Messen und Rechnen im pharmakologischen Laboratorium mit einem speziellen Zahlensystem (WL24-System)

Hackenberg, U.

doi:10.1007/bf00246706

Cited by 15 publications

(1 citation statement)

References 3 publications

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“…Cellular cytotoxicity of lotus petal extracts was evaluated by neutral red uptake assay [37], whereby 5 × 104 cells/well of isolated monocytes were plated in a 96-well plate (SPL Life Sciences Co., Ltd., Gyeonggi-do, Korea) and allowed to rest for 24 h at 37 • C and 5% CO 2 in humidified incubator. Meanwhile, lotus petal extracts were diluted geometrically to concentrations of 1-10,000 µg/mL [38] with complete RPMI medium (Thermo Fisher Scientific, Waltham, MA, USA).…”

Section: Evaluation Of Cellular Cytotoxicity By Neutral Red Uptake Assaymentioning

confidence: 99%

Phytochemicals and Immunomodulatory Effect of Nelumbo nucifera Flower Extracts on Human Macrophages

Sranujit

Noysang

Tippayawat

et al. 2021

Plants

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This research characterizes phytochemicals inherent in lotus flower and investigates the antioxidant and immunomodulatory activity of ethyl acetate (EA) and ethyl alcohol (ET) lotus petal extracts. In the experiment, human monocytes-derived macrophages were stimulated by lipopoly-saccharide to mimic bacteria-induced inflammation. The results showed that ferulic acid, couma-rin, and chlorogenic acid were three dominant polyphenols. The EA and ET lotus petal extracts also possessed high antioxidant capability. Furthermore, the extracts exhibited immunomodulatory properties by suppressing TNF-α secretion in inflammatory-induced human macrophages by in-hibiting NF-κB-dependent inflammatory response. In essence, the lotus petal extracts possess reme-dial attributes beneficial to individuals afflicted with declined immune functions.

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Section: Evaluation Of Cellular Cytotoxicity By Neutral Red Uptake Assaymentioning

confidence: 99%

Phytochemicals and Immunomodulatory Effect of Nelumbo nucifera Flower Extracts on Human Macrophages

Sranujit

Noysang

Tippayawat

et al. 2021

Plants

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Transformation von Zufallsvariablen

Grimm¹

1960

Biom. J.

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The Validated Embryonic Stem Cell Test with Murine Embryonic Stem Cells

Walker

McClelland-Descalzo

Nieden

2018

Methods in Molecular Biology

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Birth defects are the leading cause of infant mortality in the USA, yet the causes of most of these conditions are unknown. While a combination of genetic and environmental factors are suspected in most cases, little information exists about the health risks that prenatal exposure to many common chemicals poses for the fetus. Thus, development and refinement of procedures that can accurately predict embryotoxicity of compounds is important for curtailing the number of infants born with birth defects. The embryonic stem cell test (EST) is a procedure that utilizes comparison of cytotoxicity in embryonic and adult cells and inhibition of differentiation to predict embryotoxicity of compounds tested. Because of its use of existing cell lines, the EST dramatically reduces the need for animal test subjects in toxicity testing. In addition, because of its use of inhibition of differentiation as an endpoint, the EST is extremely versatile in the range of complications it can test for. In this chapter, procedures for use of the validated embryonic stem cell test with the traditional cardiomyocyte differentiation endpoint are explained. The protocol includes discussion of routine stem cell culture, the cardiomyocyte differentiation procedure, and methods for utilization of molecular endpoints for assessing embryotoxicity of compounds.

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Messen und Rechnen im pharmakologischen Laboratorium mit einem speziellen Zahlensystem (WL24-System)

Cited by 15 publications

References 3 publications

Phytochemicals and Immunomodulatory Effect of Nelumbo nucifera Flower Extracts on Human Macrophages

Phytochemicals and Immunomodulatory Effect of Nelumbo nucifera Flower Extracts on Human Macrophages

Transformation von Zufallsvariablen

The Validated Embryonic Stem Cell Test with Murine Embryonic Stem Cells

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