Mesoporous silica-mediated controllable electrochemiluminescence quenching for immunosensor with simplicity, sensitivity and tunable detection range

Xiao, Yingying; Chen, Suhua; Zhang, Simeng; Wang, Guofang; Yi, Haomin; Xin, Gui-Zhong; Yang, Xiaoping

doi:10.1016/j.talanta.2021.122399

Cited by 12 publications

(10 citation statements)

References 42 publications

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“…The signal enhancement in Ru(bpy) 3 2+ @liposome solution after the addition of nisin is due to the disruption of the liposome structure, resulting in the leakage of Ru(bpy) 3 2+ . When Ru(bpy) 3 2+ (~1.2 nm) is released, it could be enriched by the negatively charged nanochannel array (2–3 nm in diameter) because of the electrostatic interaction between VMSF and Ru(bpy) 3 2+ ( 47 – 51 ). Thus, the released Ru(bpy) 3 2+ could get to the ITO surface, leading to significant ECL signal.…”

Section: Resultsmentioning

confidence: 99%

Novel electrochemical and electrochemiluminescence dual-modality sensing platform for sensitive determination of antimicrobial peptides based on probe encapsulated liposome and nanochannel array electrode

2022

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With the increasing application of antimicrobial peptides (AMPs) to replace antibiotics in medicine, food and agriculture, it is highly desired to develop a fast, reliable, and convenient strategy for sensitive detection of AMPs. Herein, a novel electrochemical (EC) and electrochemiluminescence (ECL) dual-modality sensing platform was developed based on probe encapsulated liposomes and nanochannel array modified electrodes, which enables sensitive determination of nisin in food samples. The bifunctional probe with both EC and ECL signals, tris(2,2-bipyridyl) dichlororuthenium (II) (Ru(bpy)32+), was chosen to be easily encapsulated in liposomes (Ru(bpy)32+@liposome). Based on the unique sterilization mechanism that AMPs can disrupt cell membranes, Ru(bpy)32+@liposome can be destroyed by nisin and release a large number of Ru(bpy)32+ probes. Vertically-ordered mesoporous silica-nanochannel film (VMSF) modified ITO electrodes (VMSF/ITO) prepared by electrochemically assisted self-assembly (EASA) method were applied as the sensing electrode. Due to the efficient enrichment of Ru(bpy)32+ by the negatively charged nanochannel arrays, VMSF/ITO enables detection of the EC/ECL signals of the released Ru(bpy)32+ probes with ultrahigh sensitivity. In consequence, sensitive dual-modality detection of nisin was achieved by the combination of Ru(bpy)32+@liposome and VMSF/ITO. The developed sensing system can realize sensitive determination of nisin in ECL mode in the concentration range of 10 ng/ml to 50 μg/ml with a limit of detection (LOD) of 9.3 ng/ml, or in EC mode from 800 ng/ml to 100 μg/ml with a LOD of 70 ng/ml. Combined with the excellent anti-fouling and anti-interference performance of VMSF, rapid and sensitive detection of nisin in milk or egg white was also achieved by the sensor.

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Section: Resultsmentioning

confidence: 99%

Novel electrochemical and electrochemiluminescence dual-modality sensing platform for sensitive determination of antimicrobial peptides based on probe encapsulated liposome and nanochannel array electrode

2022

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“…The whole detection process is about 2 min, and 18 samples can be detected simultaneously. The ECL sensor can be applied for different phenols analysis, such as phenol, 23 and acetaminophen. 26 The relationship of different phenols and ECL intensity is below.…”

Section: Resultsmentioning

confidence: 99%

“…Due to MSNs' robust nanostructure and extraordinary properties including high surface area, and controlled pore size, they have attracted considerable attention for the on-site and real-time analysis. [19][20][21] Because of the deprotonation of silanol groups with an isoelectric point of 2 $ 3, 22,23 MSNs repel the transport of anion while favoring that of cation, and are employed to enrich the luminophores. MSNs membranes can amplify the ECL signal due to electrostatic attraction between silanol groups and Ru(bpy) 3 2+ .…”

Section: Introductionmentioning

confidence: 99%

Simple, rapid, and visual electrochemiluminescence sensor for on-site catechol analysis

Chen

Lei

et al. 2022

RSC Adv.

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“…Among the developed SSN, vertically ordered mesoporous silica nanochannel film (VMSF) exhibits high chemical and thermal stability, ordered and vertically aligned nanochannels (nanopores), uniform pore size distribution, high pore density, and ease of modification ( Nasir et al, 2018 ; Jiokeng et al, 2019 ; Ullah et al, 2021 ; Walcarius, 2021 ). The ultrasmall (usually 2–3 nm) and high-density (up to 4∼8 × 10 12 /cm 2 ) nanochannel arrays make it possible to significantly improve the sensor performance as electrode modification materials ( Mi et al, 2020 ; Li et al, 2021a ; Asadpour et al, 2021 ; Li et al, 2021b ; Xiao et al, 2021 ). On the one hand, a large number of silanol groups (Si-OH) with low pKa (∼2) endow the VMSF with a negatively charged surface, which can accelerate the transfer of positively charged molecules to the electrode surface, leading to a significant enrichment effect.…”

Section: Introductionmentioning

confidence: 99%

A Flexible Electrochemiluminescence Sensor Equipped With Vertically Ordered Mesoporous Silica Nanochannel Film for Sensitive Detection of Clindamycin

Wei

et al. 2022

Front. Chem.

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Fast, convenient, and highly sensitive detection of antibiotic is essential to avoid its overuse and the possible harm. Owing to enrichment effect and antifouling ability of ultrasmall nanochannels, the vertically ordered mesoporous silica nanochannel film (VMSF) has great potential in the development of the facile electrochemiluminescence (ECL) sensor for direct and sensitive analysis of antibiotics in complex samples. In this study, we demonstrated a flexible ECL sensor based on a cost-effective electrode covered with a VMSF for sensitive detection of clindamycin. Polyethylene terephthalate coated with indium tin oxide (PET-ITO) is applied as a flexible electrode to grow VMSF using the electrochemically assisted self-assembly (EASA) method. The negatively charged VMSF nanochannels exhibit significant enrichment toward the commonly used cationic ECL luminophores, tris(2,2-bipyridyl) dichlororuthenium (II) (Ru (bpy)32+). Using the enhanced ECL of Ru (bpy)32+ by clindamycin, the developed VMSF/PET-ITO sensor can sensitively detect clindamycin. The responses were linear in the concentration range of 10 nM–25 μM and in the concentration range of 25–70 μM. Owing to the nanoscale thickness of the VMSF and the high coupling stability with the electrode substrate, the developed flexible VMSF/PET-ITO sensor exhibits high signal stability during the continuous bending process. Considering high antifouling characteristic of the VMSF, direct analysis of clindamycin in a real biological sample, human serum, is realized.

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Mesoporous silica-mediated controllable electrochemiluminescence quenching for immunosensor with simplicity, sensitivity and tunable detection range

Cited by 12 publications

References 42 publications

Novel electrochemical and electrochemiluminescence dual-modality sensing platform for sensitive determination of antimicrobial peptides based on probe encapsulated liposome and nanochannel array electrode

Novel electrochemical and electrochemiluminescence dual-modality sensing platform for sensitive determination of antimicrobial peptides based on probe encapsulated liposome and nanochannel array electrode

Simple, rapid, and visual electrochemiluminescence sensor for on-site catechol analysis

A Flexible Electrochemiluminescence Sensor Equipped With Vertically Ordered Mesoporous Silica Nanochannel Film for Sensitive Detection of Clindamycin

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