electrochemical immunoassay (6 pg mL -1 ) , displacement-type quartz crystal microbalance immunoassay (1.0 pg mL -1 ) (Tang et al, 2013), capillary electrophoresis-based immunoassay (0.1 ng mL -1 ) , glucose meter-based assay (0.1 ng mL -1 ) (Gao et al, 2014b) and commercialized BTB ELISA kit from Abraxis LLC Inc. (50 ng kg -1 ). Due to the legal limit of brevetoxin (≤ 80 μg of toxin per 100 g of shellfish tissue) (Hunt et al, 1979;Trainer et al, 1991), the Fenton reaction-based colorimetric immunoassay could completely meet the need of BTB monitoring in seafood.…”

Section: Analytical Performance Of Fenton Reaction-based Colorimetricmentioning

confidence: 84%

Fenton reaction-based colorimetric immunoassay for sensitive detection of brevetoxin B

Lai

Wei

Zhuang

et al. 2016

Biosensors and Bioelectronics

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We designed a new colorimetric immunoassay for sensitive monitoring of brevetoxin B (BTB) using enzyme-controlled Fenton reaction with a high-resolution 3,3',5,5'-tetramethylbenzidine (TMB)-based visual colored system. Upon addition of hydrogen peroxide (H2O2), the equivalent iron(II) could be first converted into iron(III) and free hydroxyl radical (•OH) via the classical Fenton reaction. Then the as-produced iron(III) and •OH could cause a perceptible change from colorless to blue with the increasing H2O2 concentration in the presence of TMB. Based on Fenton reaction-triggered visual colored system, a novel competitive-type colorimetric enzyme immunoassay was developed for the quantitative screening of target BTB on the bovine serum albumin-BTB-modified magnetic bead using glucose oxidase/anti-BTB antibody-labeled gold nanoparticle as the signal-transduction tag. Upon target BTB introduction, the analyte competed with the conjugated BTB on the magnetic bead for anti-BTB antibody on gold nanoparticle. The carried glucose oxidase with the gold nanoparticle could implement the oxidation of glucose to produce H2O2, and the generated H2O2 promoted the above-mentioned Fenton reaction for color development. Under the optimal conditions, the absorbance decreased with the increasing target BTB in the range from 0.1 to 150 ng kg(-1) with a low detection limit (LOD) of 0.076 ng kg(-1). The LOD was 500-fold lower than that of commercialized Abraxis BTB ELISA kit. Non-specific adsorption was not observed. The precision, reproducibility and specificity were acceptable. Finally, the method accuracy was also validated for monitoring spiked seafood samples, giving results well matched with the referenced brevetoxin ELISA kit.

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“…In the state of the art, most highly sensitive PEC sensing strategies need unstable and batch variable biological reagents and/or complex photoactive nanocomposites as either signal reporting tags or electrode-modified materials. Their unstable and hard to control nature might increase the uncertainties in assay results, complicate experimental operation, and increase the costs. − The development of a PEC immunoassay method with simple operation, low cost, but effective signal transduction is highly demanded.…”

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confidence: 99%

Polydopamine with Tailorable Photoelectrochemical Activities for the Highly Sensitive Immunoassay of Tumor Markers

et al. 2021

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A simple and highly sensitive photoelectrochemical (PEC) immunoassay sensor was fabricated by using the two forms of polydopamine (PDA), the thin film and nanosphere, to serve as the photoelectrode-modified material and signal reporting label, respectively. The two forms of PDA show similar light absorption behavior but totally different PEC activities. The PDA film can extend the light absorption from the ultraviolet to near infrared light range, transfer a photoelectron to TiO2 nanoparticles and the underlying photoelectrode, and largely amplify the photocurrent response. However, the PDA nanospheres have insignificant photoelectron transport ability. When they are brought close to the PDA film and TiO2 nanocomposite-modified electrode via the sandwich immunoreaction, they function like a black hole to compete with the PDA film for light absorption, resist the access of the electron donor to regenerate the photoactive material, and capture the photoelectron generated from the PDA film. Besides, the heat generated from the PDA nanospheres also contributes to the photocurrent decrease. The PDA nanospheres with multiple quenching effects on the PDA film greatly decrease the photocurrent signal and lead to a highly sensitive PEC immunosensing strategy. Under optimal conditions, a wide linear range from 0.1 to 106 pg·mL–1 is obtained toward carcinoembryonic antigen, with a low limit of detection of 40 fg·mL–1. Besides, the PDA with excellent biosafety can be readily assembled with proteins, which thus simplifies the preparation procedures and decreases the costs. All these features indicate that the whole PDA-based PEC sensing strategy may have great application prospects for the point-of-care assay of various kinds of tumor markers.

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Mesoporous carbon-enriched palladium nanostructures with redox activity for enzyme-free electrochemical immunoassay of brevetoxin B

Cited by 38 publications

References 38 publications

Sensitive electrochemical immunoassay with signal enhancement based on nanogold-encapsulated poly(amidoamine) dendrimer-stimulated hydrogen evolution reaction

Sensitive electrochemical immunoassay with signal enhancement based on nanogold-encapsulated poly(amidoamine) dendrimer-stimulated hydrogen evolution reaction

Fenton reaction-based colorimetric immunoassay for sensitive detection of brevetoxin B

Polydopamine with Tailorable Photoelectrochemical Activities for the Highly Sensitive Immunoassay of Tumor Markers

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